{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/79344"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/79344","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Role of the Triphosphatase Domain of the Vacinnia Capping Enzyme During Early Gene Transcription Termination","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Bowman, Rachel"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gollnick, Paul","Biological Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-04-04T20:30:37Z","date_published":"2019-04-04T20:30:37Z","updated_at":"2026-07-27T19:05:16Z","subjects":["biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/79344","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gollnick, Paul","Biological Sciences"]},{"key":"dc:creator","label":"Author","values":["Bowman, Rachel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-04-04T20:30:37Z","2018","2018-11-25 20:57:05"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/79344"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Poxviruses are large, double stranded DNA viruses that replicate exclusively in the cytoplasm of the host cell. All poxviruses must encode their own transcription and replication machinery. Vaccinia virus, a prototypic poxvirus, encodes for its own DNA-dependent RNA polymerase (RNAP). Because of homologies between the vaccinia RNAP and eukaryotic RNA polymerases, vaccinia provides a model system with which to study eukaryotic transcription. Transcription of vaccinia genes progresses temporally through Early, Intermediate, and Late genes. During early gene transcription, termination occurs in a signal dependent manner. RNA 3’ end formation occurs ~20-50 residues downstream from a U5NU sequence transcribed in the nascent RNA. In addition to this termination signal, several other viral factors are also required for proper termination. First, the early gene specific form of the viral RNAP is required. The early gene form of the polymerase contains 8 core subunits that are also involved in intermediate and late transcription plus an early gene specific subunit, Rap94. Another viral protein, NPHI, is also necessary for termination. NPHI is a single stranded DNA-dependent ATPase that releases transcripts from paused transcription elongation complexes through a forward translocation mechanism. The final component required for termination is the viral mRNA capping enzyme (CE), a heterodimer of the D1R and D12L gene products.. CE interacts with the U5NU sequence within the RNA and, through an unknown mechanism, induces pausing of RNAP in response to this signal."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of the Triphosphatase Domain of the Vacinnia Capping Enzyme During Early Gene Transcription Termination"]}]}],"canonical_facts":{"dc:contributor":["Gollnick, Paul","Biological Sciences"],"dc:creator":["Bowman, Rachel"],"dc:date":["2019-04-04T20:30:37Z","2018","2018-11-25 20:57:05"],"dc:description":["Ph.D.","Poxviruses are large, double stranded DNA viruses that replicate exclusively in the cytoplasm of the host cell. All poxviruses must encode their own transcription and replication machinery. Vaccinia virus, a prototypic poxvirus, encodes for its own DNA-dependent RNA polymerase (RNAP). Because of homologies between the vaccinia RNAP and eukaryotic RNA polymerases, vaccinia provides a model system with which to study eukaryotic transcription. Transcription of vaccinia genes progresses temporally through Early, Intermediate, and Late genes. During early gene transcription, termination occurs in a signal dependent manner. RNA 3’ end formation occurs ~20-50 residues downstream from a U5NU sequence transcribed in the nascent RNA. In addition to this termination signal, several other viral factors are also required for proper termination. First, the early gene specific form of the viral RNAP is required. The early gene form of the polymerase contains 8 core subunits that are also involved in intermediate and late transcription plus an early gene specific subunit, Rap94. Another viral protein, NPHI, is also necessary for termination. NPHI is a single stranded DNA-dependent ATPase that releases transcripts from paused transcription elongation complexes through a forward translocation mechanism. The final component required for termination is the viral mRNA capping enzyme (CE), a heterodimer of the D1R and D12L gene products.. CE interacts with the U5NU sequence within the RNA and, through an unknown mechanism, induces pausing of RNAP in response to this signal."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/79344"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["biology"],"dc:title":["The Role of the Triphosphatase Domain of the Vacinnia Capping Enzyme During Early Gene Transcription Termination"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:16Z"}