An Integrated Approach to Improving Efficiency in Photosynthetic Microbial Systems

Cyanobacteria-based biotechnology is regarded as a promising opportunity for renewable bioenergy and bioproducts. As cyanobacteria are photosynthetic microorganisms, they only require sunlight, carbon dioxide, nutrients, and water to grow, and can be cultivated using non-arable land and non-potable water. These characteristics, along with their rapid growth rates and amenability to genetic modifications, merit research of cyanobacteria for roles in mitigating greenhouse gas emissions and carbon capture and sequestration. Despite these favourable attributes, cyanobacterial bioenergy has yet to become successful at an industrial scale. This thesis explores, through use of metagenomics, metaproteomics, growth experiments, and modelling, fundamental and applied strategies to improve the productivity and feasibility of cyanobacteria in biotechnology. A photosynthetic microbial mat, sourced from highly productive haloalkaline soda lakes, was previously used as inoculum for enrichment of a mono-cyanobacterial microbial consortium. In this thesis, the microbial composition and function of the productive haloalkaline lakes of origin were analyzed using metagenomics and metaproteomics (Chapter 2). This analysis showed high diversity and functional redundancy within the mat community, and suggested approaches for niche differentiation between phototrophic species, as well as mechanisms for lateral gene transfer and biogeographic dispersal. In Chapter 3, the cyanobacterial enrichment culture was used to conduct growth experiments in conjunction with red light transmitting filters, composed of organic semiconducting materials with the potential to produce electricity. These growth experiments were used to model photosynthesis and to determine under which conditions electricity-producing light filters could be advantageous to photosynthetic growth and overall energy output. Lastly, in Chapter 4, the cyanobacterial enrichment culture underwent a prolonged dark and anoxic incubation, similar to what might be experienced in their natural lake habitat. This incubation resulted in the lysis of cyanobacterial cells and release of a highly valued pigment compound, phycocyanin. The molecular mechanism behind the lytic process was investigated using metagenomics and metaproteomics. In conclusion, this body of work examined fundamental microbiological and ecological processes in a highly productive photosynthetic mat and used biological principles to facilitate improvement of cyanobacterial biotechnology systems.

Name thesis:degree_name

Doctor of Philosophy (PhD)

Discipline thesis:degree_discipline

Geoscience

Grantor dc:publisher.institution

Science

Year dc:date.issued

2021

Author dc:creator

• Zorz, Jacqueline

Advisor dc:contributor.advisor

• Strous, Marc

Committee members dc:contributor.committeemember

• Mayer, Bernhard
• Larter, Steve
• Welch, Greg
• Gieg, Lisa
• Hallam, Steven

• renewable energy
• photosynthesis
• cyanobacteria
• algae
• biotechnology
• bioenergy

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/38906

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:1880/113467