{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78508"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78508","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"The Effects of Vitamin D3 Metabolites on Titanium Surfaces","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Basha, Nadia"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Dziak, Rosemary","Oral Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-10-26T02:54:33Z","date_published":"2018-10-26T02:54:33Z","updated_at":"2026-07-27T19:05:09Z","subjects":["dentistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78508","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dziak, Rosemary","Oral Sciences"]},{"key":"dc:creator","label":"Author","values":["Basha, Nadia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-10-26T02:54:33Z","2018","2018-07-08 13:58:27"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78508"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","The goal for a successful osseointegration of bone with implants is for them to have the ability to induce, enhance, and accelerate bone healing. Their surfaces should create a reservoir of interfacial matrix filled with bone with the characteristics of compositional and structural quality of matured bone. There are several approaches that have been adapted to modify the surfaces of implants and titanium mesh graft materials including biochemical techniques to enhance bone integration.The main objective of using biochemical surface modification is to immobilize certain molecules such as proteins, peptides, or enzymes on biomaterials for the purpose of stimulating specific tissue and cell responses. Additionally, this modification helps to control the tissue implant interface through delivery of molecules into the interface directly and thus enhance bone healing and formation.Vitamin D is a biocompatible molecule which is readily available by the exposure of the skin to the sun light and is stored in the body. It plays an important role in bone metabolism and calcium mineral homeostasis. It has been suggested that Vitamin D and its metabolites have direct effects on osteogenesis since vitamin D receptors (VDR) are present on osteoblasts and osteoclast precursors. Even though there are both in vivo and in vitro studies that suggest that Vitamin D and its metabolites have effects on osteogenesis, their biologic responses have not yet been understood fully especially when adsorbed to implantable materials. The aim of this study was to coat pure, uniformly cut titanium mesh surfaces with a range of known concentrations of Vitamin D metabolites (25-OHD3 and 1,25(OH)2D3) and assess the differentiation of human osteoblastic cells in the presence of the coated surfaces in comparison to non-coated (control) titanium mesh surfaces. For these assessments, a standard growth/activity (MTT) assay, and alkaline phosphatase and mineralization assays, well-characterized markers of osteoblastic cell differentiation were used. Our results suggest that the application of 25-OHD3 as well as 1α,25(OH)2D3 at appropriate concentrations, can have small, significant effects to increase osteoblastic cell differentiation on titanium mesh. However, these results are not consistently observed in all the experiments conducted with human primary calvarial osteoblasts. The adaptation of these results to clinical use requires further testing with emphasis on in vivo models, but the in vitro studies presented here add to an emerging body of literature suggesting a beneficial effect of Vitamin D metabolites in osseointegration, but that appropriate conditions have to be determined for consistent results."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Effects of Vitamin D3 Metabolites on Titanium Surfaces"]}]}],"canonical_facts":{"dc:contributor":["Dziak, Rosemary","Oral Sciences"],"dc:creator":["Basha, Nadia"],"dc:date":["2018-10-26T02:54:33Z","2018","2018-07-08 13:58:27"],"dc:description":["M.S.","The goal for a successful osseointegration of bone with implants is for them to have the ability to induce, enhance, and accelerate bone healing. Their surfaces should create a reservoir of interfacial matrix filled with bone with the characteristics of compositional and structural quality of matured bone. There are several approaches that have been adapted to modify the surfaces of implants and titanium mesh graft materials including biochemical techniques to enhance bone integration.The main objective of using biochemical surface modification is to immobilize certain molecules such as proteins, peptides, or enzymes on biomaterials for the purpose of stimulating specific tissue and cell responses. Additionally, this modification helps to control the tissue implant interface through delivery of molecules into the interface directly and thus enhance bone healing and formation.Vitamin D is a biocompatible molecule which is readily available by the exposure of the skin to the sun light and is stored in the body. It plays an important role in bone metabolism and calcium mineral homeostasis. It has been suggested that Vitamin D and its metabolites have direct effects on osteogenesis since vitamin D receptors (VDR) are present on osteoblasts and osteoclast precursors. Even though there are both in vivo and in vitro studies that suggest that Vitamin D and its metabolites have effects on osteogenesis, their biologic responses have not yet been understood fully especially when adsorbed to implantable materials. The aim of this study was to coat pure, uniformly cut titanium mesh surfaces with a range of known concentrations of Vitamin D metabolites (25-OHD3 and 1,25(OH)2D3) and assess the differentiation of human osteoblastic cells in the presence of the coated surfaces in comparison to non-coated (control) titanium mesh surfaces. For these assessments, a standard growth/activity (MTT) assay, and alkaline phosphatase and mineralization assays, well-characterized markers of osteoblastic cell differentiation were used. Our results suggest that the application of 25-OHD3 as well as 1α,25(OH)2D3 at appropriate concentrations, can have small, significant effects to increase osteoblastic cell differentiation on titanium mesh. However, these results are not consistently observed in all the experiments conducted with human primary calvarial osteoblasts. The adaptation of these results to clinical use requires further testing with emphasis on in vivo models, but the in vitro studies presented here add to an emerging body of literature suggesting a beneficial effect of Vitamin D metabolites in osseointegration, but that appropriate conditions have to be determined for consistent results."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78508"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["dentistry"],"dc:title":["The Effects of Vitamin D3 Metabolites on Titanium Surfaces"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:09Z"}