{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80939"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80939","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Comparison of Dental Sealants for Antimicrobial Effects on Three Strains of Streptococcus mutans","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Mascaro, Madelyn; 0000-0002-3434-6558"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Meyer, Anne","Biomaterials"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-29T16:48:19Z","date_published":"2019-10-29T16:48:19Z","updated_at":"2026-07-27T19:05:28Z","subjects":["materials science"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80939","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Meyer, Anne","Biomaterials"]},{"key":"dc:creator","label":"Author","values":["Mascaro, Madelyn; 0000-0002-3434-6558"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-29T16:48:19Z","2019","2019-08-09 09:55:01"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["materials science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80939"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Early childhood caries is the most prevalent disease in children. Silver diamine fluoride (SDF) is an inexpensive and noninvasive topical agent that can be used as a dental sealant, which is a commonly used material to prevent dental caries. However, little is known about its antimicrobial properties on non-carious hydroxyapatite. The aim of the thesis research was to compare the in-vitro antimicrobial properties between SDF, alone, and SDF with an immediate application of potassium iodide (KI) on three strains of Streptococcus mutans (ATCC 10449, NG8, OMZ175), a predominant cause of dental caries. Potassium iodide is applied after the SDF to lessen the black staining to the tooth surface. All treatments were applied to calcium hydroxyapatite disks. Control treatments included a glass ionomer sealant and a chlorhexidine gluconate antiseptic solution. Contact angle analysis, scanning electron microscopy (SEM)/ energy dispersive x-ray spectroscopy (EDS), and infrared spectroscopy were used to characterize the treatment coatings on the hydroxyapatite disks. An agar diffusion assay and statistical analyses were utilized to determine which material had better antimicrobial properties within 24 and 48 h of anaerobic incubation at 37°C in a candle jar. SDF alone proved to be a significantly better antimicrobial (p<0.05) than SDF/KI against the ATCC 10449 strain. A difference in concentration and distribution of silver ions across the hydroxyapatite disk was observed by SEM/EDS. Chlorhexidine gluconate, the positive control, showed the best antimicrobial properties whereas the glass ionomer sealant did not show any. Based on these data, SDF alone appears to have better antimicrobial properties. Future work includes comparing the ability of these dental sealants to inhibit biofilm formation, and to test their antimicrobial properties against commensal bacteria."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparison of Dental Sealants for Antimicrobial Effects on Three Strains of Streptococcus mutans"]}]}],"canonical_facts":{"dc:contributor":["Meyer, Anne","Biomaterials"],"dc:creator":["Mascaro, Madelyn; 0000-0002-3434-6558"],"dc:date":["2019-10-29T16:48:19Z","2019","2019-08-09 09:55:01"],"dc:description":["M.S.","Early childhood caries is the most prevalent disease in children. Silver diamine fluoride (SDF) is an inexpensive and noninvasive topical agent that can be used as a dental sealant, which is a commonly used material to prevent dental caries. However, little is known about its antimicrobial properties on non-carious hydroxyapatite. The aim of the thesis research was to compare the in-vitro antimicrobial properties between SDF, alone, and SDF with an immediate application of potassium iodide (KI) on three strains of Streptococcus mutans (ATCC 10449, NG8, OMZ175), a predominant cause of dental caries. Potassium iodide is applied after the SDF to lessen the black staining to the tooth surface. All treatments were applied to calcium hydroxyapatite disks. Control treatments included a glass ionomer sealant and a chlorhexidine gluconate antiseptic solution. Contact angle analysis, scanning electron microscopy (SEM)/ energy dispersive x-ray spectroscopy (EDS), and infrared spectroscopy were used to characterize the treatment coatings on the hydroxyapatite disks. An agar diffusion assay and statistical analyses were utilized to determine which material had better antimicrobial properties within 24 and 48 h of anaerobic incubation at 37°C in a candle jar. SDF alone proved to be a significantly better antimicrobial (p<0.05) than SDF/KI against the ATCC 10449 strain. A difference in concentration and distribution of silver ions across the hydroxyapatite disk was observed by SEM/EDS. Chlorhexidine gluconate, the positive control, showed the best antimicrobial properties whereas the glass ionomer sealant did not show any. Based on these data, SDF alone appears to have better antimicrobial properties. Future work includes comparing the ability of these dental sealants to inhibit biofilm formation, and to test their antimicrobial properties against commensal bacteria."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80939"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["materials science"],"dc:title":["Comparison of Dental Sealants for Antimicrobial Effects on Three Strains of Streptococcus mutans"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:28Z"}