{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/84027"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/84027","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Defining the Functions of TRPM4 and PLCβ3 in Taste Cell Signaling","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Dutta Banik, Debarghya"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Medler, Kathryn","Biological Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-06-21T15:47:11Z","date_published":"2022-06-21T15:47:11Z","updated_at":"2026-07-27T19:05:30Z","subjects":["neurosciences"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/84027","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Medler, Kathryn","Biological Sciences"]},{"key":"dc:creator","label":"Author","values":["Dutta Banik, Debarghya"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-06-21T15:47:11Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["neurosciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/84027"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","The taste system evolved as a way to detect nutrients and toxins in the external environment. This system is critical for survival because without the taste system, an organism could not detect beneficial compounds or avoid harmful compounds. To detect environmental chemicals, taste receptor cells use a variety of signaling pathways. Some of these signaling pathways are well-established, whereas some are not. The goal of my dissertation is to identify new proteins and pathways that are involved in the transduction of taste stimuli. My first study identified a previously unknown role for TRPM4 in the transduction of bitter, sweet, and umami stimuli. TRPM4 belongs to the transient receptor potential (TRP) family of non-selective cation channels and regulates membrane potential in other systems. Loss of TRPM4 in taste cells severely impairs both cellular and behavioral responses to these stimuli (Chapter II). In my second study, I characterized a new taste cell population that uses a PLCβ3/IP3R1 signaling pathway to detect bitter, sweet, and umami stimuli. These cells respond to multiple taste stimuli across taste modalities, and are called broadly responsive (BR) cells. My data found that loss of BR taste cells abolished central taste activity and taste-driven behaviors, demonstrating that these cells are important for taste transduction (Chapter III). I also found that TRPM4 works downstream of the PLCβ3 signaling pathway in BR taste cells. In these cells, TRPM4 modulates the membrane potential to regulate the L-type VGCC activity and links the PLCβ3 signaling pathway with the events that cause neurotransmitter release (Chapter IV). Taken together my studies demonstrate that taste signaling is much complex than previously thought.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Defining the Functions of TRPM4 and PLCβ3 in Taste Cell Signaling"]}]}],"canonical_facts":{"dc:contributor":["Medler, Kathryn","Biological Sciences"],"dc:creator":["Dutta Banik, Debarghya"],"dc:date":["2022-06-21T15:47:11Z","2020"],"dc:description":["Ph.D.","The taste system evolved as a way to detect nutrients and toxins in the external environment. This system is critical for survival because without the taste system, an organism could not detect beneficial compounds or avoid harmful compounds. To detect environmental chemicals, taste receptor cells use a variety of signaling pathways. Some of these signaling pathways are well-established, whereas some are not. The goal of my dissertation is to identify new proteins and pathways that are involved in the transduction of taste stimuli. My first study identified a previously unknown role for TRPM4 in the transduction of bitter, sweet, and umami stimuli. TRPM4 belongs to the transient receptor potential (TRP) family of non-selective cation channels and regulates membrane potential in other systems. Loss of TRPM4 in taste cells severely impairs both cellular and behavioral responses to these stimuli (Chapter II). In my second study, I characterized a new taste cell population that uses a PLCβ3/IP3R1 signaling pathway to detect bitter, sweet, and umami stimuli. These cells respond to multiple taste stimuli across taste modalities, and are called broadly responsive (BR) cells. My data found that loss of BR taste cells abolished central taste activity and taste-driven behaviors, demonstrating that these cells are important for taste transduction (Chapter III). I also found that TRPM4 works downstream of the PLCβ3 signaling pathway in BR taste cells. In these cells, TRPM4 modulates the membrane potential to regulate the L-type VGCC activity and links the PLCβ3 signaling pathway with the events that cause neurotransmitter release (Chapter IV). Taken together my studies demonstrate that taste signaling is much complex than previously thought.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/84027"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["neurosciences"],"dc:title":["Defining the Functions of TRPM4 and PLCβ3 in Taste Cell Signaling"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:30Z"}