{"id":{"repo_id":"calgary","oai_identifier":"oai:ucalgary.scholaris.ca:11023/1994"},"canonical_url":"https://search.dev.ndltd.org/etd/calgary/oai:ucalgary.scholaris.ca:11023/1994","repository":{"repo_id":"calgary","name":"University of Calgary","base_url":"https://ucalgary.scholaris.ca/server/oai/request"},"display":{"title":"Nkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells","abstract":"Vascular stabilization is the process during vascular development where the immature endothelial cell tube gains structural support through the recruitment and adhesion of perivascular mural cells; pericytes and vascular smooth muscle cells (vSMC). When vessels are not well supported by mural cells hemorrhage can occur. Nkx3.2 deficient zebrafish embryos hemorrhage, have reduced expression of a mural cell marker, and the endothelial layer makes less contact with mural cells compared with controls. I found that nkx3.2 regulates the expression of two genes with known roles in neural crest migration and specification; pdgfrα and sox10. Further, nkx3.2 functions autonomously in neural crest cells, where knockdown of nkx3.2 causes abnormal migration of neural crest cells in the head. These results show that nkx3.2 regulates genes involved in neural crest migration and in turn supports recruitment of mural cells to mediate vascular stabilization.","abstract_html":"Vascular stabilization is the process during vascular development where the immature endothelial cell tube gains structural support through the recruitment and adhesion of perivascular mural cells; pericytes and vascular smooth muscle cells (vSMC). When vessels are not well supported by mural cells hemorrhage can occur. Nkx3.2 deficient zebrafish embryos hemorrhage, have reduced expression of a mural cell marker, and the endothelial layer makes less contact with mural cells compared with controls. I found that nkx3.2 regulates the expression of two genes with known roles in neural crest migration and specification; pdgfrα and sox10. Further, nkx3.2 functions autonomously in neural crest cells, where knockdown of nkx3.2 causes abnormal migration of neural crest cells in the head. These results show that nkx3.2 regulates genes involved in neural crest migration and in turn supports recruitment of mural cells to mediate vascular stabilization.","abstract_has_math":false,"creators":["Spice, Peter James"],"institution":"Graduate Studies","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Biochemistry and Molecular Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Childs, Sarah"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-01-08","date_published":"2015-01-08","updated_at":"2026-07-24T01:30:44Z","subjects":["Biology--Cell"],"languages":["eng"],"rights":["University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["http://dx.doi.org/10.11575/PRISM/27107"],"render_values":[{"text":"http://dx.doi.org/10.11575/PRISM/27107","href":"http://dx.doi.org/10.11575/PRISM/27107","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/11023/1994","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Childs, Sarah"]},{"key":"dc:creator","label":"Author","values":["Spice, Peter James"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-01-08T16:28:51Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-02-23T08:00:37Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-01-08"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Calgary"]},{"key":"dc:type","label":"Dc Type","values":["master thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry and Molecular Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Calgary"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology--Cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["http://dx.doi.org/10.11575/PRISM/27107"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11023/1994"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Vascular stabilization is the process during vascular development where the immature endothelial cell tube gains structural support through the recruitment and adhesion of perivascular mural cells; pericytes and vascular smooth muscle cells (vSMC). When vessels are not well supported by mural cells hemorrhage can occur. Nkx3.2 deficient zebrafish embryos hemorrhage, have reduced expression of a mural cell marker, and the endothelial layer makes less contact with mural cells compared with controls. I found that nkx3.2 regulates the expression of two genes with known roles in neural crest migration and specification; pdgfrα and sox10. Further, nkx3.2 functions autonomously in neural crest cells, where knockdown of nkx3.2 causes abnormal migration of neural crest cells in the head. These results show that nkx3.2 regulates genes involved in neural crest migration and in turn supports recruitment of mural cells to mediate vascular stabilization."]},{"key":"dc:title","label":"Title","values":["Nkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells"]}]}],"canonical_facts":{"dc:contributor.advisor":["Childs, Sarah"],"dc:creator":["Spice, Peter James"],"dc:date.accessioned":["2015-01-08T16:28:51Z"],"dc:date.available":["2015-02-23T08:00:37Z"],"dc:date.issued":["2015-01-08"],"dc:description.abstract":["Vascular stabilization is the process during vascular development where the immature endothelial cell tube gains structural support through the recruitment and adhesion of perivascular mural cells; pericytes and vascular smooth muscle cells (vSMC). When vessels are not well supported by mural cells hemorrhage can occur. Nkx3.2 deficient zebrafish embryos hemorrhage, have reduced expression of a mural cell marker, and the endothelial layer makes less contact with mural cells compared with controls. I found that nkx3.2 regulates the expression of two genes with known roles in neural crest migration and specification; pdgfrα and sox10. Further, nkx3.2 functions autonomously in neural crest cells, where knockdown of nkx3.2 causes abnormal migration of neural crest cells in the head. These results show that nkx3.2 regulates genes involved in neural crest migration and in turn supports recruitment of mural cells to mediate vascular stabilization."],"dc:identifier.doi":["http://dx.doi.org/10.11575/PRISM/27107"],"dc:identifier.uri":["http://hdl.handle.net/11023/1994"],"dc:language.iso":["eng"],"dc:publisher.institution":["University of Calgary"],"dc:rights":["University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission."],"dc:subject":["Biology--Cell"],"dc:title":["Nkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells"],"dc:type":["master thesis"],"thesis:degree_discipline":["Biochemistry and Molecular Biology"],"thesis:degree_name":["Master of Science (MSc)"],"thesis:institution_name":["University of Calgary"]},"updated_at":"2026-07-24T01:30:44Z"}