{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78521"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78521","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Comparing Interactions of Commensal and Pathogenic Oral Microbes with Phagocytic Cells","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Aloraini, Saleh; 0000-0001-6744-3808"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Kay, Jason","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-10-26T02:54:45Z","date_published":"2018-10-26T02:54:45Z","updated_at":"2026-07-27T19:05:12Z","subjects":["dentistry","biology","immunology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78521","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kay, Jason","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Aloraini, Saleh; 0000-0001-6744-3808"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-10-26T02:54:45Z","2018","2018-07-18 13:57:41"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry","biology","immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78521"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","AbstractBackground: Periodontal disease is one of the most prevalent diseases in humans. It has a complex etiology, though the main pathogenic factors are oral bacteria which are present in dental biofilm deposited on tooth surfaces. Some oral microbes, such as P. gingivalis, have the ability to evade the immune response and, as a keystone pathogen, inititates dysbiosis in the oral biofilm, while others, including some of which are generally considered commensal, such as S. gordonii. Against this background are a number of innate immune cells that interact with oral microbes, including oral macrophages and dendritic cells (DCs). Understanding the differences between interactions between various host cell interactions with oral microbes may lead to an increased understanding of the importance of each cell type in their roles in the development of periodontal disease. Aims: Compare rates of phagosomal killing ability and the inflammatory response of macrophages and DCs in response to traditionally pathogenic P. gingivalis and commensal S. gordonii. Materials and Methods: The bacterial uptake and intracellular clearance capability of macrophages was determined by an antibiotic protection-based assay. ELISA assays were used to test for cytokine release differences between the macrophage types upon interaction with P. gingivalis and S. gordonii. Results: P. gingivalis and S. gordonii are different in cytokine stimulation and survival within cells. P. gingivalis tended to survive more within the phagocytic cells, and it also induced more modest levels of cytokine released from the cells. Conclusion: This study directly compares differences in the ability of oral microbes (i.e. P. gingivalis and S. gordonii) to evade, inhibit, alter and survive the innate immune response by a number of phagocytes, which adds to the information on differences in oral microbe ability to alter inflammation resolution."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparing Interactions of Commensal and Pathogenic Oral Microbes with Phagocytic Cells"]}]}],"canonical_facts":{"dc:contributor":["Kay, Jason","Oral Sciences"],"dc:creator":["Aloraini, Saleh; 0000-0001-6744-3808"],"dc:date":["2018-10-26T02:54:45Z","2018","2018-07-18 13:57:41"],"dc:description":["M.S.","AbstractBackground: Periodontal disease is one of the most prevalent diseases in humans. It has a complex etiology, though the main pathogenic factors are oral bacteria which are present in dental biofilm deposited on tooth surfaces. Some oral microbes, such as P. gingivalis, have the ability to evade the immune response and, as a keystone pathogen, inititates dysbiosis in the oral biofilm, while others, including some of which are generally considered commensal, such as S. gordonii. Against this background are a number of innate immune cells that interact with oral microbes, including oral macrophages and dendritic cells (DCs). Understanding the differences between interactions between various host cell interactions with oral microbes may lead to an increased understanding of the importance of each cell type in their roles in the development of periodontal disease. Aims: Compare rates of phagosomal killing ability and the inflammatory response of macrophages and DCs in response to traditionally pathogenic P. gingivalis and commensal S. gordonii. Materials and Methods: The bacterial uptake and intracellular clearance capability of macrophages was determined by an antibiotic protection-based assay. ELISA assays were used to test for cytokine release differences between the macrophage types upon interaction with P. gingivalis and S. gordonii. Results: P. gingivalis and S. gordonii are different in cytokine stimulation and survival within cells. P. gingivalis tended to survive more within the phagocytic cells, and it also induced more modest levels of cytokine released from the cells. Conclusion: This study directly compares differences in the ability of oral microbes (i.e. P. gingivalis and S. gordonii) to evade, inhibit, alter and survive the innate immune response by a number of phagocytes, which adds to the information on differences in oral microbe ability to alter inflammation resolution."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78521"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry","biology","immunology"],"dc:title":["Comparing Interactions of Commensal and Pathogenic Oral Microbes with Phagocytic Cells"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:12Z"}