{"id":{"repo_id":"cagliari","oai_identifier":"oai:iris.unica.it:11584/447170"},"canonical_url":"https://search.dev.ndltd.org/etd/cagliari/oai:iris.unica.it:11584/447170","repository":{"repo_id":"cagliari","name":"Università di Cagliari","base_url":"https://iris.unica.it/oai/request"},"display":{"title":"Conjugated linoleic acid isomers anti-neuroinflammatory effects on activated microglial cells","abstract":"Conjugated linoleic acid (CLA) isomers display anti-inflammatory effects within the central nervous system (CNS). This research examined how CLA isomers c9,t11 and t10,c12 influence fatty acid (FA) and N-acylethanolamine (NAE) profiles, along with their relationship with the expression of pro-inflammatory molecules in the BV-2 microglia cell line, which are the resident immune cells in the CNS that play a key role in sustaining neuronal activity and immune balance. BV-2 cells were exposed to 25 μM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 hours, after which they were stimulated with lipopolysaccharide (LPS). Following the treatment, the fatty acid and N-acylethanolamine profiles, as well as the expression of pro-inflammatory molecules, were assessed. Our findings showed that CLA isomers lessen the morphological alterations caused by LPS in BV-2 cells and decrease both the gene expression and protein levels of inflammatory markers. This impact was associated with an increase in acyl-CoA oxidase 1, an important enzyme involved in the anti-inflammatory peroxisomal beta-oxidation pathway that effectively processes CLA isomers. Importantly, t10,c12-CLA markedly inhibited stearoyl-CoA desaturase 1, which influenced the production of monounsaturated fatty acids. The profile of NAEs was significantly modified by CLA isomers, leading to a notable increase in the release of the anti-neuroinflammatory mediator known as docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA). In summary, our research indicates that the anti-neuroinflammatory properties of CLA isomers can be attributed to their effects on fatty acid metabolism and modulation of bioactive fatty acid-derived N-acylethanolamines, emphasizing a potential approach for nutritional interventions in conditions marked by neuroinflammation.","abstract_html":"Conjugated linoleic acid (CLA) isomers display anti-inflammatory effects within the central nervous system (CNS). This research examined how CLA isomers c9,t11 and t10,c12 influence fatty acid (FA) and N-acylethanolamine (NAE) profiles, along with their relationship with the expression of pro-inflammatory molecules in the BV-2 microglia cell line, which are the resident immune cells in the CNS that play a key role in sustaining neuronal activity and immune balance. BV-2 cells were exposed to 25 μM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 hours, after which they were stimulated with lipopolysaccharide (LPS). Following the treatment, the fatty acid and N-acylethanolamine profiles, as well as the expression of pro-inflammatory molecules, were assessed. Our findings showed that CLA isomers lessen the morphological alterations caused by LPS in BV-2 cells and decrease both the gene expression and protein levels of inflammatory markers. This impact was associated with an increase in acyl-CoA oxidase 1, an important enzyme involved in the anti-inflammatory peroxisomal beta-oxidation pathway that effectively processes CLA isomers. Importantly, t10,c12-CLA markedly inhibited stearoyl-CoA desaturase 1, which influenced the production of monounsaturated fatty acids. The profile of NAEs was significantly modified by CLA isomers, leading to a notable increase in the release of the anti-neuroinflammatory mediator known as docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA). In summary, our research indicates that the anti-neuroinflammatory properties of CLA isomers can be attributed to their effects on fatty acid metabolism and modulation of bioactive fatty acid-derived N-acylethanolamines, emphasizing a potential approach for nutritional interventions in conditions marked by neuroinflammation.","abstract_has_math":false,"creators":["PORCEDDA, CLARA"],"institution":"Università degli Studi di Cagliari","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["SOGOS, VALERIA"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-12T00:00:00+01:00","date_published":"2025-02-12T00:00:00+01:00","updated_at":"2026-07-24T01:29:52Z","subjects":["Settore BIO/17 - Istologia"],"languages":["ita"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/11584/447170","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["SOGOS, VALERIA"]},{"key":"dc:creator","label":"Author","values":["PORCEDDA, CLARA"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-12T00:00:00+01:00"]},{"key":"dc:publisher","label":"Institution","values":["Università