{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80928"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80928","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Analysis of Compartmental Dynamics of Heat Shock Response in Caenorhabditis elegans","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Annis, Jillian"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gunawan, Rudiyanto","Chemical and Biological Engineering"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-29T16:48:14Z","date_published":"2019-10-29T16:48:14Z","updated_at":"2026-07-27T19:05:28Z","subjects":["molecular biology","aging"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80928","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gunawan, Rudiyanto","Chemical and Biological Engineering"]},{"key":"dc:creator","label":"Author","values":["Annis, Jillian"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-29T16:48:14Z","2019","2019-08-08 15:56:51"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["molecular biology","aging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80928"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Caenorhabditis elegans is one of the most widely used model organisms in biological research. Its innate characteristics such as small size, transparency, and high degree of genetic homology with humans in conjunction with the large community dedicated to its study help make it an important model for studying highly conserved biological processes. In particular, its transparency has allowed for the use of reporter molecules to easily visualize dynamic processes at the organism, tissue, or cellular levels in a simple, non-invasive manner. In this thesis, we characterized and modeled one such processes in C. elegans, the heat shock response, using the reporter protein Green Fluorescent Protein (GFP). The heat shock response is an ancient and highly conserved process that responds to changes in the homeostasis of the proteome due to outside stressors such as temperature increases, oxidative stress, heavy metals, and other environmental stresses. Protection of protein homeostasis is fundamental for preserving cellular processes and ensuring both cell and organism health. However, protein homeostasis is challenged as the organism ages, as the capacity to maintain homeostasis decreases while the amount of protein misfolding and aggregation increases. Herein, we quantified the time-course expression of the small heat shock protein HSP-16.2 via GFP fluorescence in isogenic TJ375 [HSP-16.2"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Analysis of Compartmental Dynamics of Heat Shock Response in Caenorhabditis elegans"]}]}],"canonical_facts":{"dc:contributor":["Gunawan, Rudiyanto","Chemical and Biological Engineering"],"dc:creator":["Annis, Jillian"],"dc:date":["2019-10-29T16:48:14Z","2019","2019-08-08 15:56:51"],"dc:description":["M.S.","Caenorhabditis elegans is one of the most widely used model organisms in biological research. Its innate characteristics such as small size, transparency, and high degree of genetic homology with humans in conjunction with the large community dedicated to its study help make it an important model for studying highly conserved biological processes. In particular, its transparency has allowed for the use of reporter molecules to easily visualize dynamic processes at the organism, tissue, or cellular levels in a simple, non-invasive manner. In this thesis, we characterized and modeled one such processes in C. elegans, the heat shock response, using the reporter protein Green Fluorescent Protein (GFP). The heat shock response is an ancient and highly conserved process that responds to changes in the homeostasis of the proteome due to outside stressors such as temperature increases, oxidative stress, heavy metals, and other environmental stresses. Protection of protein homeostasis is fundamental for preserving cellular processes and ensuring both cell and organism health. However, protein homeostasis is challenged as the organism ages, as the capacity to maintain homeostasis decreases while the amount of protein misfolding and aggregation increases. Herein, we quantified the time-course expression of the small heat shock protein HSP-16.2 via GFP fluorescence in isogenic TJ375 [HSP-16.2"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80928"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["molecular biology","aging"],"dc:title":["Analysis of Compartmental Dynamics of Heat Shock Response in Caenorhabditis elegans"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:28Z"}