{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/84047"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/84047","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Testing Physical Properties and In vitro Cytocompatibility of Chitosan-Based Hydrogel and Hydrogel with Mustard Powder for Joint Lubrication","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Vemuri, Madhurima; 0000-0002-6655-8272"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Meyer, Anne","Biomaterials"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-06-21T15:47:23Z","date_published":"2022-06-21T15:47:23Z","updated_at":"2026-07-27T19:05:30Z","subjects":["materials science","polymer chemistry","cellular biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/84047","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Meyer, Anne","Biomaterials"]},{"key":"dc:creator","label":"Author","values":["Vemuri, Madhurima; 0000-0002-6655-8272"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-06-21T15:47:23Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["materials science","polymer chemistry","cellular biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/84047"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Osteoarthritis (OA) is one of the leading factors influencing the quality of life, most common in older age groups but not uncommon in younger groups. OA can affect any joint in the body. Mainly affected joints are “stress bearing” (e.g. knee, hip, hands, feet). It can also affect “non stress bearing joints” (e.g. shoulder, wrist, ankle). Various treatments of OA currently include pharmacological methods (NSAIDS, GABA receptor blockers, intra-articular injections) and non-pharmacological methods (surgical intervention, acupuncture and rehabilitation).This thesis research was primarily based on a concept of intra-articular injection of a chitosan/glycerol-phosphate (C/Gp) hydrogel in comparison with two formulations containing mustard powder (M) as a potential antioxidant. C/Gp/M hydrogels were prepared by subjecting the chitosan solution and glycerol phosphate solutions to -17oC separately, before mixing to form the hydrogel. Cytotoxicity on Human Calvarial Osteoblasts (HCO) cells (in-vitro) was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay after a 48-hour culture period. Gel deformation by compression at room temperature and coefficient of friction were evaluated. Based on the MTT data, results showed that C/Gp and C/Gp/M (0.002gm/10ml of gel) produced greater mitochondrial activity when compared to an agar control (p=0.001, p=0.001 respectively). There was no statistically significant difference between agar and C/Gp/M (0.02gm/10ml) (p=0.82). After medium was conditioned with hydrogels (with and without mustard) for 48 hours, there was no statistically significant difference between the no-gel control and conditioned medium (p=0.23). It was observed that as cell culture well surface area was increased, the mitochondrial activity in the group with gels was similar to the no-gel control. Compression tests indicated that the hydrogels with pre-exposure temperature of -17C can take up more load before deformation (p=0.0001). Tissue-on-tissue coefficient of friction indicated the hydrogels can reduce friction from approximately 0.45 to approximately 0.15 (n=1 per condition).Based on the MTT data, results showed that C/Gp and two formulations of C/Gp/M (0.02gm/10ml and 0.002gm/10ml) were not cytotoxic. Microscopic spreading of gel in culture wells decreased mitochondrial activity in smaller wells, relative to no-gel control wells. Gels can reduce friction, supporting use as a joint lubricant. Further analysis is required for the formulated hydrogel including increasing sample size for different analyses, testing if material could reduce inflammatory markers (reactive oxygen species) in a cell culture or animal model, confirming mustard particle dissolution and equal distribution in hydrogel, and determining extent of release of particles from the hydrogel.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Testing Physical Properties and In vitro Cytocompatibility of Chitosan-Based Hydrogel and Hydrogel with Mustard Powder for Joint Lubrication"]}]}],"canonical_facts":{"dc:contributor":["Meyer, Anne","Biomaterials"],"dc:creator":["Vemuri, Madhurima; 0000-0002-6655-8272"],"dc:date":["2022-06-21T15:47:23Z","2020"],"dc:description":["M.S.","Osteoarthritis (OA) is one of the leading factors influencing the quality of life, most common in older age groups but not uncommon in younger groups. OA can affect any joint in the body. Mainly affected joints are “stress bearing” (e.g. knee, hip, hands, feet). It can also affect “non stress bearing joints” (e.g. shoulder, wrist, ankle). Various treatments of OA currently include pharmacological methods (NSAIDS, GABA receptor blockers, intra-articular injections) and non-pharmacological methods (surgical intervention, acupuncture and rehabilitation).This thesis research was primarily based on a concept of intra-articular injection of a chitosan/glycerol-phosphate (C/Gp) hydrogel in comparison with two formulations containing mustard powder (M) as a potential antioxidant. C/Gp/M hydrogels were prepared by subjecting the chitosan solution and glycerol phosphate solutions to -17oC separately, before mixing to form the hydrogel. Cytotoxicity on Human Calvarial Osteoblasts (HCO) cells (in-vitro) was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay after a 48-hour culture period. Gel deformation by compression at room temperature and coefficient of friction were evaluated. Based on the MTT data, results showed that C/Gp and C/Gp/M (0.002gm/10ml of gel) produced greater mitochondrial activity when compared to an agar control (p=0.001, p=0.001 respectively). There was no statistically significant difference between agar and C/Gp/M (0.02gm/10ml) (p=0.82). After medium was conditioned with hydrogels (with and without mustard) for 48 hours, there was no statistically significant difference between the no-gel control and conditioned medium (p=0.23). It was observed that as cell culture well surface area was increased, the mitochondrial activity in the group with gels was similar to the no-gel control. Compression tests indicated that the hydrogels with pre-exposure temperature of -17C can take up more load before deformation (p=0.0001). Tissue-on-tissue coefficient of friction indicated the hydrogels can reduce friction from approximately 0.45 to approximately 0.15 (n=1 per condition).Based on the MTT data, results showed that C/Gp and two formulations of C/Gp/M (0.02gm/10ml and 0.002gm/10ml) were not cytotoxic. Microscopic spreading of gel in culture wells decreased mitochondrial activity in smaller wells, relative to no-gel control wells. Gels can reduce friction, supporting use as a joint lubricant. Further analysis is required for the formulated hydrogel including increasing sample size for different analyses, testing if material could reduce inflammatory markers (reactive oxygen species) in a cell culture or animal model, confirming mustard particle dissolution and equal distribution in hydrogel, and determining extent of release of particles from the hydrogel.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/84047"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["materials science","polymer chemistry","cellular biology"],"dc:title":["Testing Physical Properties and In vitro Cytocompatibility of Chitosan-Based Hydrogel and Hydrogel with Mustard Powder for Joint Lubrication"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:30Z"}