Caratterizzazione dimensionale e morfologica di nanoparticelle lipidiche contenenti molecole farmacologicamente attive
type: CreativeWork
description: With the advent of nanotechnology, great interest is being dedicated to the interactions of nanomaterials/nanoparticles with human beings. All the interactions occurring due to an intentional exposure are investigated by a new research field named Nanomedicine. As this name may suggest, the use of drug delivery systems, sized in the nanometric scale, may favor overcoming anatomical barriers to reach, passively or by a targeting design, the body area to be pharmacologically treated. In this thesis, we focused our attention to the morphological and dimensional characterization of pharmaceutical lipidic nanoparticles (NP). These carriers were selected because we hypothesized that they could be good candidates for potent but lipophilic drugs that cannot be systemically administered with conventional formulations (e.g., solutions, dispersions). In addition, we focused on their preparation and characterization to evaluate potential therapeutic applications based on shape and size. Specifically, we prepared two types of NP named nanocapsules (NC) and nanoemulsions (NE); the former differs from the latter for a polysaccharidic capsule. Several drugs (ibuprofen and its sodium salt, transretinoic acid, paclitaxel, AZL 6 and 38) and two fluorescently-labeled lipids (i.e.,FITC-PE, NBD) have been entrapped within both NP. We found that lipophilic molecules were completely encapsulated in both NP. In contrast, in the case of the hydrophilic drug, the polysaccharidic capsule causes ibuprofen sodium salt to be entrapped with a greater efficiency in NC than NE (i.e. 92.6 % IE in NC vs. 20.4 % IE in NE). Morphological analysis has been carried out on NP, that were not subjected to a purification treatment, by TEM, and in a few cases also by Cryo-TEM. Although the sample drying step showed to be very critical in producing artifacts, we arrived to the conclusion that particles were spherical. Dimensional analysis has been carried out with TEM and DLS on NP subjected or not-subjected to…
abstract: With the advent of nanotechnology, great interest is being dedicated to the interactions of nanomaterials/nanoparticles with human beings. All the interactions occurring due to an intentional exposure are investigated by a new research field named Nanomedicine. As this name may suggest, the use of drug delivery systems, sized in the nanometric scale, may favor overcoming anatomical barriers to reach, passively or by a targeting design, the body area to be pharmacologically treated. In this thesis, we focused our attention to the morphological and dimensional characterization of pharmaceutical lipidic nanoparticles (NP). These carriers were selected because we hypothesized that they could be good candidates for potent but lipophilic drugs that cannot be systemically administered with conventional formulations (e.g., solutions, dispersions). In addition, we focused on their preparation and characterization to evaluate potential therapeutic applications based on shape and size. Specifically, we prepared two types of NP named nanocapsules (NC) and nanoemulsions (NE); the former differs from the latter for a polysaccharidic capsule. Several drugs (ibuprofen and its sodium salt, transretinoic acid, paclitaxel, AZL 6 and 38) and two fluorescently-labeled lipids (i.e.,FITC-PE, NBD) have been entrapped within both NP. We found that lipophilic molecules were completely encapsulated in both NP. In contrast, in the case of the hydrophilic drug, the polysaccharidic capsule causes ibuprofen sodium salt to be entrapped with a greater efficiency in NC than NE (i.e. 92.6 % IE in NC vs. 20.4 % IE in NE). Morphological analysis has been carried out on NP, that were not subjected to a purification treatment, by TEM, and in a few cases also by Cryo-TEM. Although the sample drying step showed to be very critical in producing artifacts, we arrived to the conclusion that particles were spherical. Dimensional analysis has been carried out with TEM and DLS on NP subjected or not-subjected to…
author: MANCA, MARIANNA
language: ita
keywords: Caratterizzazione nanoparticelle lipidiche con farmaci, characterization nanoparticles with drug, Settore BIO/16 - Anatomia Umana
date published: 2012-03-02
date modified: 2026-07-24T01:30:05Z
provider: Università di Cagliari
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Università degli Studi di Cagliari

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property ID: Handle
value: http://hdl.handle.net/11584/266089