{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86751"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86751","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Evaluation of Viable Bacterial Retention on Sutures After Three Different Mouthrinse Treatments","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Gempita, Gema; 0000-0003-0621-5419"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Meyer, Anne","Biomaterials"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T21:44:43Z","date_published":"2025-02-21T21:44:43Z","updated_at":"2026-07-27T19:05:37Z","subjects":["dentistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86751","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Meyer, Anne","Biomaterials"]},{"key":"dc:creator","label":"Author","values":["Gempita, Gema; 0000-0003-0621-5419"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T21:44:43Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86751"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","In the oral cavity, sutures are continuously bathed in saliva containing approximately 7.5x108 microorganisms/ml which can attach to the suture surface and lead to prolonged infection. Chlorhexidine mouthrinse is considered to be the gold standard for antibacterial treatment in dentistry. However, it can have several negative side-effects in the mouth, such as discoloration on teeth surfaces and oral mucosa. An aqueous solution of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers has been proposed by researchers to suppress oral bacterial adhesion to surfaces of teeth. In addition to increasing the possibility of infection due to bacterial adhesion, the presence of sutures on the oral wound can also increase postoperative complication, such as pain, swelling, and trismus. The aim of this thesis research was to evaluate whether a 5% aqueous solution of MPC can decrease retention and/or viability of oral bacteria from two different types of sutures that are commonly used in oral surgery: polypropylene and silk. This study also investigated whether the presence of a knot in the suture affects removal and/or viability of the oral bacteria from the sutures. This in vitro study was done by incubating the sutures in a mixture of TSB broth and freshly human pooled saliva for approximately 24 hours. The sutures then were treated by shaking for 90 seconds with 3 mouthrinses: chlorhexidine as a positive control, 5% MPC solution, or phosphate buffered saline (PBS) as a negative control. After treatments, the sutures were put into fresh TSB broth and incubated for 24 hours. The bacterial retention and/or viability was evaluated by measuring the turbidity of broth using a 1951 USAF Resolution Target. Contact angle analysis, scanning electron microscopy (SEM)/ energy dispersive x-ray spectroscopy (EDS), and infrared spectroscopy were used to characterize the suture materials and mouthrinse solutions. There was no significant difference in retention or viability of bacteria from the sutures after MPC mouthrinse treatment compared to the PBS control. However, chlorhexidine treatment resulted in significantly less bacterial viability compared to both MPC and PBS solutions. Whether chlorhexidine treatment also affected bacterial retention on the sutures cannot be determined from the protocol used in this study. Silk knot sutures presented the most bacteria retention/viability, whereas polypropylene straight sutures had the least in bacterial retention/viability. Chlorhexidine was the most effective antibacterial mouthrinse compared to 5% MPC and PBS mouthrinses. Type of suture materials and post-surgical mouthrinses should be considered while treating patients at high risk for infection.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Evaluation of Viable Bacterial Retention on Sutures After Three Different Mouthrinse Treatments"]}]}],"canonical_facts":{"dc:contributor":["Meyer, Anne","Biomaterials"],"dc:creator":["Gempita, Gema; 0000-0003-0621-5419"],"dc:date":["2025-02-21T21:44:43Z","2020"],"dc:description":["M.S.","In the oral cavity, sutures are continuously bathed in saliva containing approximately 7.5x108 microorganisms/ml which can attach to the suture surface and lead to prolonged infection. Chlorhexidine mouthrinse is considered to be the gold standard for antibacterial treatment in dentistry. However, it can have several negative side-effects in the mouth, such as discoloration on teeth surfaces and oral mucosa. An aqueous solution of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers has been proposed by researchers to suppress oral bacterial adhesion to surfaces of teeth. In addition to increasing the possibility of infection due to bacterial adhesion, the presence of sutures on the oral wound can also increase postoperative complication, such as pain, swelling, and trismus. The aim of this thesis research was to evaluate whether a 5% aqueous solution of MPC can decrease retention and/or viability of oral bacteria from two different types of sutures that are commonly used in oral surgery: polypropylene and silk. This study also investigated whether the presence of a knot in the suture affects removal and/or viability of the oral bacteria from the sutures. This in vitro study was done by incubating the sutures in a mixture of TSB broth and freshly human pooled saliva for approximately 24 hours. The sutures then were treated by shaking for 90 seconds with 3 mouthrinses: chlorhexidine as a positive control, 5% MPC solution, or phosphate buffered saline (PBS) as a negative control. After treatments, the sutures were put into fresh TSB broth and incubated for 24 hours. The bacterial retention and/or viability was evaluated by measuring the turbidity of broth using a 1951 USAF Resolution Target. Contact angle analysis, scanning electron microscopy (SEM)/ energy dispersive x-ray spectroscopy (EDS), and infrared spectroscopy were used to characterize the suture materials and mouthrinse solutions. There was no significant difference in retention or viability of bacteria from the sutures after MPC mouthrinse treatment compared to the PBS control. However, chlorhexidine treatment resulted in significantly less bacterial viability compared to both MPC and PBS solutions. Whether chlorhexidine treatment also affected bacterial retention on the sutures cannot be determined from the protocol used in this study. Silk knot sutures presented the most bacteria retention/viability, whereas polypropylene straight sutures had the least in bacterial retention/viability. Chlorhexidine was the most effective antibacterial mouthrinse compared to 5% MPC and PBS mouthrinses. Type of suture materials and post-surgical mouthrinses should be considered while treating patients at high risk for infection.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86751"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry"],"dc:title":["The Evaluation of Viable Bacterial Retention on Sutures After Three Different Mouthrinse Treatments"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:37Z"}