{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80908"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80908","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Role of Mechanosensitive Ion Channel Piezo1 in Detection of Confinements at ECM","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Bahrani Fard, Mohammad Reza"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Hua, Susan","Mechanical and Aerospace Engineering"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-29T16:48:03Z","date_published":"2019-10-29T16:48:03Z","updated_at":"2026-07-27T19:05:25Z","subjects":["mechanical engineering","bioengineering"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80908","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hua, Susan","Mechanical and Aerospace Engineering"]},{"key":"dc:creator","label":"Author","values":["Bahrani Fard, Mohammad Reza"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-29T16:48:03Z","2019","2019-08-07 16:13:42"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["mechanical engineering","bioengineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80908"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Cellular response to confinement at the extracellular matrix (ECM) plays an important role during tissue development. Mishandling these mechanical signals results in a variety of diseases including cardiovascular disease and cancer. In this study, we investigate the role of mechanosensitive Piezo1 channel as a cell sensor for detection of mechanical cues at various substrates. We modified the substrates with various micropatterns, topographies, and stiffness and examined the cell shape in response to ECM modifications. By inhibiting Piezo1 activity with specific pharmacologic inhibitors or by knockout of Piezo1 with siRNA, we have shown for the first time that HEK cells lost their ability to stretch to the full extent on the stripe patterns compared with wild-type cells. This indicates that cells utilize Piezo1 channels for detection of substrate confinements. The location and concentration of Piezo1 channels were visualized using FRET-based fluorescent probes P1-cpstFRET. We show that the distribution of Piezo1 is non-uniform in cells on narrow stripes, and Piezo1 clusters exist in areas of high cytoskeletal tension. This work offers a new mechanism of mechanosensing by Piezo1 channels, through which cells utilize ECM cues for remodeling."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of Mechanosensitive Ion Channel Piezo1 in Detection of Confinements at ECM"]}]}],"canonical_facts":{"dc:contributor":["Hua, Susan","Mechanical and Aerospace Engineering"],"dc:creator":["Bahrani Fard, Mohammad Reza"],"dc:date":["2019-10-29T16:48:03Z","2019","2019-08-07 16:13:42"],"dc:description":["M.S.","Cellular response to confinement at the extracellular matrix (ECM) plays an important role during tissue development. Mishandling these mechanical signals results in a variety of diseases including cardiovascular disease and cancer. In this study, we investigate the role of mechanosensitive Piezo1 channel as a cell sensor for detection of mechanical cues at various substrates. We modified the substrates with various micropatterns, topographies, and stiffness and examined the cell shape in response to ECM modifications. By inhibiting Piezo1 activity with specific pharmacologic inhibitors or by knockout of Piezo1 with siRNA, we have shown for the first time that HEK cells lost their ability to stretch to the full extent on the stripe patterns compared with wild-type cells. This indicates that cells utilize Piezo1 channels for detection of substrate confinements. The location and concentration of Piezo1 channels were visualized using FRET-based fluorescent probes P1-cpstFRET. We show that the distribution of Piezo1 is non-uniform in cells on narrow stripes, and Piezo1 clusters exist in areas of high cytoskeletal tension. This work offers a new mechanism of mechanosensing by Piezo1 channels, through which cells utilize ECM cues for remodeling."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80908"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["mechanical engineering","bioengineering"],"dc:title":["The Role of Mechanosensitive Ion Channel Piezo1 in Detection of Confinements at ECM"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:25Z"}