{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79952"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79952","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Comparison of Titanium Disinfection Methods","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Forgo, Gyula"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Haraszthy, Violet","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:11:21Z","date_published":"2019-07-30T15:11:21Z","updated_at":"2026-07-27T19:05:21Z","subjects":["dentistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79952","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Haraszthy, Violet","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Forgo, Gyula"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:11:21Z","2019","2019-05-15 14:46:34"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79952"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Objective: Peri-implantitis results from microbial infection of dental implants. This in vitro study compared the efficacy of different viability assays and treatment modalities in eliminating biofilm formed on commercially pure titanium disks. Methods: Smooth surface titanium disks, uniformly 10mmx1mm (Ra<0.2m, Grade: G2, ESPI metals Q13279) were sterilely placed into 10 ml of brain-heart infusion (BHI, Difco Laboratories, Detroit, MI, USA) broth mixed with 1 mL of collected saliva from volunteers. The titanium disks were incubated for 72 hours at 37°C in this mixture. The disks were removed, gently rinsed to remove non-adherent microorganisms and treated with either; 1) dental curette; 2) sandblaster (Microetcher®-Danville Materials); 3) titanium brush (Straumann® TiBrush); 4) implantoplasty; 5) diode laser on 1 W (Odyssey, Ivoclar); 6) 1% citric acid for one minute. All treatments were performed in triplicate. From each treatment group, three specimens were 1) cultured in BHI liquid media for 24h at 37oC, then 0.1 ml aliquots of different dilutions were distributed on blood agar media and anaerobically incubated for 72 hours at 37oC; 2) placed in 10 ml of Ringers solution, sonicated and vortexed for 60 seconds, then plated undiluted on blood agar and anaerobically incubated for 72 hours at 37oC; 3) examined with an adenosine triphosphate (ATP) determination kit by Molecular Probes®; 4) assessed with an on-site adenosine triphosphate determination kit (Ultrasnap ATP Swab with Hygiena Luminometer®); 5) examined with ToxinSensor Chromogenic Limulus Amebocyte Lysate (LAL) Endotoxin Assay Kit® (GenScript®). Untreated disks were included in the study as controls.Results: Compared to positive controls the biofilm, that remained on the titanium discs after treatment showed a great reduction. Titanium curette treatment group proved to be the least efficient. Endotoxins proved to be harder to remove, however air abrasive treatment and implantoplasty showed a greater reduction in the remaining lipopolysaccharide numbers. Conclusions: Bacteriological, ATP and endotoxin examinations show that among our treatment groups implantoplasty and air abrasive treatments were the most efficient in the disinfection of titanium surfaces. Molecular Probes®-ATP Luminescence Assay seems to be the most sensitive assay assessing microbial viability on titanium surfaces."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparison of Titanium Disinfection Methods"]}]}],"canonical_facts":{"dc:contributor":["Haraszthy, Violet","Oral Sciences"],"dc:creator":["Forgo, Gyula"],"dc:date":["2019-07-30T15:11:21Z","2019","2019-05-15 14:46:34"],"dc:description":["M.S.","Objective: Peri-implantitis results from microbial infection of dental implants. This in vitro study compared the efficacy of different viability assays and treatment modalities in eliminating biofilm formed on commercially pure titanium disks. Methods: Smooth surface titanium disks, uniformly 10mmx1mm (Ra<0.2m, Grade: G2, ESPI metals Q13279) were sterilely placed into 10 ml of brain-heart infusion (BHI, Difco Laboratories, Detroit, MI, USA) broth mixed with 1 mL of collected saliva from volunteers. The titanium disks were incubated for 72 hours at 37°C in this mixture. The disks were removed, gently rinsed to remove non-adherent microorganisms and treated with either; 1) dental curette; 2) sandblaster (Microetcher®-Danville Materials); 3) titanium brush (Straumann® TiBrush); 4) implantoplasty; 5) diode laser on 1 W (Odyssey, Ivoclar); 6) 1% citric acid for one minute. All treatments were performed in triplicate. From each treatment group, three specimens were 1) cultured in BHI liquid media for 24h at 37oC, then 0.1 ml aliquots of different dilutions were distributed on blood agar media and anaerobically incubated for 72 hours at 37oC; 2) placed in 10 ml of Ringers solution, sonicated and vortexed for 60 seconds, then plated undiluted on blood agar and anaerobically incubated for 72 hours at 37oC; 3) examined with an adenosine triphosphate (ATP) determination kit by Molecular Probes®; 4) assessed with an on-site adenosine triphosphate determination kit (Ultrasnap ATP Swab with Hygiena Luminometer®); 5) examined with ToxinSensor Chromogenic Limulus Amebocyte Lysate (LAL) Endotoxin Assay Kit® (GenScript®). Untreated disks were included in the study as controls.Results: Compared to positive controls the biofilm, that remained on the titanium discs after treatment showed a great reduction. Titanium curette treatment group proved to be the least efficient. Endotoxins proved to be harder to remove, however air abrasive treatment and implantoplasty showed a greater reduction in the remaining lipopolysaccharide numbers. Conclusions: Bacteriological, ATP and endotoxin examinations show that among our treatment groups implantoplasty and air abrasive treatments were the most efficient in the disinfection of titanium surfaces. Molecular Probes®-ATP Luminescence Assay seems to be the most sensitive assay assessing microbial viability on titanium surfaces."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79952"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry"],"dc:title":["Comparison of Titanium Disinfection Methods"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:21Z"}