Photodynamic therapy against multidrug-resistant oral strains of Candida spp
type: CreativeWork
description: Introduction. The advent of Candida ssp. strains that are resistant to different drugs, or MDR strains, highlights the necessity of creating novel antimycotics that are both effective and safe for systemic health. Within this framework, photodynamic therapy (PDT) may be a viable option for treating yeast infections. In this context, the purpose of this work is to evaluate and compare, through a clinical-laboratory multidisciplinary approach, commercially available protocols and new procedures based on new photosensitizers and/or different light radiation sources. In fact, this thesis is aimed at three main objectives: (i) First, describe and compare the clinical effectiveness of the main PDT therapies already used against Candida infection; (ii) conduct a strict investigation of new PDT procedures by using natural compounds as photosensitizers (PT); and (iii) fine-tune new laboratory procedures to evaluate the infection in infected and then PDT-treated cell culture. Methods. A set of in vitro and in vivo studies were performed. The in vitro studies were executed with oral MDR isolates of C. albicans, C. krusei, and C. glabrata, these fungal strains were used from cell suspension in culture liquid medium at their log growth phase. The laboratory procedures were addressed according to EUCAST protocols, which were modified to conform to the PDT treatment, and they could be divided into three main procedures: (i) evaluation with the agar diffusion susceptibility test by Kirby Bauer; (ii) growth curve inhibition and antibiofilm activity through the microplate dilution method; (iii) activity of PDT against a subprosthetic in vitro C. glabrata infection. For these in vitro procedures, a large set of commercial and natural PT as well as different light sources were evaluated. The antimycotic activity were in fact evaluated by using these subsequent commercials formulates: Curcumin/H2O2, Toluidine Blue 1% and Methylene Blue 1%. The natural compounds evaluated as PT were: oli…
abstract: Introduction. The advent of Candida ssp. strains that are resistant to different drugs, or MDR strains, highlights the necessity of creating novel antimycotics that are both effective and safe for systemic health. Within this framework, photodynamic therapy (PDT) may be a viable option for treating yeast infections. In this context, the purpose of this work is to evaluate and compare, through a clinical-laboratory multidisciplinary approach, commercially available protocols and new procedures based on new photosensitizers and/or different light radiation sources. In fact, this thesis is aimed at three main objectives: (i) First, describe and compare the clinical effectiveness of the main PDT therapies already used against Candida infection; (ii) conduct a strict investigation of new PDT procedures by using natural compounds as photosensitizers (PT); and (iii) fine-tune new laboratory procedures to evaluate the infection in infected and then PDT-treated cell culture. Methods. A set of in vitro and in vivo studies were performed. The in vitro studies were executed with oral MDR isolates of C. albicans, C. krusei, and C. glabrata, these fungal strains were used from cell suspension in culture liquid medium at their log growth phase. The laboratory procedures were addressed according to EUCAST protocols, which were modified to conform to the PDT treatment, and they could be divided into three main procedures: (i) evaluation with the agar diffusion susceptibility test by Kirby Bauer; (ii) growth curve inhibition and antibiofilm activity through the microplate dilution method; (iii) activity of PDT against a subprosthetic in vitro C. glabrata infection. For these in vitro procedures, a large set of commercial and natural PT as well as different light sources were evaluated. The antimycotic activity were in fact evaluated by using these subsequent commercials formulates: Curcumin/H2O2, Toluidine Blue 1% and Methylene Blue 1%. The natural compounds evaluated as PT were: oli…
author: CASU, CINZIA
language: eng
keywords: Settore MED/50 - Scienze Tecniche Mediche Applicate
date published: 2024-02-09T00:00:00+01:00
date modified: 2026-07-24T01:29:57Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

PropertyValue
property ID: Handle
value: https://hdl.handle.net/11584/394165