{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/80049"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/80049","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Protein Arginine Methylation Facilitates Environmental Adaptation in the Budding Yeast Saccharomyces cerevisiae","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Bijoo Davis, Richoo"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Yu, Michael","Biological Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:12:06Z","date_published":"2019-07-30T15:12:06Z","updated_at":"2026-07-27T19:05:23Z","subjects":["molecular biology","biochemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/80049","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Yu, Michael","Biological Sciences"]},{"key":"dc:creator","label":"Author","values":["Bijoo Davis, Richoo"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:12:06Z","2019","2019-05-19 20:23:03"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["molecular biology","biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/80049"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Cells need to adapt to a constantly changing environment in order to survive and thrive. They are able to do so by continually modulating their gene expression profile to meet challenges posed by these environmental stressors. While there are multiple stages at which cells can regulate their gene expression, regulation at the level of transcription is the most common mode. Despite much work devoted to understanding the fundamental switches and mechanisms underlying transcriptional regulation, many of the nuances in transcriptional regulation are not well understood, and scientists have only begun to appreciate the intricate details that formulate the molecular basis for fine-tuning transcriptional output. My work described here reveals a novel role for the budding yeast protein arginine methyltransferase enzyme, Hmt1, in fine-tuning the transcription of tRNA genes in response to stress. Hmt1 methylates the RNA Pol III specific subunit Rpc31, wherein the methyl marks promote interaction between Pol III and its repressor Maf1 and thereby facilitates the repression of Pol III transcription in response to stress. We have also identified a role for Hmt1 in the adaptation of yeast cells to fermentable carbon sources. As a survival strategy, yeast cells prefer the use of fermentation over respiration when presented with abundance of glucose and oxygen. Hmt1 promotes this strategy by suppressing respiration. Additionally, in the presence of abundant glucose when yeast cells carry out fermentation, Hmt1 suppresses the expression of many genes associated with mitochondrial functioning that promote respiration. This reduction in expression could decrease the abundance of proteins required for respiratory growth and accordingly increase the glycolytic flux through the fermentation pathway that provides a competitive advantage for the budding yeast in its natural habitat.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Protein Arginine Methylation Facilitates Environmental Adaptation in the Budding Yeast Saccharomyces cerevisiae"]}]}],"canonical_facts":{"dc:contributor":["Yu, Michael","Biological Sciences"],"dc:creator":["Bijoo Davis, Richoo"],"dc:date":["2019-07-30T15:12:06Z","2019","2019-05-19 20:23:03"],"dc:description":["Ph.D.","Cells need to adapt to a constantly changing environment in order to survive and thrive. They are able to do so by continually modulating their gene expression profile to meet challenges posed by these environmental stressors. While there are multiple stages at which cells can regulate their gene expression, regulation at the level of transcription is the most common mode. Despite much work devoted to understanding the fundamental switches and mechanisms underlying transcriptional regulation, many of the nuances in transcriptional regulation are not well understood, and scientists have only begun to appreciate the intricate details that formulate the molecular basis for fine-tuning transcriptional output. My work described here reveals a novel role for the budding yeast protein arginine methyltransferase enzyme, Hmt1, in fine-tuning the transcription of tRNA genes in response to stress. Hmt1 methylates the RNA Pol III specific subunit Rpc31, wherein the methyl marks promote interaction between Pol III and its repressor Maf1 and thereby facilitates the repression of Pol III transcription in response to stress. We have also identified a role for Hmt1 in the adaptation of yeast cells to fermentable carbon sources. As a survival strategy, yeast cells prefer the use of fermentation over respiration when presented with abundance of glucose and oxygen. Hmt1 promotes this strategy by suppressing respiration. Additionally, in the presence of abundant glucose when yeast cells carry out fermentation, Hmt1 suppresses the expression of many genes associated with mitochondrial functioning that promote respiration. This reduction in expression could decrease the abundance of proteins required for respiratory growth and accordingly increase the glycolytic flux through the fermentation pathway that provides a competitive advantage for the budding yeast in its natural habitat.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/80049"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["molecular biology","biochemistry"],"dc:title":["Protein Arginine Methylation Facilitates Environmental Adaptation in the Budding Yeast Saccharomyces cerevisiae"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:23Z"}