{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86637"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86637","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Mechanisms of Manganese Trafficking Across the Brain Microvascular Endothelial Cell Barrier","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Steimle, Brittany; 0000-0003-3638-8767"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Kosman, Daniel","Biochemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T21:35:53Z","date_published":"2025-02-21T21:35:53Z","updated_at":"2026-07-27T19:05:32Z","subjects":["biochemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86637","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kosman, Daniel","Biochemistry"]},{"key":"dc:creator","label":"Author","values":["Steimle, Brittany; 0000-0003-3638-8767"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T21:35:53Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86637"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Manganese (Mn) is a fundamental co-factor required for numerous biochemical reactions. In the brain, this requirement is expanded to aid in glutamine synthetase reactions and in synaptic neurotransmission. Although essential, if not regulated properly, manganese can be toxic to cell biology and whole-body systems. This is particularly true in the case of brain Mn accumulation that is associated with neurologic diseases that resemble Parkinson's disease. This brain Mn toxicity can arise from chronic exposure of inhaling Mn-rich fumes in occupations like welding or ingesting Mn-contaminated drinking water. Additionally, manganese dysregulation may be inherited by influencing the proteins involved in normal Mn trafficking mechanisms. In this circumstance, hyperaccumulation of Mn in the brain stems from high Mn levels in the systemic circulation, which crosses into the brain interstitium. It is not clear whether the mismanagement of Mn is occurring at the endothelial barrier cells, or whether brain Mn entry is a secondary effect of high liver and blood Mn concentrations. In this thesis, the former possibility is examined. Circulating Mn enters into the brain via transcellular passage through brain microvascular endothelial cells (BMVEC), which make up the blood-brain barrier (BBB). The mechanisms of Mn trafficking in this context remain largely unexplored. The intention of this dissertation is to describe the current advancement of the overall understanding of Mn trafficking at the BBB via BMVEC. Specifically, I outline the proteins involved in Mn uptake and efflux in BMVEC, and furthermore the factors influencing the regulation of its distinguished flux at this barrier. These regulatory mechanisms highlight the importance of these transport pathways in maintaining a healthy brain Mn balance.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Mechanisms of Manganese Trafficking Across the Brain Microvascular Endothelial Cell Barrier"]}]}],"canonical_facts":{"dc:contributor":["Kosman, Daniel","Biochemistry"],"dc:creator":["Steimle, Brittany; 0000-0003-3638-8767"],"dc:date":["2025-02-21T21:35:53Z","2020"],"dc:description":["Ph.D.","Manganese (Mn) is a fundamental co-factor required for numerous biochemical reactions. In the brain, this requirement is expanded to aid in glutamine synthetase reactions and in synaptic neurotransmission. Although essential, if not regulated properly, manganese can be toxic to cell biology and whole-body systems. This is particularly true in the case of brain Mn accumulation that is associated with neurologic diseases that resemble Parkinson's disease. This brain Mn toxicity can arise from chronic exposure of inhaling Mn-rich fumes in occupations like welding or ingesting Mn-contaminated drinking water. Additionally, manganese dysregulation may be inherited by influencing the proteins involved in normal Mn trafficking mechanisms. In this circumstance, hyperaccumulation of Mn in the brain stems from high Mn levels in the systemic circulation, which crosses into the brain interstitium. It is not clear whether the mismanagement of Mn is occurring at the endothelial barrier cells, or whether brain Mn entry is a secondary effect of high liver and blood Mn concentrations. In this thesis, the former possibility is examined. Circulating Mn enters into the brain via transcellular passage through brain microvascular endothelial cells (BMVEC), which make up the blood-brain barrier (BBB). The mechanisms of Mn trafficking in this context remain largely unexplored. The intention of this dissertation is to describe the current advancement of the overall understanding of Mn trafficking at the BBB via BMVEC. Specifically, I outline the proteins involved in Mn uptake and efflux in BMVEC, and furthermore the factors influencing the regulation of its distinguished flux at this barrier. These regulatory mechanisms highlight the importance of these transport pathways in maintaining a healthy brain Mn balance.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86637"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["biochemistry"],"dc:title":["The Mechanisms of Manganese Trafficking Across the Brain Microvascular Endothelial Cell Barrier"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:32Z"}