{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80845"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80845","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Role of Triacylglycerols and Lipid Droplets During Apoptosis","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Li-Purcell, Nasi"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Atilla-Gokcumen, G. Ekin","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-29T16:47:29Z","date_published":"2019-10-29T16:47:29Z","updated_at":"2026-07-27T19:05:25Z","subjects":["chemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80845","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Atilla-Gokcumen, G. Ekin","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Li-Purcell, Nasi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-29T16:47:29Z","2019","2019-07-11 14:33:35"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80845"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Apoptosis, a form of programmed cell death, is an essential cellular process. Deregulation of apoptosis is linked to many diseases, including cancer. Many efforts have been dedicated in understanding the molecular mechanism involved in apoptosis. The majority of these efforts focused on proteins; however, the involvement of lipids is still poorly understood. Thus, the aim of this study is to investigate the functional involvement of specific lipid structures and their regulation in apoptosis. As a first step to understand the changes in lipids at the molecular level during apoptosis, we utilized mass-spectrometry (MS)-based untargeted lipidomics and compared the lipidome of control and 5-fluorouracil-treated apoptotic HCT-116 cells. We found that TAGs containing polyunsaturated fatty acyl chains (PUFA-TAGs) show profound accumulation in 5-fluorouracil-treated apoptotic cells. We then induced apoptosis using other apoptotic inducers (i.e. staurosporine and doxorubicin) in HCT-116 cells and another cell line (MCF-7 cells), and we observed PUFA-TAG accumulation in these conditions as well. Our data suggest that PUFA-TAG accumulation can be generalized to apoptosis. To understand the enzymatic regulation behind the lipid changes we observed, we studied the key enzymes involved in TAG biosynthesis. We found that diacylglycerol acyltransferases, the enzymes catalyze the last step of TAG biosynthesis, are activated during apoptosis, suggesting the observed TAG accumulation during apoptosis could be due to the activation of these enzymes. Next, we studied the localization of these accumulated PUFA-TAGs during apoptosis. Our results suggest that these lipids are stored in lipid droplets, endoplasmic reticulum derived structures. Since membrane-residing PUFA-phospholipids can undergo oxidation and form reactive species under increased oxidative stress, we hypothesized that incorporation of PUFAs to PUFA-TAGs and their localization within lipid droplets during apoptosis limits the toxicity during this process."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of Triacylglycerols and Lipid Droplets During Apoptosis"]}]}],"canonical_facts":{"dc:contributor":["Atilla-Gokcumen, G. Ekin","Chemistry"],"dc:creator":["Li-Purcell, Nasi"],"dc:date":["2019-10-29T16:47:29Z","2019","2019-07-11 14:33:35"],"dc:description":["Ph.D.","Apoptosis, a form of programmed cell death, is an essential cellular process. Deregulation of apoptosis is linked to many diseases, including cancer. Many efforts have been dedicated in understanding the molecular mechanism involved in apoptosis. The majority of these efforts focused on proteins; however, the involvement of lipids is still poorly understood. Thus, the aim of this study is to investigate the functional involvement of specific lipid structures and their regulation in apoptosis. As a first step to understand the changes in lipids at the molecular level during apoptosis, we utilized mass-spectrometry (MS)-based untargeted lipidomics and compared the lipidome of control and 5-fluorouracil-treated apoptotic HCT-116 cells. We found that TAGs containing polyunsaturated fatty acyl chains (PUFA-TAGs) show profound accumulation in 5-fluorouracil-treated apoptotic cells. We then induced apoptosis using other apoptotic inducers (i.e. staurosporine and doxorubicin) in HCT-116 cells and another cell line (MCF-7 cells), and we observed PUFA-TAG accumulation in these conditions as well. Our data suggest that PUFA-TAG accumulation can be generalized to apoptosis. To understand the enzymatic regulation behind the lipid changes we observed, we studied the key enzymes involved in TAG biosynthesis. We found that diacylglycerol acyltransferases, the enzymes catalyze the last step of TAG biosynthesis, are activated during apoptosis, suggesting the observed TAG accumulation during apoptosis could be due to the activation of these enzymes. Next, we studied the localization of these accumulated PUFA-TAGs during apoptosis. Our results suggest that these lipids are stored in lipid droplets, endoplasmic reticulum derived structures. Since membrane-residing PUFA-phospholipids can undergo oxidation and form reactive species under increased oxidative stress, we hypothesized that incorporation of PUFAs to PUFA-TAGs and their localization within lipid droplets during apoptosis limits the toxicity during this process."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80845"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["chemistry"],"dc:title":["The Role of Triacylglycerols and Lipid Droplets During Apoptosis"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:25Z"}