Association between the gut microbiota profile, fat oxidation capacity during exercise, and leptin sensitivity in patients with type 2 diabetes mellitus: The Edugution Project
type: CreativeWork
description: Type 2 diabetes (T2D) is a cardiometabolic disorder characterized by chronic hyperglycemia due to insulin resistance or insufficient secretion. Individuals with T2D experience difficulties in the oxidation of fats and glucose, a phenomenon known as metabolic inflexibility, which negatively impacts their body composition and overall metabolic health. As maximal fat oxidation (MFO) is typically assessed with protocols requiring two separate sessions or estimations from non-specific tests, a one-day test has been validated to assess MFO, its intensity (FATmax), and maximal oxygen consumption (VO2max) in individuals with T2D (Study I). This study, which established the validity and reliability of the test, involved 20 adults with pre-diabetes or T2D (10 women). Participants underwent a single-day MFO-VO2max protocol, followed by the traditional VO2max test on a cycle ergometer 4 7 days later, with both assessments performed using indirect calorimetry. The single-day test commenced with the MFO test, involving 15 W per 3 min increments until the respiratory exchange rate reached 1.0. After a 5-minute (min) rest, the VO2max test was conducted, starting at the last load and increasing by 15 Wmin-1 until voluntary exhaustion. The traditional VO2max test involved a 15 Wmin-1n incremental protocol until voluntary exhaustion, followed by a verification test at 110% of the previous maximum load. The Bland-Altman method was employed to determine the limits of agreement between VO2max-A and VO2max-B. The intraclass correlation coefficient (ICC) with a 95% confidence interval was used to assess the reliability of VO2max. The standard error of measurement (SEM) was calculated to assess the measurement error of the VO2max-A test. Sensitivity was evaluated using the minimal detectable change at a 95% confidence interval (MDC95). The findings indicate that the single-day test is a valid and reliable method for assessing these health outcomes in this population, enhancing clinical appl…
abstract: Type 2 diabetes (T2D) is a cardiometabolic disorder characterized by chronic hyperglycemia due to insulin resistance or insufficient secretion. Individuals with T2D experience difficulties in the oxidation of fats and glucose, a phenomenon known as metabolic inflexibility, which negatively impacts their body composition and overall metabolic health. As maximal fat oxidation (MFO) is typically assessed with protocols requiring two separate sessions or estimations from non-specific tests, a one-day test has been validated to assess MFO, its intensity (FATmax), and maximal oxygen consumption (VO2max) in individuals with T2D (Study I). This study, which established the validity and reliability of the test, involved 20 adults with pre-diabetes or T2D (10 women). Participants underwent a single-day MFO-VO2max protocol, followed by the traditional VO2max test on a cycle ergometer 4 7 days later, with both assessments performed using indirect calorimetry. The single-day test commenced with the MFO test, involving 15 W per 3 min increments until the respiratory exchange rate reached 1.0. After a 5-minute (min) rest, the VO2max test was conducted, starting at the last load and increasing by 15 Wmin-1 until voluntary exhaustion. The traditional VO2max test involved a 15 Wmin-1n incremental protocol until voluntary exhaustion, followed by a verification test at 110% of the previous maximum load. The Bland-Altman method was employed to determine the limits of agreement between VO2max-A and VO2max-B. The intraclass correlation coefficient (ICC) with a 95% confidence interval was used to assess the reliability of VO2max. The standard error of measurement (SEM) was calculated to assess the measurement error of the VO2max-A test. Sensitivity was evaluated using the minimal detectable change at a 95% confidence interval (MDC95). The findings indicate that the single-day test is a valid and reliable method for assessing these health outcomes in this population, enhancing clinical appl…
author: Marín Galindo, Alberto
language: eng
license: http://creativecommons.org/licenses/by-nc-nd/4.0/
date published: 2025-06-20
date modified: 2026-07-24T01:29:47Z
provider: Universidad de Cadiz
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Universidad de Cadiz

PropertyValue
property ID: Handle
value: http://hdl.handle.net/10498/37967