{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79957"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79957","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Streptococcus gordonii Binding to Dental Biofilm Components: Role of Different Adhesins","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Alsantli, Maram"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Ruhl, Stefan","Oral Biology"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:11:24Z","date_published":"2019-07-30T15:11:24Z","updated_at":"2026-07-27T19:05:21Z","subjects":["dentistry","microbiology","biochemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79957","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ruhl, Stefan","Oral Biology"]},{"key":"dc:creator","label":"Author","values":["Alsantli, Maram"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:11:24Z","2019","2019-05-15 16:16:55"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry","microbiology","biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79957"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Objectives: Dental biofilm formation is initiated by the adsorption of salivary components to the tooth surface, thereby facilitating the attachment of oral bacterial pioneer colonizers. Streptococcus gordonii is one of the bacterial species that inhabit the early dental biofilm. It expresses multiple surface adhesins believed to aid in its attachment to surfaces in the oral cavity as well as to other organisms in the dental biofilm. One well-characterized adhesin, named Hsa, binds to sialic-acid-containing glycans on salivary mucins and other host glycoproteins. There is evidence that S. gordonii expresses additional surface proteins involved in adhesion. Our goal here was to screen for additional putative surface-anchored adhesins that might facilitate S. gordonii binding to dental biofilm components. Methods: Derivatives of S. gordonii strain Challis DL1 were constructed with deletions of genes encoding proteins with conserved functional domains that could play a role in adhesion to dental biofilm components. Using the bacterial overlay method, we investigated the binding of a panel of eleven isogenic mutant strains to immobilized samples of proteins, glycoproteins, parotid saliva, submandibular-sublingual (SMSL) saliva, and whole saliva in addition to an S. oralis glycan receptor. Additionally, we evaluated the influence of divalent cations on bacterial binding. Results: Compared to its parental strain, the binding of the Hsa-deficient mutant to parotid saliva and the S. oralis glycan receptor was not affected. This suggests that adhesins other than Hsa play a role in S. gordonii attachment to components in the dental biofilm. Only four mutants showed varying binding activities compared to the parental strain. In the absence of divalent cations, binding to all three saliva samples as well as the S. oralis glycan receptor did not occur. This suggests that those binding activities of S. gordonii are calcium-ion-dependent. Conclusion: We could demonstrate that the bacterial overlay method is well suited to screen our available collection of S. gordonii mutant strains for the presence of additionalputative adhesins that mediate binding to dental biofilm components."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Streptococcus gordonii Binding to Dental Biofilm Components: Role of Different Adhesins"]}]}],"canonical_facts":{"dc:contributor":["Ruhl, Stefan","Oral Biology"],"dc:creator":["Alsantli, Maram"],"dc:date":["2019-07-30T15:11:24Z","2019","2019-05-15 16:16:55"],"dc:description":["M.S.","Objectives: Dental biofilm formation is initiated by the adsorption of salivary components to the tooth surface, thereby facilitating the attachment of oral bacterial pioneer colonizers. Streptococcus gordonii is one of the bacterial species that inhabit the early dental biofilm. It expresses multiple surface adhesins believed to aid in its attachment to surfaces in the oral cavity as well as to other organisms in the dental biofilm. One well-characterized adhesin, named Hsa, binds to sialic-acid-containing glycans on salivary mucins and other host glycoproteins. There is evidence that S. gordonii expresses additional surface proteins involved in adhesion. Our goal here was to screen for additional putative surface-anchored adhesins that might facilitate S. gordonii binding to dental biofilm components. Methods: Derivatives of S. gordonii strain Challis DL1 were constructed with deletions of genes encoding proteins with conserved functional domains that could play a role in adhesion to dental biofilm components. Using the bacterial overlay method, we investigated the binding of a panel of eleven isogenic mutant strains to immobilized samples of proteins, glycoproteins, parotid saliva, submandibular-sublingual (SMSL) saliva, and whole saliva in addition to an S. oralis glycan receptor. Additionally, we evaluated the influence of divalent cations on bacterial binding. Results: Compared to its parental strain, the binding of the Hsa-deficient mutant to parotid saliva and the S. oralis glycan receptor was not affected. This suggests that adhesins other than Hsa play a role in S. gordonii attachment to components in the dental biofilm. Only four mutants showed varying binding activities compared to the parental strain. In the absence of divalent cations, binding to all three saliva samples as well as the S. oralis glycan receptor did not occur. This suggests that those binding activities of S. gordonii are calcium-ion-dependent. Conclusion: We could demonstrate that the bacterial overlay method is well suited to screen our available collection of S. gordonii mutant strains for the presence of additionalputative adhesins that mediate binding to dental biofilm components."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79957"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry","microbiology","biochemistry"],"dc:title":["Streptococcus gordonii Binding to Dental Biofilm Components: Role of Different Adhesins"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:21Z"}