{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/84054"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/84054","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Discovery and Investigation of Functional Roles for Very Long Chain Fatty Acids during Necroptosis","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Parisi, Laura; 0000-0003-4868-1426"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Atilla-Gokcumen, G. Ekin","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-06-21T15:47:27Z","date_published":"2022-06-21T15:47:27Z","updated_at":"2026-07-27T19:05:30Z","subjects":["biochemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/84054","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Atilla-Gokcumen, G. Ekin","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Parisi, Laura; 0000-0003-4868-1426"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-06-21T15:47:27Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/84054"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Lipids are essential biomolecules that have a fundamental role in maintaining membrane architecture. They are also important molecules for energy storage and can have specialized roles as signaling molecules and second messengers. Our cells produce thousands of different lipid species, and there is a growing appreciation that subtle differences in chemical structure, even within a given class of lipids, can have a profound effect on biological function, and that lipids can be mediators of critical cellular processes such as cell division and cell death. In this dissertation, I will discuss efforts toward identifying novel functional roles of lipids during necroptosis, a form of regulated necrotic cell death. We utilized an untargeted liquid chromatography-mass spectrometry (LC-MS)-based approach in order to explore the lipidomes of control vs. necroptotic cells. This approach allowed us to identify lipids whose levels changed significantly during necroptosis. We hypothesized that these changes may play a functional role in necroptotic membrane permeabilization and cell death. We found that there was a general accumulation of ceramides during necroptosis, which has also been observed by other researchers who investigated changes in sphingolipids during necroptosis using a targeted LC-MS-based approach. More interestingly, we also discovered an accumulation of saturated very long chain fatty acids (VLCFAs), which contain ≥ 20 carbons in their acyl chain. These lipids have not been previously connected to regulated cell death. However, we envisioned that these lipids could have profound effects on the structure of cellular membranes, due to their mismatch in chain length with membrane lipids that contain C16 and C18 fatty acyl chains.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Discovery and Investigation of Functional Roles for Very Long Chain Fatty Acids during Necroptosis"]}]}],"canonical_facts":{"dc:contributor":["Atilla-Gokcumen, G. Ekin","Chemistry"],"dc:creator":["Parisi, Laura; 0000-0003-4868-1426"],"dc:date":["2022-06-21T15:47:27Z","2020"],"dc:description":["Ph.D.","Lipids are essential biomolecules that have a fundamental role in maintaining membrane architecture. They are also important molecules for energy storage and can have specialized roles as signaling molecules and second messengers. Our cells produce thousands of different lipid species, and there is a growing appreciation that subtle differences in chemical structure, even within a given class of lipids, can have a profound effect on biological function, and that lipids can be mediators of critical cellular processes such as cell division and cell death. In this dissertation, I will discuss efforts toward identifying novel functional roles of lipids during necroptosis, a form of regulated necrotic cell death. We utilized an untargeted liquid chromatography-mass spectrometry (LC-MS)-based approach in order to explore the lipidomes of control vs. necroptotic cells. This approach allowed us to identify lipids whose levels changed significantly during necroptosis. We hypothesized that these changes may play a functional role in necroptotic membrane permeabilization and cell death. We found that there was a general accumulation of ceramides during necroptosis, which has also been observed by other researchers who investigated changes in sphingolipids during necroptosis using a targeted LC-MS-based approach. More interestingly, we also discovered an accumulation of saturated very long chain fatty acids (VLCFAs), which contain ≥ 20 carbons in their acyl chain. These lipids have not been previously connected to regulated cell death. However, we envisioned that these lipids could have profound effects on the structure of cellular membranes, due to their mismatch in chain length with membrane lipids that contain C16 and C18 fatty acyl chains.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/84054"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["biochemistry"],"dc:title":["Discovery and Investigation of Functional Roles for Very Long Chain Fatty Acids during Necroptosis"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:30Z"}