{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80894"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80894","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Characterization of Human Colonic Smooth Muscle Cells","abstract":"M.A.","abstract_html":"M.A.","abstract_has_math":false,"creators":["Hoxha, Din"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Duffey, Michael","Physiology and Biophysics"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-29T16:47:56Z","date_published":"2019-10-29T16:47:56Z","updated_at":"2026-07-27T19:05:25Z","subjects":["physiology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80894","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Duffey, Michael","Physiology and Biophysics"]},{"key":"dc:creator","label":"Author","values":["Hoxha, Din"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-29T16:47:56Z","2019","2019-08-08 18:32:37"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80894"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.A.","Smooth muscle of the gastrointestinal tract (visceral smooth muscle) propels and mixes food to facilitate digestion and absorption of nutrients. Similar to skeletal muscle, the contraction of visceral smooth muscle cells is caused by the sliding of actin and myosin filaments activated by a rise in intracellular Ca2+. The source of Ca2+ in visceral smooth muscle is extracellular since these cells do not have extensive Ca2+ stores. Extracellular Ca2+ enters via voltage-dependent Ca2+ channels (VDCC) and the VDCC of interest in visceral SMCs is CaV1.2. A primary cell line derived from human colon named Human Colonic Smooth Muscle Cells (HCoSMCs) has been developed. We characterized HCoSMCs to determine if these cells constitute a valid model for physiological studies of visceral smooth muscle function. The main hypothesis of this work is that HCoSMCs conserves the profile of differentiated visceral smooth muscle for several days and exhibits normal behavior pertaining to Ca2+ handling. We used fluorescent antibodies to smoothelin (an intracellular protein marker for smooth muscle differentiation) and fluorescent antibodies to CaV1.2, to determine if these cells are differentiated smooth muscle cells. We also used the fluorescent intracellular Ca2+ indicator, Fura-2AM, to determine if CaV1.2 functions correctly in these cells. Our data shows that HCoSMCs in culture retain a differentiated muscle phenotype with respect to expression of smoothelin and CaV1.2. In addition, we show that CaV1.2 is a functional voltage-gated Ca2+ channel in these cells. Finally, we show that Ca2+ handling in HCoSMCs is normal with respect to stimulation by the neurotransmitters adenosine triphosphate and acetylcholine (mimicked by carbachol). Thus, HCoSMCs are a viable isolated primary cell culture preparation that can be used as a model for the study of electrophysiological properties of CaV1.2 and Ca2+ handling in visceral smooth muscle."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Characterization of Human Colonic Smooth Muscle Cells"]}]}],"canonical_facts":{"dc:contributor":["Duffey, Michael","Physiology and Biophysics"],"dc:creator":["Hoxha, Din"],"dc:date":["2019-10-29T16:47:56Z","2019","2019-08-08 18:32:37"],"dc:description":["M.A.","Smooth muscle of the gastrointestinal tract (visceral smooth muscle) propels and mixes food to facilitate digestion and absorption of nutrients. Similar to skeletal muscle, the contraction of visceral smooth muscle cells is caused by the sliding of actin and myosin filaments activated by a rise in intracellular Ca2+. The source of Ca2+ in visceral smooth muscle is extracellular since these cells do not have extensive Ca2+ stores. Extracellular Ca2+ enters via voltage-dependent Ca2+ channels (VDCC) and the VDCC of interest in visceral SMCs is CaV1.2. A primary cell line derived from human colon named Human Colonic Smooth Muscle Cells (HCoSMCs) has been developed. We characterized HCoSMCs to determine if these cells constitute a valid model for physiological studies of visceral smooth muscle function. The main hypothesis of this work is that HCoSMCs conserves the profile of differentiated visceral smooth muscle for several days and exhibits normal behavior pertaining to Ca2+ handling. We used fluorescent antibodies to smoothelin (an intracellular protein marker for smooth muscle differentiation) and fluorescent antibodies to CaV1.2, to determine if these cells are differentiated smooth muscle cells. We also used the fluorescent intracellular Ca2+ indicator, Fura-2AM, to determine if CaV1.2 functions correctly in these cells. Our data shows that HCoSMCs in culture retain a differentiated muscle phenotype with respect to expression of smoothelin and CaV1.2. In addition, we show that CaV1.2 is a functional voltage-gated Ca2+ channel in these cells. Finally, we show that Ca2+ handling in HCoSMCs is normal with respect to stimulation by the neurotransmitters adenosine triphosphate and acetylcholine (mimicked by carbachol). Thus, HCoSMCs are a viable isolated primary cell culture preparation that can be used as a model for the study of electrophysiological properties of CaV1.2 and Ca2+ handling in visceral smooth muscle."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80894"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["physiology"],"dc:title":["Characterization of Human Colonic Smooth Muscle Cells"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:25Z"}