{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79921"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79921","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Unique Interactions in Ribosome Subunit Export in Atrican Trypanosomes","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Rink, Constance"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Williams, Noreen","Microbiology and Immunology"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:11:07Z","date_published":"2019-07-30T15:11:07Z","updated_at":"2026-07-27T19:05:21Z","subjects":["microbiology","parasitology","molecular biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79921","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Williams, Noreen","Microbiology and Immunology"]},{"key":"dc:creator","label":"Author","values":["Rink, Constance"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:11:07Z","2019","2019-05-13 11:29:12"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["microbiology","parasitology","molecular biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79921"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","African trypanosomiasis affects both humans and animals in Sub-Saharan Africa and is caused by the parasite Trypanosoma brucei. Approximately 60- 70 million people are at risk of contracting human African trypanosomiasis (HAT or African sleeping sickness), while over 60 million livestock are at risk of contracting animal trypanosomiasis (AAT or nagana). HAT is fatal if left untreated, although drug treatments, vector control, and other preventative measures have led to a decrease in mortality in recent years. Nagana (the animal trypanosomiasis) results in significant economic impact to the region with an estimated annual loss of $4.5 billion dollars. The current treatments are inadequate due to toxicity, cost, and drug resistance. Targeted development of new, more effective chemotherapeutics requires a better understanding of essential parasite-specific biological processes. Ribosome biogenesis, the formation of functional ribosomes, is a highly conserved and vital process for all living cells, including T. brucei. Ribosome assembly involves more than 80 ribosomal proteins, over 200 non-ribosomal proteins, and 4 species of ribosomal RNA (28S, 18S, 5.8S and 5S). A critical step in ribosome biogenesis is the transport of the pre-60S and pre-40S particles across the nuclear pore by the nuclear export complex (NEC), which includes the exportin 1 (Xpo1) and Nmd3 proteins. Additional auxiliary export receptors Mex67 and Mtr2 facilitate the transport of ribosomal subunits through the nuclear pore complex (NPC). As a heterodimer (Mex67-Mtr2) these proteins bind to the phenylalanine-glycine-rich repeat regions (FG) in the nucleoporins lining the nucleopore, thus allowing easy passage of the ribosomal subunits. In summary, we have identified several unique features of the trypanosome Mex67-Mtr2 auxiliary binding complex and have shown that it has a critical role in the formation of ribosomes and therefore, protein translation in these parasites. This work provides a foundation for future work that might target the trypanosome-specific features of the nuclear export complex for chemotherapeutic development.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Unique Interactions in Ribosome Subunit Export in Atrican Trypanosomes"]}]}],"canonical_facts":{"dc:contributor":["Williams, Noreen","Microbiology and Immunology"],"dc:creator":["Rink, Constance"],"dc:date":["2019-07-30T15:11:07Z","2019","2019-05-13 11:29:12"],"dc:description":["Ph.D.","African trypanosomiasis affects both humans and animals in Sub-Saharan Africa and is caused by the parasite Trypanosoma brucei. Approximately 60- 70 million people are at risk of contracting human African trypanosomiasis (HAT or African sleeping sickness), while over 60 million livestock are at risk of contracting animal trypanosomiasis (AAT or nagana). HAT is fatal if left untreated, although drug treatments, vector control, and other preventative measures have led to a decrease in mortality in recent years. Nagana (the animal trypanosomiasis) results in significant economic impact to the region with an estimated annual loss of $4.5 billion dollars. The current treatments are inadequate due to toxicity, cost, and drug resistance. Targeted development of new, more effective chemotherapeutics requires a better understanding of essential parasite-specific biological processes. Ribosome biogenesis, the formation of functional ribosomes, is a highly conserved and vital process for all living cells, including T. brucei. Ribosome assembly involves more than 80 ribosomal proteins, over 200 non-ribosomal proteins, and 4 species of ribosomal RNA (28S, 18S, 5.8S and 5S). A critical step in ribosome biogenesis is the transport of the pre-60S and pre-40S particles across the nuclear pore by the nuclear export complex (NEC), which includes the exportin 1 (Xpo1) and Nmd3 proteins. Additional auxiliary export receptors Mex67 and Mtr2 facilitate the transport of ribosomal subunits through the nuclear pore complex (NPC). As a heterodimer (Mex67-Mtr2) these proteins bind to the phenylalanine-glycine-rich repeat regions (FG) in the nucleoporins lining the nucleopore, thus allowing easy passage of the ribosomal subunits. In summary, we have identified several unique features of the trypanosome Mex67-Mtr2 auxiliary binding complex and have shown that it has a critical role in the formation of ribosomes and therefore, protein translation in these parasites. This work provides a foundation for future work that might target the trypanosome-specific features of the nuclear export complex for chemotherapeutic development.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79921"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["microbiology","parasitology","molecular biology"],"dc:title":["Unique Interactions in Ribosome Subunit Export in Atrican Trypanosomes"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:21Z"}