Modulatory effect of EVOO polyphenols and their metabolites on inflammatory response in Adenocarcinoma Colon Cells (Caco-2) and Human Umbilical Vein EC (HUVEC)
type: CreativeWork
description: Mediterranean diet is considered a model of healthy nutrition, able to prevent and/or slow down the progression of the most common degenerative diseases, such as Cardiovascular Disease (CVD) and Inflammatory Bowel Disease (IBD), characterized by a chronic inflammation state that alters structure and functionality of the endothelial and intestinal barrier. EVOO(EVOO), the main lipid source in the Mediterranean diet, is rich in bioactive compounds mainly hydroxytyrosol (HT) and tyrosol (Tyr), that have been shown to possess antimicrobial, antioxidant and anti-inflammatory properties. However, it is well known that free forms of phenolic compounds ingested with food are largely metabolized in the intestine and liver and their concentrations after ingestion are too low to explain the biological effects observed in vivo and in vitro models. In particular, HT and Tyr undergo glucuronidation and sulfonation, and their sulphate and glucuronide metabolites are the prevalent forms found in human plasma and urine where they reach relevant concentrations compatible with biological activity. In this context, the aim of the present research project was to evaluate the protective effect of the main phenolic compounds of EVOO, HT and Tyr, and their sulphate and glucuronide metabolites, on the inflammatory response at intestinal level (in Caco-2 cell monolayer) and endothelial level (in HUVEC cell monolayer), using pro-inflammatory stimuli, such as LPS and/or a hyperglycemia (HG) condition. Their modulatory action was evaluated focusing on the alteration of tight junctions (TJ) and the activation of cellular pathways, as mitogen-activated protein kinases (MAPK) and NLRP3 inflammasome, which are linked to chronic inflammatory diseases. The alteration of epithelial/endothelial barrier in Caco-2 and HUVEC monolayers, treated with LPS or HG alone or together with EVOO phenolic compounds and their sulphate and glucuronide metabolites, was evaluated through cell permeability tests (TEER, …
abstract: Mediterranean diet is considered a model of healthy nutrition, able to prevent and/or slow down the progression of the most common degenerative diseases, such as Cardiovascular Disease (CVD) and Inflammatory Bowel Disease (IBD), characterized by a chronic inflammation state that alters structure and functionality of the endothelial and intestinal barrier. EVOO(EVOO), the main lipid source in the Mediterranean diet, is rich in bioactive compounds mainly hydroxytyrosol (HT) and tyrosol (Tyr), that have been shown to possess antimicrobial, antioxidant and anti-inflammatory properties. However, it is well known that free forms of phenolic compounds ingested with food are largely metabolized in the intestine and liver and their concentrations after ingestion are too low to explain the biological effects observed in vivo and in vitro models. In particular, HT and Tyr undergo glucuronidation and sulfonation, and their sulphate and glucuronide metabolites are the prevalent forms found in human plasma and urine where they reach relevant concentrations compatible with biological activity. In this context, the aim of the present research project was to evaluate the protective effect of the main phenolic compounds of EVOO, HT and Tyr, and their sulphate and glucuronide metabolites, on the inflammatory response at intestinal level (in Caco-2 cell monolayer) and endothelial level (in HUVEC cell monolayer), using pro-inflammatory stimuli, such as LPS and/or a hyperglycemia (HG) condition. Their modulatory action was evaluated focusing on the alteration of tight junctions (TJ) and the activation of cellular pathways, as mitogen-activated protein kinases (MAPK) and NLRP3 inflammasome, which are linked to chronic inflammatory diseases. The alteration of epithelial/endothelial barrier in Caco-2 and HUVEC monolayers, treated with LPS or HG alone or together with EVOO phenolic compounds and their sulphate and glucuronide metabolites, was evaluated through cell permeability tests (TEER, …
author: ZODIO, SONIA
language: eng
keywords: Settore MED/04 - Patologia Generale
date published: 2023-04-18T00:00:00+02:00
date modified: 2026-07-24T01:29:52Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

PropertyValue
property ID: Handle
value: https://hdl.handle.net/11584/359586