{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79352"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79352","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"HPLC Study of Amino-Silica Columns","abstract":"M.A.","abstract_html":"M.A.","abstract_has_math":false,"creators":["Garnero, Martin"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Colon, Luis","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-04-04T20:30:44Z","date_published":"2019-04-04T20:30:44Z","updated_at":"2026-07-27T19:05:16Z","subjects":["analytical chemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79352","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Colon, Luis","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Garnero, Martin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-04-04T20:30:44Z","2019","2018-12-16 17:11:05"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["analytical chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79352"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.A.","Silica particles were modified by three different methods to incorporate an aminophenyl moiety on the silica surface. These particles were packed into columns and tested under two high performance liquid chromatographic modes: reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC). One column was prepared using particles modified with p- aminophenyltrimethoxysilane, or p-APTMS (i.e., p-APTMS column). The particles for the second column were modified by means of crafting phenylenediamine (PD) onto silica via diazotization (PD column). The third column was prepared by a hydrosilation reaction of a surface hydride- terminated silica (known as type-C silica) with 4-vinylaniline (aminophenyl column). These columns contain functionalities capable of hydrophilic, -, and ion exchange interactions. The retentive behaviors of commercial amino columns were investigated under similar conditions for comparison. We investigated parameters related to mobile phase composition; these included acetonitrile content, type of salt and concentration used as modifier, pH, and temperature. The aminophenyl and PD columns retained and separated hydrophobic and hydrophilic compounds under reverse phase and HILIC conditions, with selectivity differences arising from stationary phase differences. Overall, the aminophenyl column seemed to favor HILIC behavior, while the PD column showed potential for both reverse phase and HILIC applications. The p-APTMS column showed strong reverse-phase behavior."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["HPLC Study of Amino-Silica Columns"]}]}],"canonical_facts":{"dc:contributor":["Colon, Luis","Chemistry"],"dc:creator":["Garnero, Martin"],"dc:date":["2019-04-04T20:30:44Z","2019","2018-12-16 17:11:05"],"dc:description":["M.A.","Silica particles were modified by three different methods to incorporate an aminophenyl moiety on the silica surface. These particles were packed into columns and tested under two high performance liquid chromatographic modes: reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC). One column was prepared using particles modified with p- aminophenyltrimethoxysilane, or p-APTMS (i.e., p-APTMS column). The particles for the second column were modified by means of crafting phenylenediamine (PD) onto silica via diazotization (PD column). The third column was prepared by a hydrosilation reaction of a surface hydride- terminated silica (known as type-C silica) with 4-vinylaniline (aminophenyl column). These columns contain functionalities capable of hydrophilic, -, and ion exchange interactions. The retentive behaviors of commercial amino columns were investigated under similar conditions for comparison. We investigated parameters related to mobile phase composition; these included acetonitrile content, type of salt and concentration used as modifier, pH, and temperature. The aminophenyl and PD columns retained and separated hydrophobic and hydrophilic compounds under reverse phase and HILIC conditions, with selectivity differences arising from stationary phase differences. Overall, the aminophenyl column seemed to favor HILIC behavior, while the PD column showed potential for both reverse phase and HILIC applications. The p-APTMS column showed strong reverse-phase behavior."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79352"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["analytical chemistry"],"dc:title":["HPLC Study of Amino-Silica Columns"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:16Z"}