{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/83067"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/83067","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Naive Pluripotency in Human Attained by Transient Inhibition of mTOR","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Li, Hanqin"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Feng, Jian","Neuroscience"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-08-16T18:04:32Z","date_published":"2021-08-16T18:04:32Z","updated_at":"2026-07-27T19:05:28Z","subjects":["neurosciences","mTOR","naive pluripotency","pluripotent","TFE3"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/83067","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Feng, Jian","Neuroscience"]},{"key":"dc:creator","label":"Author","values":["Li, Hanqin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-08-16T18:04:32Z","2019"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["neurosciences","mTOR","naive pluripotency","pluripotent","TFE3"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/83067"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","It has not been possible to generate naive human pluripotent stem cells (hPSCs) that significantly contribute to mouse embryos. We found that a brief inhibition of mTOR with Torin1 converted hPSCs from primed to naive pluripotency. The naive hPSCs were maintained in the same condition as mouse embryonic stem cells and exhibited high clonogenicity, rapid proliferation, mitochondrial respiration, X chromosome reactivation, DNA hypomethylation, and transcriptomes similar to those of human blastocysts. When transferred to mouse blastocysts, naive hPSCs robustly contributed to human cells of all three germ layers, including large amounts of enucleated red blood cells and photoreceptors, suggesting a significant acceleration of hPSC development in mouse embryos. Torin1 induced nuclear translocation of TFE3; TFE3 with mutated nuclear localization signal blocked the primed-to-naive conversion. The generation of chimera-competent naive hPSCs unified the ground state of pluripotency in mammals and may enable applications such as human organ generation in chimeric animals.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Naive Pluripotency in Human Attained by Transient Inhibition of mTOR"]}]}],"canonical_facts":{"dc:contributor":["Feng, Jian","Neuroscience"],"dc:creator":["Li, Hanqin"],"dc:date":["2021-08-16T18:04:32Z","2019"],"dc:description":["Ph.D.","It has not been possible to generate naive human pluripotent stem cells (hPSCs) that significantly contribute to mouse embryos. We found that a brief inhibition of mTOR with Torin1 converted hPSCs from primed to naive pluripotency. The naive hPSCs were maintained in the same condition as mouse embryonic stem cells and exhibited high clonogenicity, rapid proliferation, mitochondrial respiration, X chromosome reactivation, DNA hypomethylation, and transcriptomes similar to those of human blastocysts. When transferred to mouse blastocysts, naive hPSCs robustly contributed to human cells of all three germ layers, including large amounts of enucleated red blood cells and photoreceptors, suggesting a significant acceleration of hPSC development in mouse embryos. Torin1 induced nuclear translocation of TFE3; TFE3 with mutated nuclear localization signal blocked the primed-to-naive conversion. The generation of chimera-competent naive hPSCs unified the ground state of pluripotency in mammals and may enable applications such as human organ generation in chimeric animals.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/83067"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["neurosciences","mTOR","naive pluripotency","pluripotent","TFE3"],"dc:title":["Naive Pluripotency in Human Attained by Transient Inhibition of mTOR"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:28Z"}