Sequenziamento e Analisi Molecolare di Varianti Alleliche del Gene PRB1
type: CreativeWork
description: The aim of this work was the molecular characterization of gene PRB1 in three subjects with different salivary proteome profile. The PRB1 gene encodes a member of the heterogeneous family of Basic Proline rich Proteins (PRB), produced by the parotid gland and secreted in human saliva. The protein polymorphism resulting from post-translational modifications of the pre-protein PRB1, are further complicated by the gene allelic variants. Those variants involves the third exon of the gene, and are related to the presence or absence of a tandem repeat sequence 183 bp long. That tandem repeat may originate three different allelic forms, defined small, medium or large depending on the number of repeats inside the third exon. The information reported in the literature about the class of PRP genes, and in particular PRB1 are, from the molecular point of view, fragmentary and difficult to reconstruct. In fact, the literature on this topic covers a time span ranging from the seventies (Azen & Oppenheim, 1973), when the associated peptides have been characterized for the first time, up to the end of the nineties (Stubbs et al., 1998). Since the eighties, there have been various attempts to characterize the PRB1 allelic variants, based on the resulting peptides. This approach has greatly complicated the classification of the gene, but also of his allelic variants and the deriving proteins (Maeda et al., 1985 ; Lyons et al., 1988a, b; Azen et al., 1993). Today we know that from PRB1, considering its allelic and splicing variants, are expressed six different proteins (Marconi et al., 2015). However, amongst the three allelic variants of this gene, the only of which we have the complete nucleotide sequence is the medium. Conversely, for the small and large we have so far only partial sequences, referring only to the third exon. Among the objectives of this study, there was the reconstruction of the complete sequences of the small and large variants, of which there have been previous…
abstract: The aim of this work was the molecular characterization of gene PRB1 in three subjects with different salivary proteome profile. The PRB1 gene encodes a member of the heterogeneous family of Basic Proline rich Proteins (PRB), produced by the parotid gland and secreted in human saliva. The protein polymorphism resulting from post-translational modifications of the pre-protein PRB1, are further complicated by the gene allelic variants. Those variants involves the third exon of the gene, and are related to the presence or absence of a tandem repeat sequence 183 bp long. That tandem repeat may originate three different allelic forms, defined small, medium or large depending on the number of repeats inside the third exon. The information reported in the literature about the class of PRP genes, and in particular PRB1 are, from the molecular point of view, fragmentary and difficult to reconstruct. In fact, the literature on this topic covers a time span ranging from the seventies (Azen & Oppenheim, 1973), when the associated peptides have been characterized for the first time, up to the end of the nineties (Stubbs et al., 1998). Since the eighties, there have been various attempts to characterize the PRB1 allelic variants, based on the resulting peptides. This approach has greatly complicated the classification of the gene, but also of his allelic variants and the deriving proteins (Maeda et al., 1985 ; Lyons et al., 1988a, b; Azen et al., 1993). Today we know that from PRB1, considering its allelic and splicing variants, are expressed six different proteins (Marconi et al., 2015). However, amongst the three allelic variants of this gene, the only of which we have the complete nucleotide sequence is the medium. Conversely, for the small and large we have so far only partial sequences, referring only to the third exon. Among the objectives of this study, there was the reconstruction of the complete sequences of the small and large variants, of which there have been previous…
author: ORRU', ROBERTO
language: eng
keywords: DNA, PCR, PRB1, primer walking, proline-rich proteins, proteoma salivare, Settore BIO/10 - Biochimica
date published: 2016-03-11
date modified: 2026-07-24T01:30:09Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

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property ID: Handle
value: http://hdl.handle.net/11584/266626