{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79911"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79911","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Dissection of the duf_vm Enhancer and Visceral Muscle Development in Drosophila melanogaster","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Delmont, Michael; 0000-0001-7352-0785"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Halfon, Marc","Genetics, Genomics and Bioinformatics"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:11:02Z","date_published":"2019-07-30T15:11:02Z","updated_at":"2026-07-27T19:05:21Z","subjects":["developmental biology","genetics","biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79911","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Halfon, Marc","Genetics, Genomics and Bioinformatics"]},{"key":"dc:creator","label":"Author","values":["Delmont, Michael; 0000-0001-7352-0785"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:11:02Z","2019","2019-05-10 14:50:39"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["developmental biology","genetics","biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79911"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","During development, undifferentiated cells will undergo cell fate specification and take on different roles. In Drosophila, two types of muscle cells, fusion-competent myoblasts (FCM) and founder cells (FC), are required for formation of final, syncytial muscle fibers. The FCs express a gene known as kirre/dumbfounded (duf), which codes for a cell-surface attractant for FCMs. As myoblast fusion is essential for proper muscle development in an organism and is prevented in the absence of duf, a proper understanding of the mechanisms regulating duf expression is particularly important. While signaling pathways in somatic and cardiac muscle are well characterized, visceral muscle has relatively little known about it at the transcriptional level. We previously identified a 700 bp functional enhancer for duf in the visceral muscle (duf_vm) inside of an intron and characterized it via mutagenesis of three ETS factor binding sites. This resulted in varying degrees of a knockdown of expression. Here, duf_vm has been further characterized. Using highly conserved regions as a guide, we subdivided the enhancer into ~200 bp segments. We created transgenic flies which have been used to determine the functional regions of the enhancer. Each segment was able to at least partially recapitulate the visceral mesoderm expression, suggesting redundancy in the enhancer. Two of the subdivisions showed decreased expression, while one region, named duf_vmB, appears to have full expression when compared to the original enhancer. Interestingly, this segment contains the ETS site which, when mutated in duf_vm, had the most severe phenotype and showed significantly decreased expression."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Dissection of the duf_vm Enhancer and Visceral Muscle Development in Drosophila melanogaster"]}]}],"canonical_facts":{"dc:contributor":["Halfon, Marc","Genetics, Genomics and Bioinformatics"],"dc:creator":["Delmont, Michael; 0000-0001-7352-0785"],"dc:date":["2019-07-30T15:11:02Z","2019","2019-05-10 14:50:39"],"dc:description":["M.S.","During development, undifferentiated cells will undergo cell fate specification and take on different roles. In Drosophila, two types of muscle cells, fusion-competent myoblasts (FCM) and founder cells (FC), are required for formation of final, syncytial muscle fibers. The FCs express a gene known as kirre/dumbfounded (duf), which codes for a cell-surface attractant for FCMs. As myoblast fusion is essential for proper muscle development in an organism and is prevented in the absence of duf, a proper understanding of the mechanisms regulating duf expression is particularly important. While signaling pathways in somatic and cardiac muscle are well characterized, visceral muscle has relatively little known about it at the transcriptional level. We previously identified a 700 bp functional enhancer for duf in the visceral muscle (duf_vm) inside of an intron and characterized it via mutagenesis of three ETS factor binding sites. This resulted in varying degrees of a knockdown of expression. Here, duf_vm has been further characterized. Using highly conserved regions as a guide, we subdivided the enhancer into ~200 bp segments. We created transgenic flies which have been used to determine the functional regions of the enhancer. Each segment was able to at least partially recapitulate the visceral mesoderm expression, suggesting redundancy in the enhancer. Two of the subdivisions showed decreased expression, while one region, named duf_vmB, appears to have full expression when compared to the original enhancer. Interestingly, this segment contains the ETS site which, when mutated in duf_vm, had the most severe phenotype and showed significantly decreased expression."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79911"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["developmental biology","genetics","biology"],"dc:title":["Dissection of the duf_vm Enhancer and Visceral Muscle Development in Drosophila melanogaster"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:21Z"}