{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80634"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80634","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Development and Characterization of Colloidal Gels for Osteogenic Differentiation of Mesenchymal Stem Cells","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Vadali, Bala Gopala Krishna; 0000-0002-4444-0425"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Sarkar, Debanjan","Biomedical Engineering"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-10-28T20:59:57Z","date_published":"2019-10-28T20:59:57Z","updated_at":"2026-07-27T19:05:23Z","subjects":["biomedical engineering"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80634","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sarkar, Debanjan","Biomedical Engineering"]},{"key":"dc:creator","label":"Author","values":["Vadali, Bala Gopala Krishna; 0000-0002-4444-0425"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-10-28T20:59:57Z","2019","2019-08-09 10:54:50"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biomedical engineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80634"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Colloidal gels are promising biomaterials which can exhibit a wide range of morphological and viscoelastic properties due to the self-oriented 3D networks of aggregated colloidal particles. Depending on the mode of aggregation, the networks of aggregated particles in the gels are developed in a spatially controlled manner to regulate the mechanomorphological characteristics. Thus, these gels can serve as a 3D matrix for controlling cell activity and regulating their responses. Specifically, ionic colloidal particles can be aggregated by electrostatic interactions with different particle fractions and the electrolyte concentration to form colloidal gels with defined mechanomorphology. We aim to design polyurethane (PU) based colloidal gels developed from anionic PU colloidal particles aggregated by divalent calcium ion as cationic electrolyte and use the calcium mediated PU based colloidal gels for modulating the mesenchymal stem cell (MSC) responses toward osteogenic differentiation. These colloidal gels, owing to their mechanomorphological character, can regulate the human MSCs toward osteogenic differentiation and the calcium mediated matrix can serve as a template for mineralization. In this thesis, three calcium mediated PU based colloidal gels were developed by aggregating PU colloids using three different concentrations of calcium as electrolyte and their physiochemical and mechanomorphological characteristics were analyzed. The gels showed distinctly different microstructural morphology and viscoelastic character according to the spatial mode of particle organization. MSCs differentiated in these gel matrices in a differential manner as evidenced from the upregulation of markers and deposition of matrix corresponding to osteogenic lineage. Overall, these colloidal gels show the potential of osteogenic matrix for bone regeneration. In summary, these colloidal gels can be engineered as 3D matrices with defined mechanomorphological properties which can guide the stem cells during their morphogenesis and differentiation."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development and Characterization of Colloidal Gels for Osteogenic Differentiation of Mesenchymal Stem Cells"]}]}],"canonical_facts":{"dc:contributor":["Sarkar, Debanjan","Biomedical Engineering"],"dc:creator":["Vadali, Bala Gopala Krishna; 0000-0002-4444-0425"],"dc:date":["2019-10-28T20:59:57Z","2019","2019-08-09 10:54:50"],"dc:description":["M.S.","Colloidal gels are promising biomaterials which can exhibit a wide range of morphological and viscoelastic properties due to the self-oriented 3D networks of aggregated colloidal particles. Depending on the mode of aggregation, the networks of aggregated particles in the gels are developed in a spatially controlled manner to regulate the mechanomorphological characteristics. Thus, these gels can serve as a 3D matrix for controlling cell activity and regulating their responses. Specifically, ionic colloidal particles can be aggregated by electrostatic interactions with different particle fractions and the electrolyte concentration to form colloidal gels with defined mechanomorphology. We aim to design polyurethane (PU) based colloidal gels developed from anionic PU colloidal particles aggregated by divalent calcium ion as cationic electrolyte and use the calcium mediated PU based colloidal gels for modulating the mesenchymal stem cell (MSC) responses toward osteogenic differentiation. These colloidal gels, owing to their mechanomorphological character, can regulate the human MSCs toward osteogenic differentiation and the calcium mediated matrix can serve as a template for mineralization. In this thesis, three calcium mediated PU based colloidal gels were developed by aggregating PU colloids using three different concentrations of calcium as electrolyte and their physiochemical and mechanomorphological characteristics were analyzed. The gels showed distinctly different microstructural morphology and viscoelastic character according to the spatial mode of particle organization. MSCs differentiated in these gel matrices in a differential manner as evidenced from the upregulation of markers and deposition of matrix corresponding to osteogenic lineage. Overall, these colloidal gels show the potential of osteogenic matrix for bone regeneration. In summary, these colloidal gels can be engineered as 3D matrices with defined mechanomorphological properties which can guide the stem cells during their morphogenesis and differentiation."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80634"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["biomedical engineering"],"dc:title":["Development and Characterization of Colloidal Gels for Osteogenic Differentiation of Mesenchymal Stem Cells"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:23Z"}