{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86688"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86688","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Investigation of Disease States Through Omics Based Techniques by Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Sekera, Emily; 0000-0002-1668-3227"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Wood, Troy","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T21:36:29Z","date_published":"2025-02-21T21:36:29Z","updated_at":"2026-07-27T19:05:34Z","subjects":["analytical chemistry","biochemistry","chemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86688","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wood, Troy","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Sekera, Emily; 0000-0002-1668-3227"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T21:36:29Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["analytical chemistry","biochemistry","chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86688"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","The study of –omics has rapidly gained momentum since its beginning with the advent of the Human Genome Project. As the human genome has been unraveled, studies of the downstream pathways have spurred from the resulting knowledge. Mutated genes have been found that change the directions for the creation of proteins, changing amino acid sequences, and in some instances causing its malfunction. With the protein no longer working properly, small molecules are produced in excess or inadequately for the body's needs. These changes can be seen throughout the body, from the tissue itself to biological fluids. The main objective of this thesis was to investigate biomarkers for multiple disease states using both -omics-based approaches in tandem with high resolution mass spectrometry. This work specifically focuses on (1) the validation of stercobilin as a putative biomarker for autism spectrum disorders across multiple murine models, (2) the sequencing of glucokinase, a protein hypothesized to play key roles in diabetes, to initiate the analysis of active binding sites, (3) the observation of lipid changes within an animal model of primary demyelination, mimicking both traumatic spinal cord injury and multiple sclerosis, to determine lipids which play a key role in the myelination process, and (4) the observation of neuropeptides within the brains of rats for their use in the study of drug addiction, specifically cocaine. The utilization of mass spectrometry to study biomolecules has become a golden standard owing to the introduction of two different ionization techniques, electrospray ionization (ESI) and matrix assisted desorption ionization (MALDI). The first two aims will discuss the utilization of ESI techniques for the study of small molecules, proteins, and peptides. Although the first aim will touch upon the use of MALDI for small molecule analysis, its utilization in the rapidly growing field of mass spectrometry imaging will be discussed within aims three and four to study lipids, and peptides.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Investigation of Disease States Through Omics Based Techniques by Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry"]}]}],"canonical_facts":{"dc:contributor":["Wood, Troy","Chemistry"],"dc:creator":["Sekera, Emily; 0000-0002-1668-3227"],"dc:date":["2025-02-21T21:36:29Z","2020"],"dc:description":["Ph.D.","The study of –omics has rapidly gained momentum since its beginning with the advent of the Human Genome Project. As the human genome has been unraveled, studies of the downstream pathways have spurred from the resulting knowledge. Mutated genes have been found that change the directions for the creation of proteins, changing amino acid sequences, and in some instances causing its malfunction. With the protein no longer working properly, small molecules are produced in excess or inadequately for the body's needs. These changes can be seen throughout the body, from the tissue itself to biological fluids. The main objective of this thesis was to investigate biomarkers for multiple disease states using both -omics-based approaches in tandem with high resolution mass spectrometry. This work specifically focuses on (1) the validation of stercobilin as a putative biomarker for autism spectrum disorders across multiple murine models, (2) the sequencing of glucokinase, a protein hypothesized to play key roles in diabetes, to initiate the analysis of active binding sites, (3) the observation of lipid changes within an animal model of primary demyelination, mimicking both traumatic spinal cord injury and multiple sclerosis, to determine lipids which play a key role in the myelination process, and (4) the observation of neuropeptides within the brains of rats for their use in the study of drug addiction, specifically cocaine. The utilization of mass spectrometry to study biomolecules has become a golden standard owing to the introduction of two different ionization techniques, electrospray ionization (ESI) and matrix assisted desorption ionization (MALDI). The first two aims will discuss the utilization of ESI techniques for the study of small molecules, proteins, and peptides. Although the first aim will touch upon the use of MALDI for small molecule analysis, its utilization in the rapidly growing field of mass spectrometry imaging will be discussed within aims three and four to study lipids, and peptides.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86688"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["analytical chemistry","biochemistry","chemistry"],"dc:title":["The Investigation of Disease States Through Omics Based Techniques by Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:34Z"}