{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/84018"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/84018","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Use of Graphene as a Substrate for Traditional Matrices in Matrix-Assisted Laser Desorption Ionization Mass Spectrometry","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Mascaro, Kayla"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Wood, Troy","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-06-21T15:47:05Z","date_published":"2022-06-21T15:47:05Z","updated_at":"2026-07-27T19:05:28Z","subjects":["chemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/84018","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wood, Troy","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Mascaro, Kayla"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-06-21T15:47:05Z","2020"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/84018"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is a useful technique for many types of analysis including pathology and disease biomarkers, but a major limitation is the spatial resolution. In recent years, graphene, a single layer of carbon atoms, has emerged as a possible MALDI matrix capable of combating this limitation. Previous work in our group has shown that graphene has low background noise from salt adducts and matrix clusters found in typical MALDI matrices. In this study, graphene is used in imaging studies as a matrix in conjunction with traditional MALDI matrices to improve homogeneity across samples. The samples are analyzed using Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry. The co-crystallization of the matrix and analyte was significantly improved in the samples deposited on graphene. Atomic force microscopy (AFC) and Raman were used to characterize the graphene used in the following experiments. Shot-to-shot variability and spot-to-spot variability were tested to confirm that the use of graphene increases homogeneity. Calibration curves were also created as an attempt to use MALDI with graphene as a quantitative method. In the future, we plan to explore the use of multiple layers of graphene as a matrix as well as alternative sources of graphene. We also plan on continuing work using CHCA sublimation and using MALDI quantitatively. After performing those studies, we aim to apply the information learned to use graphene with traditional MALDI matrices on tissue samples.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Use of Graphene as a Substrate for Traditional Matrices in Matrix-Assisted Laser Desorption Ionization Mass Spectrometry"]}]}],"canonical_facts":{"dc:contributor":["Wood, Troy","Chemistry"],"dc:creator":["Mascaro, Kayla"],"dc:date":["2022-06-21T15:47:05Z","2020"],"dc:description":["M.S.","Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is a useful technique for many types of analysis including pathology and disease biomarkers, but a major limitation is the spatial resolution. In recent years, graphene, a single layer of carbon atoms, has emerged as a possible MALDI matrix capable of combating this limitation. Previous work in our group has shown that graphene has low background noise from salt adducts and matrix clusters found in typical MALDI matrices. In this study, graphene is used in imaging studies as a matrix in conjunction with traditional MALDI matrices to improve homogeneity across samples. The samples are analyzed using Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry. The co-crystallization of the matrix and analyte was significantly improved in the samples deposited on graphene. Atomic force microscopy (AFC) and Raman were used to characterize the graphene used in the following experiments. Shot-to-shot variability and spot-to-spot variability were tested to confirm that the use of graphene increases homogeneity. Calibration curves were also created as an attempt to use MALDI with graphene as a quantitative method. In the future, we plan to explore the use of multiple layers of graphene as a matrix as well as alternative sources of graphene. We also plan on continuing work using CHCA sublimation and using MALDI quantitatively. After performing those studies, we aim to apply the information learned to use graphene with traditional MALDI matrices on tissue samples.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/84018"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["chemistry"],"dc:title":["The Use of Graphene as a Substrate for Traditional Matrices in Matrix-Assisted Laser Desorption Ionization Mass Spectrometry"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:28Z"}