{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/84097"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/84097","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Design and Validation of Passive Environmental DNA Sampler (PEDS)","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Kirtane, Anish; 0000-0001-8898-4275"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Sassoubre, Lauren","Civil, Structural and Environmental Engineering"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-06-21T15:47:54Z","date_published":"2022-06-21T15:47:54Z","updated_at":"2026-07-27T19:05:30Z","subjects":["environmental engineering","molecular biology","environmental science"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/84097","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sassoubre, Lauren","Civil, Structural and Environmental Engineering"]},{"key":"dc:creator","label":"Author","values":["Kirtane, Anish; 0000-0001-8898-4275"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-06-21T15:47:54Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["environmental engineering","molecular biology","environmental science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/84097"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Environmental DNA (eDNA) shed by organisms into their environments has gained immense prominence as a tool for species and biodiversity monitoring. In aquatic environments, eDNA is captured in a grab sample that is filtered to concentrate the sample before eDNA extraction and analysis. Current capture and filtration methods have several limitations: 1) inability to capture all extracellular DNA, 2) eDNA degradation during sample transport and storage, 3) filter clogging in turbid waters which influences detection of rare organisms, and 4) time sensitivity due to pulse inputs of eDNA. To address these limitations, we designed and validated the Passive Environmental DNA Sampler (PEDS). A PEDS consists of an absorbent-filled sachet that can be suspended in water to collect eDNA over long timescales. eDNA captured by a PEDS is protected from degradation and represents a time weighted average instead of a grab sample snapshot. Two adsorbents, Montmorillonite Clay (MC) and Granular Activated Carbon (GAC), were tested in laboratory, microcosm and field experiments. Laboratory results indicated that MC better adsorbed and desorbed extracellular DNA. However, GAC PEDS more effectively captured eDNA in microcosm and field experiments. Field results further validated PEDS as an effective eDNA capture method comparable to traditional water filtration of a grab samples.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Design and Validation of Passive Environmental DNA Sampler (PEDS)"]}]}],"canonical_facts":{"dc:contributor":["Sassoubre, Lauren","Civil, Structural and Environmental Engineering"],"dc:creator":["Kirtane, Anish; 0000-0001-8898-4275"],"dc:date":["2022-06-21T15:47:54Z","2020"],"dc:description":["M.S.","Environmental DNA (eDNA) shed by organisms into their environments has gained immense prominence as a tool for species and biodiversity monitoring. In aquatic environments, eDNA is captured in a grab sample that is filtered to concentrate the sample before eDNA extraction and analysis. Current capture and filtration methods have several limitations: 1) inability to capture all extracellular DNA, 2) eDNA degradation during sample transport and storage, 3) filter clogging in turbid waters which influences detection of rare organisms, and 4) time sensitivity due to pulse inputs of eDNA. To address these limitations, we designed and validated the Passive Environmental DNA Sampler (PEDS). A PEDS consists of an absorbent-filled sachet that can be suspended in water to collect eDNA over long timescales. eDNA captured by a PEDS is protected from degradation and represents a time weighted average instead of a grab sample snapshot. Two adsorbents, Montmorillonite Clay (MC) and Granular Activated Carbon (GAC), were tested in laboratory, microcosm and field experiments. Laboratory results indicated that MC better adsorbed and desorbed extracellular DNA. However, GAC PEDS more effectively captured eDNA in microcosm and field experiments. Field results further validated PEDS as an effective eDNA capture method comparable to traditional water filtration of a grab samples.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/84097"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["environmental engineering","molecular biology","environmental science"],"dc:title":["Design and Validation of Passive Environmental DNA Sampler (PEDS)"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:30Z"}