Identification of genetic markers associated with growth and morphology quality in senegalese sole (solea senegalensis) to boost aquaculture production
type: CreativeWork
description: The Senegalese sole (Solea senegalensis) is one of the most valuable flatfish in aquaculture in Southern Europe and although the production has grown exponentially in the last decade, reproduction success, health status, and the improvement of growth rates and morphology quality still remain as important limitations for industrial exploitation. The development of breeding programs is a fundamental tool to solve these problems but requires estimate genetic components of economically valuable traits such as those growth and morphology related under industrial conditions. By other side, the advances in genomics provide new highly powerful analysis tools to determine more accurately genetic components productive traits. In this thesis, new genomic tools and molecular markers as well as the genetic components of growth and morphology quality traits have been developed in sole. For this purpose, firstly, a highdensity SNP genetic map and a de novo sole genome assembly were generated. Later, genetic and physical maps were anchored and integrated into 21 linkage groups (SseLGs) corresponding to the expected number of chromosomes of this species. Genetic map was bigger in female than male (1.49) observing also a different recombination rate landscape between sexes. The integrated physical map obtained was used for an association study to identify sex-linked markers. Seven families were analyzed using ddRAD and 30 significant sex-associated SNP markers located onto SseLG18 were identified. Searching for candidate genes for sex determination identified the follicle stimulating hormone receptor (fshr) that it was located within a hot recombination region although with an incomplete penetrance. In addition to SNP markers, genome information was used for searching and identifying SSR markers. Hence, 108 new SSR markers distributed throughout the genome were identified. They were structured in 13 PCR Multiplex assays (with up to 10‐plex) and the amplification conditions were optim…
abstract: The Senegalese sole (Solea senegalensis) is one of the most valuable flatfish in aquaculture in Southern Europe and although the production has grown exponentially in the last decade, reproduction success, health status, and the improvement of growth rates and morphology quality still remain as important limitations for industrial exploitation. The development of breeding programs is a fundamental tool to solve these problems but requires estimate genetic components of economically valuable traits such as those growth and morphology related under industrial conditions. By other side, the advances in genomics provide new highly powerful analysis tools to determine more accurately genetic components productive traits. In this thesis, new genomic tools and molecular markers as well as the genetic components of growth and morphology quality traits have been developed in sole. For this purpose, firstly, a highdensity SNP genetic map and a de novo sole genome assembly were generated. Later, genetic and physical maps were anchored and integrated into 21 linkage groups (SseLGs) corresponding to the expected number of chromosomes of this species. Genetic map was bigger in female than male (1.49) observing also a different recombination rate landscape between sexes. The integrated physical map obtained was used for an association study to identify sex-linked markers. Seven families were analyzed using ddRAD and 30 significant sex-associated SNP markers located onto SseLG18 were identified. Searching for candidate genes for sex determination identified the follicle stimulating hormone receptor (fshr) that it was located within a hot recombination region although with an incomplete penetrance. In addition to SNP markers, genome information was used for searching and identifying SSR markers. Hence, 108 new SSR markers distributed throughout the genome were identified. They were structured in 13 PCR Multiplex assays (with up to 10‐plex) and the amplification conditions were optim…
author: Guerrero Cózar, Israel
language: eng
keywords: Senegalese sole, genetic components, aquaculture
license: http://creativecommons.org/licenses/by-nc-nd/4.0/
date published: 2021
date modified: 2026-07-24T01:29:38Z
provider: Universidad de Cadiz
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Universidad de Cadiz

PropertyValue
property ID: Handle
value: http://hdl.handle.net/10498/29005