{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79895"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79895","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Exploring the Role of Signaling Networks in Regulating Fungal Foraging Responses","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Chow, Jacky; 0000-0002-3849-0346"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Cullen, Paul","Biological Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-30T15:10:50Z","date_published":"2019-07-30T15:10:50Z","updated_at":"2026-07-27T19:05:19Z","subjects":["genetics"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79895","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cullen, Paul","Biological Sciences"]},{"key":"dc:creator","label":"Author","values":["Chow, Jacky; 0000-0002-3849-0346"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-30T15:10:50Z","2019","2019-05-07 07:57:30"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79895"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Yeast undergo morphological changes that can be critical for survival in response to environmental stresses. These stresses are sensed through various mechanisms that transduce information through a signaling network that regulates behaviors such as filamentous/invasive growth, biofilm/mat formation, and colony ruffling. While many studies tend to focus on the regulation and behavior of filamentous growth in the context of small groups of mother/daughter cells, many aspects of filamentous growth are not well understood. For example, yeast cells that undergo filamentous growth also produce a distinctive colony morphology, often referred to as colony ruffling. Both filamentous growth and colony ruffling are regulated by many of the same signaling pathways. However, it is not clear to what degree signaling pathway regulate filamentous growth and colony formation. Moreover, what benefit does colony ruffling give to yeast cells? In addition, how cells collectively undergo invasive growth in large numbers is not clear. In my thesis, we performed comparative RNA sequencing between the wild-type and signaling mutant colonies to identify critical mediators of filamentous behavior. We identified two major targets, FLO11 and SUC2 (Invertase), that played important roles in the development of filamentous growth at much larger scales than mother/daughter cells. Specifically, FLO11 and invertase were required for proper colony ruffling and the formation of invasive aggregates. We showed that Flo11p-mediated cell adhesion and colony ruffling allowed for evasion from nematode predators and allowed for more efficient heat dissipation when compared to non-adhesive, smooth colonies. We also the formation of invasive aggregates, which could invade 20x deeper than a filament, was dependent on FLO11 and invertase. Importantly, these large-scale behaviors were regulated by cell density, quorum sensing molecules, and the quorum sensing retrograde pathway. We explored functional relationships among pathways that shed light on cross-regulation within the filamentous growth signaling network. We examined the co-regulation of other important functions such as cell wall integrity and metabolic processes. Additionally, we identified important stresses that are unique to growth as a colony on solid media. Together these results elucidate often overlooked aspects of colony growth in yeast and the role of a filamentous growth signaling network in regulating these behaviors."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Exploring the Role of Signaling Networks in Regulating Fungal Foraging Responses"]}]}],"canonical_facts":{"dc:contributor":["Cullen, Paul","Biological Sciences"],"dc:creator":["Chow, Jacky; 0000-0002-3849-0346"],"dc:date":["2019-07-30T15:10:50Z","2019","2019-05-07 07:57:30"],"dc:description":["Ph.D.","Yeast undergo morphological changes that can be critical for survival in response to environmental stresses. These stresses are sensed through various mechanisms that transduce information through a signaling network that regulates behaviors such as filamentous/invasive growth, biofilm/mat formation, and colony ruffling. While many studies tend to focus on the regulation and behavior of filamentous growth in the context of small groups of mother/daughter cells, many aspects of filamentous growth are not well understood. For example, yeast cells that undergo filamentous growth also produce a distinctive colony morphology, often referred to as colony ruffling. Both filamentous growth and colony ruffling are regulated by many of the same signaling pathways. However, it is not clear to what degree signaling pathway regulate filamentous growth and colony formation. Moreover, what benefit does colony ruffling give to yeast cells? In addition, how cells collectively undergo invasive growth in large numbers is not clear. In my thesis, we performed comparative RNA sequencing between the wild-type and signaling mutant colonies to identify critical mediators of filamentous behavior. We identified two major targets, FLO11 and SUC2 (Invertase), that played important roles in the development of filamentous growth at much larger scales than mother/daughter cells. Specifically, FLO11 and invertase were required for proper colony ruffling and the formation of invasive aggregates. We showed that Flo11p-mediated cell adhesion and colony ruffling allowed for evasion from nematode predators and allowed for more efficient heat dissipation when compared to non-adhesive, smooth colonies. We also the formation of invasive aggregates, which could invade 20x deeper than a filament, was dependent on FLO11 and invertase. Importantly, these large-scale behaviors were regulated by cell density, quorum sensing molecules, and the quorum sensing retrograde pathway. We explored functional relationships among pathways that shed light on cross-regulation within the filamentous growth signaling network. We examined the co-regulation of other important functions such as cell wall integrity and metabolic processes. Additionally, we identified important stresses that are unique to growth as a colony on solid media. Together these results elucidate often overlooked aspects of colony growth in yeast and the role of a filamentous growth signaling network in regulating these behaviors."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79895"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["genetics"],"dc:title":["Exploring the Role of Signaling Networks in Regulating Fungal Foraging Responses"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:19Z"}