{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79814"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79814","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"LC-MS Based Lipidomics to Study the Lipids Involved in Barth Syndrome and the Lipid Composition of Different Serum Types","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Aldrich, Alyssa"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Atilla-Gokcumen, G. Ekin","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07-26T18:55:26Z","date_published":"2019-07-26T18:55:26Z","updated_at":"2026-07-27T19:05:19Z","subjects":["analytical chemistry","biochemistry","medicinal chemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79814","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Atilla-Gokcumen, G. Ekin","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Aldrich, Alyssa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07-26T18:55:26Z","2019","2019-05-13 13:50:11"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["analytical chemistry","biochemistry","medicinal chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79814"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Lipidomics is the study of lipids. Lipids have various biological functions ranging from signaling, providing energy or energy storage and making up the plasma and organelle membranes. The lipids that will be discussed in this thesis are fatty acids, glycerolipids, phospholipids, sphingolipids and sterols. The analytical technique used to analyze these lipids was liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry. Both targeted and untargeted analysis were utilized to study changes in the lipid levels. This thesis will discuss the differences between the lipidome of lymphoblast cell lines that were isolated and immortalized from patients with Barth syndrome and from healthy individuals. Along with the differences in lipids composition of three different types of serum used to supplement cell culture media. Barth syndrome is a genetic mutation on the TAZ gene which encodes for the enzyme Tafazzin. Tafazzin is an acyl transferase that is mainly responsible for transferring an acyl chain from a phosphatidylcholine or phosphatidylethanolamine to a monolysocardiolipin in vivo. This remodeling introduces other acyl chains in order to make a mature cardiolipin. These mature cardiolipins make up the mitochondrial membrane and play a role in the respiratory chain. Thus when there is an inactivating mutation on the TAZ gene, the production of these mature cardiolipins is impaired and the mitochondrial membrane loses integrity potentially leading to cell death. Untargeted analysis of lymphoblast cell lines isolated and immortalized from Barth patients compared to healthy patients showed ether lipids are affected: a monoether phosphatidylcholine accumulated and a monoether phosphatidylethanolamine depleted. Targeted analysis showed that phospholipids, for example phosphatidylglycerols and phosphatidylethanolamines accumulated. These observations along with others are outlined in Chapter 2."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["LC-MS Based Lipidomics to Study the Lipids Involved in Barth Syndrome and the Lipid Composition of Different Serum Types"]}]}],"canonical_facts":{"dc:contributor":["Atilla-Gokcumen, G. Ekin","Chemistry"],"dc:creator":["Aldrich, Alyssa"],"dc:date":["2019-07-26T18:55:26Z","2019","2019-05-13 13:50:11"],"dc:description":["M.S.","Lipidomics is the study of lipids. Lipids have various biological functions ranging from signaling, providing energy or energy storage and making up the plasma and organelle membranes. The lipids that will be discussed in this thesis are fatty acids, glycerolipids, phospholipids, sphingolipids and sterols. The analytical technique used to analyze these lipids was liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry. Both targeted and untargeted analysis were utilized to study changes in the lipid levels. This thesis will discuss the differences between the lipidome of lymphoblast cell lines that were isolated and immortalized from patients with Barth syndrome and from healthy individuals. Along with the differences in lipids composition of three different types of serum used to supplement cell culture media. Barth syndrome is a genetic mutation on the TAZ gene which encodes for the enzyme Tafazzin. Tafazzin is an acyl transferase that is mainly responsible for transferring an acyl chain from a phosphatidylcholine or phosphatidylethanolamine to a monolysocardiolipin in vivo. This remodeling introduces other acyl chains in order to make a mature cardiolipin. These mature cardiolipins make up the mitochondrial membrane and play a role in the respiratory chain. Thus when there is an inactivating mutation on the TAZ gene, the production of these mature cardiolipins is impaired and the mitochondrial membrane loses integrity potentially leading to cell death. Untargeted analysis of lymphoblast cell lines isolated and immortalized from Barth patients compared to healthy patients showed ether lipids are affected: a monoether phosphatidylcholine accumulated and a monoether phosphatidylethanolamine depleted. Targeted analysis showed that phospholipids, for example phosphatidylglycerols and phosphatidylethanolamines accumulated. These observations along with others are outlined in Chapter 2."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79814"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["analytical chemistry","biochemistry","medicinal chemistry"],"dc:title":["LC-MS Based Lipidomics to Study the Lipids Involved in Barth Syndrome and the Lipid Composition of Different Serum Types"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:19Z"}