{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86802"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86802","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Role of Trace Amine-Associated Receptor 1 in Nicotine Withdrawal","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Huang, Yufei; 0000-0001-5432-8181"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Li, Jun-Xu","Pharmacology and Toxicology"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-25T23:22:54Z","date_published":"2025-02-25T23:22:54Z","updated_at":"2026-07-27T19:05:37Z","subjects":["pharmacology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86802","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Li, Jun-Xu","Pharmacology and Toxicology"]},{"key":"dc:creator","label":"Author","values":["Huang, Yufei; 0000-0001-5432-8181"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-25T23:22:54Z","2020","2020-07-03 18:04:53"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["pharmacology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86802"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Nicotine addiction remains a major health and social problem. Our previous study showed that trace amine-associated receptor 1(TAAR1), a target for the modulation of central dopaminergic activity, remarkably attenuates several abuse-related behaviors of nicotine in rats. However, whether TAAR1 plays a role in nicotine withdrawal remains unknown. Here, we examined the effects of TAAR1 in nicotine withdrawal using the partial agonist RO5263397. Rats underwent short-access (ShA) and long-access (LgA) nicotine self-administration training, respectively. Mecamylamine-precipitated withdrawal symptoms and elevated plus maze (EPM) test were performed to determine somatic withdrawal signs and anxiety-like behavior three days after the last training session. Von Frey filament tests were observed both at 1h and 72h after the last training session. We found that nicotine ShA rats achieved more infusions than the saline group, demonstrating the reinforcing effect of nicotine. Moreover, rats in LgA group obtained more nicotine than ShA group. ShA rats did not show significant withdrawal signs or anxiety-like behavior compared with saline group. In contrast, the LgA rats had more somatic withdrawal signs and spent more time in the open arm in the EPM test. More importantly, RO5263397 significantly reduced the somatic signs and anxiety-like behavior in LgA rats. There was no difference in the paw withdrawal threshold among all groups 1 hour after the training. However, LgA rats showed decreased paw withdrawal threshold compared to saline and ShA rats 72 hours after the last training, and RO5263397 completely reversed the hypersensitivity in LgA rats. These results strongly support that TAAR1 plays an important role in nicotine withdrawal.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of Trace Amine-Associated Receptor 1 in Nicotine Withdrawal"]}]}],"canonical_facts":{"dc:contributor":["Li, Jun-Xu","Pharmacology and Toxicology"],"dc:creator":["Huang, Yufei; 0000-0001-5432-8181"],"dc:date":["2025-02-25T23:22:54Z","2020","2020-07-03 18:04:53"],"dc:description":["M.S.","Nicotine addiction remains a major health and social problem. Our previous study showed that trace amine-associated receptor 1(TAAR1), a target for the modulation of central dopaminergic activity, remarkably attenuates several abuse-related behaviors of nicotine in rats. However, whether TAAR1 plays a role in nicotine withdrawal remains unknown. Here, we examined the effects of TAAR1 in nicotine withdrawal using the partial agonist RO5263397. Rats underwent short-access (ShA) and long-access (LgA) nicotine self-administration training, respectively. Mecamylamine-precipitated withdrawal symptoms and elevated plus maze (EPM) test were performed to determine somatic withdrawal signs and anxiety-like behavior three days after the last training session. Von Frey filament tests were observed both at 1h and 72h after the last training session. We found that nicotine ShA rats achieved more infusions than the saline group, demonstrating the reinforcing effect of nicotine. Moreover, rats in LgA group obtained more nicotine than ShA group. ShA rats did not show significant withdrawal signs or anxiety-like behavior compared with saline group. In contrast, the LgA rats had more somatic withdrawal signs and spent more time in the open arm in the EPM test. More importantly, RO5263397 significantly reduced the somatic signs and anxiety-like behavior in LgA rats. There was no difference in the paw withdrawal threshold among all groups 1 hour after the training. However, LgA rats showed decreased paw withdrawal threshold compared to saline and ShA rats 72 hours after the last training, and RO5263397 completely reversed the hypersensitivity in LgA rats. These results strongly support that TAAR1 plays an important role in nicotine withdrawal.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86802"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["pharmacology"],"dc:title":["The Role of Trace Amine-Associated Receptor 1 in Nicotine Withdrawal"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:37Z"}