{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79400"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79400","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"In Vitro Evaluation of Virulence Characteristics of Lipopolysaccharide from Two Different Clinical Isolates of Tannerella forsythia","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Albanyan, Hajar"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Sharma, Ashu","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-04-04T20:32:24Z","date_published":"2019-04-04T20:32:24Z","updated_at":"2026-07-27T19:05:16Z","subjects":["micro"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79400","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sharma, Ashu","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Albanyan, Hajar"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-04-04T20:32:24Z","2019","2019-01-16 13:33:46"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["micro"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79400"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Lipopolysaccharide (LPS) is one of the dominant virulence factors of Gram-negative bacteria that is implicated in the stimulation of inflammatory cytokines and inflammatory bone resorption. The inflammatory activity of an LPS molecule depends on its structure and chemical composition. In the context of periodontal diseases, LPS derived from Gram-negative periodontal bacteria can initiate inflammatory alveolar bone loss. In this regard, the knowledge about the virulence potential of different forms of LPS from the periodontal pathogen Tannerella forsythia is lacking. The aim of this study was to investigate if LPS molecules from different clinical isolates of T. forsythia differ in their inflammatory activity and ability to promote osteoclastogenesis. To reach this aim, LPS was isolated from two different clinical isolates (namely, UB4 and UB20) and a laboratory strain (ATCC 43037) of T. forsythia and analyzed by SDS-polyacrylamide gel electrophoresis. THP-1 derived macrophages were then stimulated with different LPS molecules and the secreted cytokines were analyzed by ELISA. In addition, mouse RAW 264.7 macrophages were challenged with LPS molecules and osteoclast differentiation was observed by light microscopy following TRAP staining. The results showed that macrophages stimulated with LPS from the clinical isolate UB20 induced increasing levels of IL-1b, TNFa, and IL-6 and osteoclast differentiation in a dose dependent manner as compared to the LPS from the clinical isolate UB4 as well as the laboratory strain ATCC 43037. It was deduced from this study that UB20 is a potential virulent strain of T. forsythia, and infection by this strain might possibly lead to intense periodontal and periapical inflammation as well as periodontal alveolar bone loss due to elevated induction of proinflammatory cytokines and enhanced osteoclastogenesis process."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["In Vitro Evaluation of Virulence Characteristics of Lipopolysaccharide from Two Different Clinical Isolates of Tannerella forsythia"]}]}],"canonical_facts":{"dc:contributor":["Sharma, Ashu","Oral Sciences"],"dc:creator":["Albanyan, Hajar"],"dc:date":["2019-04-04T20:32:24Z","2019","2019-01-16 13:33:46"],"dc:description":["M.S.","Lipopolysaccharide (LPS) is one of the dominant virulence factors of Gram-negative bacteria that is implicated in the stimulation of inflammatory cytokines and inflammatory bone resorption. The inflammatory activity of an LPS molecule depends on its structure and chemical composition. In the context of periodontal diseases, LPS derived from Gram-negative periodontal bacteria can initiate inflammatory alveolar bone loss. In this regard, the knowledge about the virulence potential of different forms of LPS from the periodontal pathogen Tannerella forsythia is lacking. The aim of this study was to investigate if LPS molecules from different clinical isolates of T. forsythia differ in their inflammatory activity and ability to promote osteoclastogenesis. To reach this aim, LPS was isolated from two different clinical isolates (namely, UB4 and UB20) and a laboratory strain (ATCC 43037) of T. forsythia and analyzed by SDS-polyacrylamide gel electrophoresis. THP-1 derived macrophages were then stimulated with different LPS molecules and the secreted cytokines were analyzed by ELISA. In addition, mouse RAW 264.7 macrophages were challenged with LPS molecules and osteoclast differentiation was observed by light microscopy following TRAP staining. The results showed that macrophages stimulated with LPS from the clinical isolate UB20 induced increasing levels of IL-1b, TNFa, and IL-6 and osteoclast differentiation in a dose dependent manner as compared to the LPS from the clinical isolate UB4 as well as the laboratory strain ATCC 43037. It was deduced from this study that UB20 is a potential virulent strain of T. forsythia, and infection by this strain might possibly lead to intense periodontal and periapical inflammation as well as periodontal alveolar bone loss due to elevated induction of proinflammatory cytokines and enhanced osteoclastogenesis process."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79400"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["micro"],"dc:title":["In Vitro Evaluation of Virulence Characteristics of Lipopolysaccharide from Two Different Clinical Isolates of Tannerella forsythia"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:16Z"}