{"id":{"repo_id":"byu","oai_identifier":"oai:scholarsarchive.byu.edu:etd-1371"},"canonical_url":"https://search.dev.ndltd.org/etd/byu/oai:scholarsarchive.byu.edu:etd-1371","repository":{"repo_id":"byu","name":"Brigham Young University","base_url":"https://scholarsarchive.byu.edu/do/oai/"},"display":{"title":"Membrane-Based Protein Preconcentration Microfluidic Devices","abstract":"Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced fluorescence measurement. An integrated system that combines analyte preconcentration with microchip CE has also been developed using two different fabrication methods: polymerization and solvent bonding. In both approaches, microchannels within the PMMA substrates were interfaced with an ion-permeable hydrogel. When an electrical potential was applied along the channel, greater than 10,000-fold preconcentration was achieved for R-phycoerythrin. Concentrated protein samples were also injected and separated in these integrated microdevices. Membrane-based protein preconcentration devices can significantly increase the concentration range of biological samples that can be analyzed by microchip CE.","abstract_html":"Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced fluorescence measurement. An integrated system that combines analyte preconcentration with microchip CE has also been developed using two different fabrication methods: polymerization and solvent bonding. In both approaches, microchannels within the PMMA substrates were interfaced with an ion-permeable hydrogel. When an electrical potential was applied along the channel, greater than 10,000-fold preconcentration was achieved for R-phycoerythrin. Concentrated protein samples were also injected and separated in these integrated microdevices. Membrane-based protein preconcentration devices can significantly increase the concentration range of biological samples that can be analyzed by microchip CE.","abstract_has_math":false,"creators":["Li, Yi"],"institution":"Brigham Young University - Provo","degree_name":"MS","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T01:27:43Z","subjects":["Membrane","Microfluidic devices","Protein","Preconcentration","Capillary electrophoresis","Biochemistry","Chemistry"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsarchive.byu.edu/etd/372","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Li, Yi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2006-03-16T08:00:00Z"]},{"key":"dc:publisher","label":"Institution","values":["Brigham Young University - Provo"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Membrane","Microfluidic devices","Protein","Preconcentration","Capillary electrophoresis","Biochemistry","Chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsarchive.byu.edu/etd/372","https://scholarsarchive.byu.edu/context/etd/article/1371/viewcontent/ETD_CISOPTR_592.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Physical and Mathematical Sciences; Chemistry and Biochemistry"]},{"key":"dc:description.abstract","label":"Abstract","values":["Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced fluorescence measurement. An integrated system that combines analyte preconcentration with microchip CE has also been developed using two different fabrication methods: polymerization and solvent bonding. In both approaches, microchannels within the PMMA substrates were interfaced with an ion-permeable hydrogel. When an electrical potential was applied along the channel, greater than 10,000-fold preconcentration was achieved for R-phycoerythrin. Concentrated protein samples were also injected and separated in these integrated microdevices. Membrane-based protein preconcentration devices can significantly increase the concentration range of biological samples that can be analyzed by microchip CE."]},{"key":"dc:format","label":"Dc Format","values":["application:pdf"]},{"key":"dc:source","label":"Dc Source","values":["Brigham Young University - Provo"]},{"key":"dc:title","label":"Title","values":["Membrane-Based Protein Preconcentration Microfluidic Devices"]}]}],"canonical_facts":{"dc:creator":["Li, Yi"],"dc:date":["2006-03-16T08:00:00Z"],"dc:description":["Physical and Mathematical Sciences; Chemistry and Biochemistry"],"dc:description.abstract":["Interest in microchip capillary electrophoresis (CE) is growing due to the rapid analysis times provided and small sample input requirements. However, higher-concentration samples are typically needed because of the small (~pL) detection volumes in these devices. I have made membrane-based protein preconcentration systems in capillary and microchip designs to increase the detectability of low-concentration biological samples. A photopolymerized ion-permeable membrane interfaced with a microchannel in poly(methyl methacrylate) (PMMA) formed the preconcentrator. When a voltage was applied between the sample reservoir and the ionically conductive membrane in a capillary-based system, R-phycoerythrin was concentrated more than 1,000 fold, as determined by laser-induced fluorescence measurement. An integrated system that combines analyte preconcentration with microchip CE has also been developed using two different fabrication methods: polymerization and solvent bonding. In both approaches, microchannels within the PMMA substrates were interfaced with an ion-permeable hydrogel. When an electrical potential was applied along the channel, greater than 10,000-fold preconcentration was achieved for R-phycoerythrin. Concentrated protein samples were also injected and separated in these integrated microdevices. Membrane-based protein preconcentration devices can significantly increase the concentration range of biological samples that can be analyzed by microchip CE."],"dc:format":["application:pdf"],"dc:identifier":["https://scholarsarchive.byu.edu/etd/372","https://scholarsarchive.byu.edu/context/etd/article/1371/viewcontent/ETD_CISOPTR_592.pdf"],"dc:language":["English"],"dc:publisher":["Brigham Young University - Provo"],"dc:source":["Brigham Young University - Provo"],"dc:subject":["Membrane","Microfluidic devices","Protein","Preconcentration","Capillary electrophoresis","Biochemistry","Chemistry"],"dc:title":["Membrane-Based Protein Preconcentration Microfluidic Devices"],"dc:type":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T01:27:43Z"}