{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86682"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86682","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Evaluation of ΔNp63 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinom","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Aljabri, Mohammed"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Romano, Rose-Anne","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T21:36:26Z","date_published":"2025-02-21T21:36:26Z","updated_at":"2026-07-27T19:05:34Z","subjects":["dentistry","pathology","biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86682","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Romano, Rose-Anne","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Aljabri, Mohammed"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T21:36:26Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry","pathology","biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86682"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Oral squamous cell carcinoma (OSCC) is by far the most common mucosal malignant neoplasm that arises in the head and neck, which accounts for more than 90% of all malignancies encountered in this anatomical region with the lateral border of the tongue being the most commonly affected area. Unfortunately, most patients with OSCC are diagnosed at a late stage of the disease. Despite the use of combined surgery and radiotherapy, still, there is 30% local or regional disease recurrence, 25% distal metastases, and a 40% five-year survival. Most often, progress to OSCC is preceded by oral epithelial dysplastic lesions such as leukoplakia and erythroplakia. Eventually, 16% to 36% of oral dysplasias transform into invasive OSCC. Thus, the need for early diagnosis and the development of robust biomarkers is of paramount importance. In recent years, studies from our lab and others have shed light upon the importance of the transcription factor p63 in the pathogenesis of oral dysplasia and SCC of different anatomical regions including the skin and the head and neck region. In fact, p63 overexpression or amplification is seen in more than 80% of OSCC. p63 is a member of the p53 tumor suppressor family and encodes for two major isoforms; TAp63 and ΔNp63, with ΔNp63 being the critical and predominant protein expressed in the epidermis and other epithelial tissues. Unlike other members of the p53 family, ΔNp63 acts as a proto-oncogene that when overexpressed leads to increase cell proliferation and inhibition of growth arrest signals. Overall, the oncogenic properties of ΔNp63 suggest that it may serve as a biomarker for OSCC progression. Given the importance of p63 in SCC, we sought to determine the pattern and levels of p63 expression in oral epithelial dysplasia and OSCCs cases using standard immunohistochemical staining in order to assess ΔNp63 usefulness as a potential biomarker for premalignant lesions. Fifty-one biopsy cases of human oral tissues were obtained from archives of The Department of Oral and Maxillofacial Pathology, University at Buffalo. The study consisted of six different categories including: mild epithelial dysplasia (n=10), moderate epithelial dysplasia (n=10), severe epithelial dysplasia (n=10), well-differentiated OSCC (n=10), moderately-differentiated OSCC (n=7) and poorly-differentiated OSCC (n=4) with a total of thirty cases of oral epithelial dysplasia (n=30) and twenty-one cases of OSCC (n=21). Immunohistochemical staining of ΔNp63 showed nuclear immunoreactivity in all cases with significantly increased ΔNp63 expression compared to the normal oral mucosa. The presence of strong ΔNp63 expression in areas beyond the parabasal cells suggests that these keratinocytes have an increased proliferative potential and aberrant differentiation, which in turn would predispose the cells to malignant transformation, and therefore may play an important role as a potential marker for premalignancy.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Evaluation of ΔNp63 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinom"]}]}],"canonical_facts":{"dc:contributor":["Romano, Rose-Anne","Oral Sciences"],"dc:creator":["Aljabri, Mohammed"],"dc:date":["2025-02-21T21:36:26Z","2020"],"dc:description":["M.S.","Oral squamous cell carcinoma (OSCC) is by far the most common mucosal malignant neoplasm that arises in the head and neck, which accounts for more than 90% of all malignancies encountered in this anatomical region with the lateral border of the tongue being the most commonly affected area. Unfortunately, most patients with OSCC are diagnosed at a late stage of the disease. Despite the use of combined surgery and radiotherapy, still, there is 30% local or regional disease recurrence, 25% distal metastases, and a 40% five-year survival. Most often, progress to OSCC is preceded by oral epithelial dysplastic lesions such as leukoplakia and erythroplakia. Eventually, 16% to 36% of oral dysplasias transform into invasive OSCC. Thus, the need for early diagnosis and the development of robust biomarkers is of paramount importance. In recent years, studies from our lab and others have shed light upon the importance of the transcription factor p63 in the pathogenesis of oral dysplasia and SCC of different anatomical regions including the skin and the head and neck region. In fact, p63 overexpression or amplification is seen in more than 80% of OSCC. p63 is a member of the p53 tumor suppressor family and encodes for two major isoforms; TAp63 and ΔNp63, with ΔNp63 being the critical and predominant protein expressed in the epidermis and other epithelial tissues. Unlike other members of the p53 family, ΔNp63 acts as a proto-oncogene that when overexpressed leads to increase cell proliferation and inhibition of growth arrest signals. Overall, the oncogenic properties of ΔNp63 suggest that it may serve as a biomarker for OSCC progression. Given the importance of p63 in SCC, we sought to determine the pattern and levels of p63 expression in oral epithelial dysplasia and OSCCs cases using standard immunohistochemical staining in order to assess ΔNp63 usefulness as a potential biomarker for premalignant lesions. Fifty-one biopsy cases of human oral tissues were obtained from archives of The Department of Oral and Maxillofacial Pathology, University at Buffalo. The study consisted of six different categories including: mild epithelial dysplasia (n=10), moderate epithelial dysplasia (n=10), severe epithelial dysplasia (n=10), well-differentiated OSCC (n=10), moderately-differentiated OSCC (n=7) and poorly-differentiated OSCC (n=4) with a total of thirty cases of oral epithelial dysplasia (n=30) and twenty-one cases of OSCC (n=21). Immunohistochemical staining of ΔNp63 showed nuclear immunoreactivity in all cases with significantly increased ΔNp63 expression compared to the normal oral mucosa. The presence of strong ΔNp63 expression in areas beyond the parabasal cells suggests that these keratinocytes have an increased proliferative potential and aberrant differentiation, which in turn would predispose the cells to malignant transformation, and therefore may play an important role as a potential marker for premalignancy.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86682"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry","pathology","biology"],"dc:title":["Evaluation of ΔNp63 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinom"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:34Z"}