Elucidating the Relationship Between Macrophages and Synovial Mesenchymal Progenitor Cell Chondrogenesis

Osteoarthritis (OA) is a complex disease affecting articular cartilage and joint tissues. Macrophages in OA synovium contribute to the inflamed joint environment by releasing inflammatory mediators promoting cartilage loss. What remains unknown is how macrophages interact with synovial mesenchymal progenitor cells (sMPCs) in the joint. sMPCs are able to differentiate into cartilage but for unknown reasons, sMPC chondrogenic capacity is lost in OA. This thesis elucidates the relationship between macrophages and sMPC using synovial explants obtained from OA and normal patients. I found that altered macrophage activity (through cytokine stimulation and/or macrophage depletion) differentially regulates the chondrogenic capacity of sMPCs in a patient-specific manner. Also, the secretory profile of the explant is different between normal and OA patients in response to treatment. Taken together, there is heterogeneity within the responses of the OA patients, highlighting the need for personalized approaches to repair the cartilage within the OA joint.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Medical Science

Grantor dc:publisher.institution

Graduate Studies

Year dc:date.issued

2014

Author dc:creator

• Fichadiya, Akash

Advisor dc:contributor.advisor

• Krawetz, Roman

• Biology--Cell
• Biology--Molecular

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/25868

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:11023/1904