VGF e stress ossidativo: alterazioni cellulari e modulazione nella Sclerosi Laterale Amiotrofica
type: CreativeWork
description: The VGF gene encodes a protein precursor (617/615 AA rat/man, respectively), which is processed in vivo to various low MW peptides with different biologic activities. These include attenuation of excytotoxic injury on spinal motor neurons from G93A-SOD-1 mice, upon overexpression of the VGF precursor, or an anti-apoptotic action of the VGF peptide TLQP-21 on cerebellar granules. Recently, the C3a and gC1q have been identified as TLQP-21 receptors. Using the mouse motoneuronal cell line NSC-34 and the primary human fibroblast cell colture, we studied the expression and modulation of certain VGF peptides (VGFp) in ALS and oxidative stress and their possible neuroprotective effect. Oxidative stress was induced with Na Arsenite (SA: 0.5mM, 30/60 min) while the MTT test was used to assess cell viability. Antibodies used (for either ICC or ELISA) were specific for: the N/C-terminus of the VGF precursor, NERP-1, rat PGH, NAPPE-19 and TLQP-21, and for the C3a/gC1q TLQP receptors. Both antibody receptors were also used for ICC and western blot analysis. In NSC-34, almost all VGFp were selectively localized in the growth cones, perinuclear region, and/or in a specific paranuclear region. Upon oxidative stress, most cells showed a rounded morphology, with loss of growth cones, while almost all VGFp were found mainly in a paranuclear region compatible with the Golgi apparatus. In ELISA, untreated cellular extracts showed the presence of the majority VGFp with a significant reduction in TLQP and NERP-1 levels versus untreated cells (58% and 65% of reduction, respectively) after SA treatment, while the culture medium showed a marginal increase of the same peptides. Cell viability studies showed a significant protective action of TLQP-21 on stressed neurons (SA alone: 74.7% 0.08, SA and TLQP-21 together: 82.1% 0.08 of untreated controls, p0.018), with no detectable effect for NERP-1. Results from immunocytochemistry showed the presence in NSC-34 of gC1q receptor only, confirmed…
abstract: The VGF gene encodes a protein precursor (617/615 AA rat/man, respectively), which is processed in vivo to various low MW peptides with different biologic activities. These include attenuation of excytotoxic injury on spinal motor neurons from G93A-SOD-1 mice, upon overexpression of the VGF precursor, or an anti-apoptotic action of the VGF peptide TLQP-21 on cerebellar granules. Recently, the C3a and gC1q have been identified as TLQP-21 receptors. Using the mouse motoneuronal cell line NSC-34 and the primary human fibroblast cell colture, we studied the expression and modulation of certain VGF peptides (VGFp) in ALS and oxidative stress and their possible neuroprotective effect. Oxidative stress was induced with Na Arsenite (SA: 0.5mM, 30/60 min) while the MTT test was used to assess cell viability. Antibodies used (for either ICC or ELISA) were specific for: the N/C-terminus of the VGF precursor, NERP-1, rat PGH, NAPPE-19 and TLQP-21, and for the C3a/gC1q TLQP receptors. Both antibody receptors were also used for ICC and western blot analysis. In NSC-34, almost all VGFp were selectively localized in the growth cones, perinuclear region, and/or in a specific paranuclear region. Upon oxidative stress, most cells showed a rounded morphology, with loss of growth cones, while almost all VGFp were found mainly in a paranuclear region compatible with the Golgi apparatus. In ELISA, untreated cellular extracts showed the presence of the majority VGFp with a significant reduction in TLQP and NERP-1 levels versus untreated cells (58% and 65% of reduction, respectively) after SA treatment, while the culture medium showed a marginal increase of the same peptides. Cell viability studies showed a significant protective action of TLQP-21 on stressed neurons (SA alone: 74.7% 0.08, SA and TLQP-21 together: 82.1% 0.08 of untreated controls, p0.018), with no detectable effect for NERP-1. Results from immunocytochemistry showed the presence in NSC-34 of gC1q receptor only, confirmed…
author: PILLERI, ROBERTA
language: ita
keywords: ALS, SLA, TLQP-21, VGF, VGF Peptidi, VGF peptides, oxidative stress, stress ossidativo, Settore BIO/16 - Anatomia Umana
date published: 2016-03-15
date modified: 2026-07-24T01:30:12Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

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property ID: Handle
value: http://hdl.handle.net/11584/266645