{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/86489"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/86489","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Effects of Central α7 Nicotinic Acetylcholine Receptors on Energy Intake and Body Weight in Rats","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["DiBrog, Adrianne; 0000-0002-6738-6628"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Mietlicki-Baase, Elizabeth","Exercise and Nutrition Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-02-21T17:22:56Z","date_published":"2025-02-21T17:22:56Z","updated_at":"2026-07-27T19:05:32Z","subjects":["nutrition"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/86489","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mietlicki-Baase, Elizabeth","Exercise and Nutrition Sciences"]},{"key":"dc:creator","label":"Author","values":["DiBrog, Adrianne; 0000-0002-6738-6628"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-02-21T17:22:56Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/86489"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Obesity is a prevalent disease, but effective treatments remain limited. Agonists of the alpha-7 nicotinic acetylcholine receptor (α7nAChR) promote negative energy balance in mice, but these effects are not well-studied in rats. The α7nAChR agonist GTS-21 has been shown to increase the food intake-suppressive incretin hormone glucagon-like peptide-1 (GLP-1) in mice, suggesting that GTS-21 may produce hypophagia via a GLP-1-related mechanism. We tested the hypothesis that GTS-21 would decrease food intake and body weight in adult male Sprague Dawley rats. In a dose-response study of feeding and body weight change after acute intracerebroventricular (ICV) injection of GTS-21, contrary to our hypothesis, GTS-21 had no impact (p>0.05) on food intake and increased (p<0.05) body weight at the highest dose tested (0.5mg). As endogenous GLP-1 is rapidly degraded by dipeptidyl peptidase-IV (DPP-IV), we hypothesized that if GTS-21 induces GLP-1 release in rats, DPP-IV-mediated degradation of GLP-1 may mask feeding effects of GTS-21. We combined ICV administration of the DPP-IV inhibitor sitagliptin (SIT) with ICV GTS-21 and measured feeding effects when rats had a choice of both chow and palatable high-fat diet (HFD). Rats ate more chow with SIT, GTS-21, or SIT+GTS-21 at 1h post-injection (p<0.05), with no effect on HFD intake. Main effects of SIT and GTS-21 to increase chow intake were found at 3h. At 3h, SIT-treated rats also had lower preference for HFD than vehicle-treated rats. Finally, we hypothesized that a more selective agonist of the α7nAChR may produce stronger effects. ICV administration of the selective α7nAChR agonist PHA-543613 produced a numerical, but non-significant, decrease in body weight and intake of HFD, with no effect on chow intake. Collectively, our studies suggest a more selective agonist of the α7nAChR may be needed to produce a negative energy balance effect. Furthermore, lateral ventricle injection of SIT may induce a shift in food preference, favoring lower calorie food options.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Effects of Central α7 Nicotinic Acetylcholine Receptors on Energy Intake and Body Weight in Rats"]}]}],"canonical_facts":{"dc:contributor":["Mietlicki-Baase, Elizabeth","Exercise and Nutrition Sciences"],"dc:creator":["DiBrog, Adrianne; 0000-0002-6738-6628"],"dc:date":["2025-02-21T17:22:56Z","2020"],"dc:description":["M.S.","Obesity is a prevalent disease, but effective treatments remain limited. Agonists of the alpha-7 nicotinic acetylcholine receptor (α7nAChR) promote negative energy balance in mice, but these effects are not well-studied in rats. The α7nAChR agonist GTS-21 has been shown to increase the food intake-suppressive incretin hormone glucagon-like peptide-1 (GLP-1) in mice, suggesting that GTS-21 may produce hypophagia via a GLP-1-related mechanism. We tested the hypothesis that GTS-21 would decrease food intake and body weight in adult male Sprague Dawley rats. In a dose-response study of feeding and body weight change after acute intracerebroventricular (ICV) injection of GTS-21, contrary to our hypothesis, GTS-21 had no impact (p>0.05) on food intake and increased (p<0.05) body weight at the highest dose tested (0.5mg). As endogenous GLP-1 is rapidly degraded by dipeptidyl peptidase-IV (DPP-IV), we hypothesized that if GTS-21 induces GLP-1 release in rats, DPP-IV-mediated degradation of GLP-1 may mask feeding effects of GTS-21. We combined ICV administration of the DPP-IV inhibitor sitagliptin (SIT) with ICV GTS-21 and measured feeding effects when rats had a choice of both chow and palatable high-fat diet (HFD). Rats ate more chow with SIT, GTS-21, or SIT+GTS-21 at 1h post-injection (p<0.05), with no effect on HFD intake. Main effects of SIT and GTS-21 to increase chow intake were found at 3h. At 3h, SIT-treated rats also had lower preference for HFD than vehicle-treated rats. Finally, we hypothesized that a more selective agonist of the α7nAChR may produce stronger effects. ICV administration of the selective α7nAChR agonist PHA-543613 produced a numerical, but non-significant, decrease in body weight and intake of HFD, with no effect on chow intake. Collectively, our studies suggest a more selective agonist of the α7nAChR may be needed to produce a negative energy balance effect. Furthermore, lateral ventricle injection of SIT may induce a shift in food preference, favoring lower calorie food options.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/86489"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["nutrition"],"dc:title":["Effects of Central α7 Nicotinic Acetylcholine Receptors on Energy Intake and Body Weight in Rats"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:32Z"}