Il trattamento neonatale con estradiolo modifica l'espressione e la funzione dei recettori GABAA e la sensibilità allo stress nel ratto adulto
type: CreativeWork
description: Exposure of female rats to estradiol during the perinatal period affects brain sexual differentiation and induces a long-lasting dysregulation of the gonadal axis with reduced progesterone secretion, associated with persistent reductions in the cerebrocortical, hypothalamic and plasma concentrations of its metabolite, allopregnanolone. Given that allopregnanolone is a potent positive modulator of GABAA receptor (GABAAR) expression and function, it has been evaluated whether neonatal estradiol treatment affects, during adulthood, GABAAR expression and function in the hippocampus, an area that exhibits the two components, tonic and phasic, of the inhibitory GABAergic transmission. Neonatal estradiol administration to female rats induced a selective reduction in hippocampal allopregnanolone levels in adult animals, associated with increased expression of extrasynaptic α4/δ subunit-containing GABAARs and tonic currents. Moreover, the same treatment altered the expression of synaptic receptors containing α1/α4/γ2 subunits as well as phasic currents. The changes in the expression and function of GABAARs, induced by neonatal estradiol treatment, may not be related to the fluctuations in allopregnanolone concentrations, given that vehicle-treated rats in diestrus 1, which have opposite neurosteroid levels than estradiol-treated rats, show the same functional changes in GABAARs as estradiol-treated rats. Allopregnanolone plays an important role in hypothalamic-pituitary-adrenal (HPA) homeostasis and in the stress response. Thus, it has been examined whether neonatal exposure to estradiol was associated with altered HPA axis responsiveness and sensitivity to stress in the adulthood. Neonatal estradiol treatment induced a marked increase in brain and plasma allopregnanolone levels following foot-shock stress, an effect not observed in control animals. Moreover, acute stress increased brain and plasma corticosterone levels to a similar extent in vehicle- and estradiol-treated r…
abstract: Exposure of female rats to estradiol during the perinatal period affects brain sexual differentiation and induces a long-lasting dysregulation of the gonadal axis with reduced progesterone secretion, associated with persistent reductions in the cerebrocortical, hypothalamic and plasma concentrations of its metabolite, allopregnanolone. Given that allopregnanolone is a potent positive modulator of GABAA receptor (GABAAR) expression and function, it has been evaluated whether neonatal estradiol treatment affects, during adulthood, GABAAR expression and function in the hippocampus, an area that exhibits the two components, tonic and phasic, of the inhibitory GABAergic transmission. Neonatal estradiol administration to female rats induced a selective reduction in hippocampal allopregnanolone levels in adult animals, associated with increased expression of extrasynaptic α4/δ subunit-containing GABAARs and tonic currents. Moreover, the same treatment altered the expression of synaptic receptors containing α1/α4/γ2 subunits as well as phasic currents. The changes in the expression and function of GABAARs, induced by neonatal estradiol treatment, may not be related to the fluctuations in allopregnanolone concentrations, given that vehicle-treated rats in diestrus 1, which have opposite neurosteroid levels than estradiol-treated rats, show the same functional changes in GABAARs as estradiol-treated rats. Allopregnanolone plays an important role in hypothalamic-pituitary-adrenal (HPA) homeostasis and in the stress response. Thus, it has been examined whether neonatal exposure to estradiol was associated with altered HPA axis responsiveness and sensitivity to stress in the adulthood. Neonatal estradiol treatment induced a marked increase in brain and plasma allopregnanolone levels following foot-shock stress, an effect not observed in control animals. Moreover, acute stress increased brain and plasma corticosterone levels to a similar extent in vehicle- and estradiol-treated r…
author: LOCCI, ANDREA
language: ita
keywords: allopregnanolone, estradiol, estradiolo, receptors, recettori GABAA, stress, Settore BIO/14 - Farmacologia
date published: 2016-03-08
date modified: 2026-07-24T01:30:12Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

PropertyValue
property ID: Handle
value: http://hdl.handle.net/11584/266889