degli Studi di Cagliari"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Settore BIO/17 - Istologia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ita"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/11584/447170"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Conjugated linoleic acid (CLA) isomers display anti-inflammatory effects within the central nervous system (CNS). This research examined how CLA isomers c9,t11 and t10,c12 influence fatty acid (FA) and N-acylethanolamine (NAE) profiles, along with their relationship with the expression of pro-inflammatory molecules in the BV-2 microglia cell line, which are the resident immune cells in the CNS that play a key role in sustaining neuronal activity and immune balance. BV-2 cells were exposed to 25 μM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 hours, after which they were stimulated with lipopolysaccharide (LPS). Following the treatment, the fatty acid and N-acylethanolamine profiles, as well as the expression of pro-inflammatory molecules, were assessed. Our findings showed that CLA isomers lessen the morphological alterations caused by LPS in BV-2 cells and decrease both the gene expression and protein levels of inflammatory markers. This impact was associated with an increase in acyl-CoA oxidase 1, an important enzyme involved in the anti-inflammatory peroxisomal beta-oxidation pathway that effectively processes CLA isomers. Importantly, t10,c12-CLA markedly inhibited stearoyl-CoA desaturase 1, which influenced the production of monounsaturated fatty acids. The profile of NAEs was significantly modified by CLA isomers, leading to a notable increase in the release of the anti-neuroinflammatory mediator known as docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA). In summary, our research indicates that the anti-neuroinflammatory properties of CLA isomers can be attributed to their effects on fatty acid metabolism and modulation of bioactive fatty acid-derived N-acylethanolamines, emphasizing a potential approach for nutritional interventions in conditions marked by neuroinflammation."]},{"key":"dc:title","label":"Title","values":["Conjugated linoleic acid isomers anti-neuroinflammatory effects on activated microglial cells"]}]}],"canonical_facts":{"dc:contributor":["SOGOS, VALERIA"],"dc:creator":["PORCEDDA, CLARA"],"dc:date":["2025-02-12T00:00:00+01:00"],"dc:description":["Conjugated linoleic acid (CLA) isomers display anti-inflammatory effects within the central nervous system (CNS). This research examined how CLA isomers c9,t11 and t10,c12 influence fatty acid (FA) and N-acylethanolamine (NAE) profiles, along with their relationship with the expression of pro-inflammatory molecules in the BV-2 microglia cell line, which are the resident immune cells in the CNS that play a key role in sustaining neuronal activity and immune balance. BV-2 cells were exposed to 25 μM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 hours, after which they were stimulated with lipopolysaccharide (LPS). Following the treatment, the fatty acid and N-acylethanolamine profiles, as well as the expression of pro-inflammatory molecules, were assessed. Our findings showed that CLA isomers lessen the morphological alterations caused by LPS in BV-2 cells and decrease both the gene expression and protein levels of inflammatory markers. This impact was associated with an increase in acyl-CoA oxidase 1, an important enzyme involved in the anti-inflammatory peroxisomal beta-oxidation pathway that effectively processes CLA isomers. Importantly, t10,c12-CLA markedly inhibited stearoyl-CoA desaturase 1, which influenced the production of monounsaturated fatty acids. The profile of NAEs was significantly modified by CLA isomers, leading to a notable increase in the release of the anti-neuroinflammatory mediator known as docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA). In summary, our research indicates that the anti-neuroinflammatory properties of CLA isomers can be attributed to their effects on fatty acid metabolism and modulation of bioactive fatty acid-derived N-acylethanolamines, emphasizing a potential approach for nutritional interventions in conditions marked by neuroinflammation."],"dc:identifier":["https://hdl.handle.net/11584/447170"],"dc:language":["ita"],"dc:publisher":["Università degli Studi di Cagliari"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:subject":["Settore BIO/17 - Istologia"],"dc:title":["Conjugated linoleic acid isomers anti-neuroinflammatory effects on activated microglial cells"],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T01:29:52Z"}