{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86612"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86612","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Role of Candida albicans DFG5 and DCW1 in Regulation of Chitin Synthesis under Cell Wall and Morphogenetic Stress","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Sohi, Harleen"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Maddi, Abhiram","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T20:58:49Z","date_published":"2025-02-21T20:58:49Z","updated_at":"2026-07-27T19:05:32Z","subjects":["dentistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86612","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Maddi, Abhiram","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Sohi, Harleen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T20:58:49Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86612"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Objective: Dfg5 and Dcw1 are cell wall proteins in Candida albicans which play important roles in maintaining cell wall integrity. Past studies in our laboratory have shown that mutants of DFG5 and DCW1 are affected in basal Hog1 MAPK levels to regulate chitin synthesis in C. albicans. Parallel studies in our laboratory have indicated that Dfg5 and Dcw1 regulate chitin synthesis by transcriptional regulation of chitin synthases. The aim of this study is to compare the cell morphology of DFG5/DCW1 mutants and HOG1 mutants and to determine the changes in gene expression of chitin synthases under cell wall and morphogenetic stress conditions. Methods: Morphologic analysis of DFG5/DCW1 mutants and HOG1 mutants was performed under conditions of cell wall stress and hyphal induction. Scanning electron microscopy (SEM) analysis was performed to determine cell shape and morphology. Gene expression analysis of chitin synthase genes CHS1, CHS2, CHS3 and CHS8 was performed under cell wall stress and hyphal induction conditions. Results: The DFG5 and DCW1 mutants showed defective hyphal growth as compared to controls. There was change in cell shape and cell size in DFG5 and DCW1 mutants as compared to WT. The expression of chitin synthase genes CHS1, CHS2, CHS3 and CHS8 was increased in DFG5 and DCW1 mutants as well as HOG1 mutant in response to cell wall stress using calcofluor white (CFW). Calcium Chloride (CaCl2) was able to suppress and reverse this increased expression of chitin synthases that occurred in response to cell wall stress. Induction of hyphal growth completely suppressed the increased expression of chitin synthases. Conclusions: The data confirms that DFG5 and DCW1 are required for hyphae formation. Also, DFG5 and DCW1 mutants showed defects in cell polarity suggesting that DFG5 and DCW1 are important for maintaining cell shape. Several signaling pathways (PKC, HOG1 and Calcineurin) may function in coordination to control the expression of chitin synthase","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Role of Candida albicans DFG5 and DCW1 in Regulation of Chitin Synthesis under Cell Wall and Morphogenetic Stress"]}]}],"canonical_facts":{"dc:contributor":["Maddi, Abhiram","Oral Sciences"],"dc:creator":["Sohi, Harleen"],"dc:date":["2025-02-21T20:58:49Z","2020"],"dc:description":["M.S.","Objective: Dfg5 and Dcw1 are cell wall proteins in Candida albicans which play important roles in maintaining cell wall integrity. Past studies in our laboratory have shown that mutants of DFG5 and DCW1 are affected in basal Hog1 MAPK levels to regulate chitin synthesis in C. albicans. Parallel studies in our laboratory have indicated that Dfg5 and Dcw1 regulate chitin synthesis by transcriptional regulation of chitin synthases. The aim of this study is to compare the cell morphology of DFG5/DCW1 mutants and HOG1 mutants and to determine the changes in gene expression of chitin synthases under cell wall and morphogenetic stress conditions. Methods: Morphologic analysis of DFG5/DCW1 mutants and HOG1 mutants was performed under conditions of cell wall stress and hyphal induction. Scanning electron microscopy (SEM) analysis was performed to determine cell shape and morphology. Gene expression analysis of chitin synthase genes CHS1, CHS2, CHS3 and CHS8 was performed under cell wall stress and hyphal induction conditions. Results: The DFG5 and DCW1 mutants showed defective hyphal growth as compared to controls. There was change in cell shape and cell size in DFG5 and DCW1 mutants as compared to WT. The expression of chitin synthase genes CHS1, CHS2, CHS3 and CHS8 was increased in DFG5 and DCW1 mutants as well as HOG1 mutant in response to cell wall stress using calcofluor white (CFW). Calcium Chloride (CaCl2) was able to suppress and reverse this increased expression of chitin synthases that occurred in response to cell wall stress. Induction of hyphal growth completely suppressed the increased expression of chitin synthases. Conclusions: The data confirms that DFG5 and DCW1 are required for hyphae formation. Also, DFG5 and DCW1 mutants showed defects in cell polarity suggesting that DFG5 and DCW1 are important for maintaining cell shape. Several signaling pathways (PKC, HOG1 and Calcineurin) may function in coordination to control the expression of chitin synthase","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86612"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry"],"dc:title":["Role of Candida albicans DFG5 and DCW1 in Regulation of Chitin Synthesis under Cell Wall and Morphogenetic Stress"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:32Z"}