{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79924"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79924","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Effect of Radio Frequency Glow-Discharge Treatment of Titanium on Human Gingival Fibroblasts as a Function of Distance","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Ali, Aya"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Meyer, Anne","Biomaterials"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:11:08Z","date_published":"2019-07-30T15:11:08Z","updated_at":"2026-07-27T19:05:21Z","subjects":["dentistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79924","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Meyer, Anne","Biomaterials"]},{"key":"dc:creator","label":"Author","values":["Ali, Aya"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:11:08Z","2019","2019-05-13 14:06:46"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79924"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Abstract Radio frequency glow discharge (RFGD) treatment has been used as a sterilization method in many studies. These studies showed greater attachment and spreading of fibroblasts over surfaces that had been sterilized using RFGD. One in vitro study found that this effect is not only exclusive to the cells that come in contact with the treated materials, but affects the cells at a distance from these materials as well. In the current study, in vitro experiments were conducted to investigate the possible causes of this phenomenon. Contact angle measurements and infrared spectroscopy were done to investigate the differences on the surfaces of the bacterial grade polystyrene dishes used in the study. To investigate the effect of the treatment on the fibroblasts, two different cell culture experiments were done. Initially, fibroblasts were added to the dishes with commercially pure titanium (cpTi) specimens (control and RFGD treated) inside them. The second experiment was conducted using conditioned media transferred from a replicate of the first experiment to new bacterial grade dishes that did not contain cpTi. As a result of the RFGD process applied to cpTi foils, human gingival fibroblasts showed significantly higher attachment (greater numbers) and spreading (greater surface areas) on bacterial grade polystyrene dishes at a distance from the cpTi foils, in comparison to cells in dishes with the control cpTi foils, which were sterilized using UV light. Surface characterization of the polystyrene dishes, through contact angle measurements, showed a significant difference in the critical surface tensions between the two groups. Moreover, the difference in the contents of the medium from the two groups had great effect on the fibroblast attachment and spreading after transfer to new dishes. These results confirmed and supported the observations of previous studies that RFGD improved the attachment and spread of the fibroblasts at a distance from the treated cp titanium."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Effect of Radio Frequency Glow-Discharge Treatment of Titanium on Human Gingival Fibroblasts as a Function of Distance"]}]}],"canonical_facts":{"dc:contributor":["Meyer, Anne","Biomaterials"],"dc:creator":["Ali, Aya"],"dc:date":["2019-07-30T15:11:08Z","2019","2019-05-13 14:06:46"],"dc:description":["M.S.","Abstract Radio frequency glow discharge (RFGD) treatment has been used as a sterilization method in many studies. These studies showed greater attachment and spreading of fibroblasts over surfaces that had been sterilized using RFGD. One in vitro study found that this effect is not only exclusive to the cells that come in contact with the treated materials, but affects the cells at a distance from these materials as well. In the current study, in vitro experiments were conducted to investigate the possible causes of this phenomenon. Contact angle measurements and infrared spectroscopy were done to investigate the differences on the surfaces of the bacterial grade polystyrene dishes used in the study. To investigate the effect of the treatment on the fibroblasts, two different cell culture experiments were done. Initially, fibroblasts were added to the dishes with commercially pure titanium (cpTi) specimens (control and RFGD treated) inside them. The second experiment was conducted using conditioned media transferred from a replicate of the first experiment to new bacterial grade dishes that did not contain cpTi. As a result of the RFGD process applied to cpTi foils, human gingival fibroblasts showed significantly higher attachment (greater numbers) and spreading (greater surface areas) on bacterial grade polystyrene dishes at a distance from the cpTi foils, in comparison to cells in dishes with the control cpTi foils, which were sterilized using UV light. Surface characterization of the polystyrene dishes, through contact angle measurements, showed a significant difference in the critical surface tensions between the two groups. Moreover, the difference in the contents of the medium from the two groups had great effect on the fibroblast attachment and spreading after transfer to new dishes. These results confirmed and supported the observations of previous studies that RFGD improved the attachment and spread of the fibroblasts at a distance from the treated cp titanium."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79924"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry"],"dc:title":["Effect of Radio Frequency Glow-Discharge Treatment of Titanium on Human Gingival Fibroblasts as a Function of Distance"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:21Z"}