{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78409"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78409","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Bioorthogonal Chemistry for Protein Labeling in Live Cells","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Lewandowski-Baird, Tracey"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Lin, Qing","Chemistry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-10-24T20:24:25Z","date_published":"2018-10-24T20:24:25Z","updated_at":"2026-07-27T19:05:09Z","subjects":["organic chemistry","biochemistry"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78409","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lin, Qing","Chemistry"]},{"key":"dc:creator","label":"Author","values":["Lewandowski-Baird, Tracey"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-10-24T20:24:25Z","2018","2018-07-28 13:00:37"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["organic chemistry","biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78409"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Chapter one provides an overview of bioorthogonal reactions commonly used in the chemical biology field. Chapter two describes the synthesis of a BODIPY-tetrazole and its use as an “off-on” reporter of hydrogen peroxide in live cells. The BODIPY–pyrazoline sensor is generated in situ in cellular systems using photoclick chemistry, and subsequent oxidation of the pyrazoline to a pyrazole serves as the basis for detection of hydrogen peroxide. Chapter three focuses on the optimization of photoclick chemistry in the context of the tetrazole structure. A sterically shielded nitrile imine was designed that favors the 1,3-dipolar cycloaddition over the competing nucleophilic addition. The utility of this sterically shielded nitrile imine in rapid (∼1 min) bioorthogonal labeling of glucagon receptor in live mammalian cells was demonstrated. Chapter four focuses on bioorthogonal modification of class B G-Protein Coupled Receptors (GPCRs), specifically the glucagon-like peptide 1 receptor (GLP-1R). A FRET-based approach was undertaken with a goal to study the biophysical properties on the native receptor in living cells. A strained alkene amino acid was site-specifically incorporated into the extracellular loops of GLP-1R using amber codon suppression. Dual labeling of GLP-1R was achieved using SNAP-tag technology along with bioorthogonal tetrazine ligation.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Bioorthogonal Chemistry for Protein Labeling in Live Cells"]}]}],"canonical_facts":{"dc:contributor":["Lin, Qing","Chemistry"],"dc:creator":["Lewandowski-Baird, Tracey"],"dc:date":["2018-10-24T20:24:25Z","2018","2018-07-28 13:00:37"],"dc:description":["Ph.D.","Chapter one provides an overview of bioorthogonal reactions commonly used in the chemical biology field. Chapter two describes the synthesis of a BODIPY-tetrazole and its use as an “off-on” reporter of hydrogen peroxide in live cells. The BODIPY–pyrazoline sensor is generated in situ in cellular systems using photoclick chemistry, and subsequent oxidation of the pyrazoline to a pyrazole serves as the basis for detection of hydrogen peroxide. Chapter three focuses on the optimization of photoclick chemistry in the context of the tetrazole structure. A sterically shielded nitrile imine was designed that favors the 1,3-dipolar cycloaddition over the competing nucleophilic addition. The utility of this sterically shielded nitrile imine in rapid (∼1 min) bioorthogonal labeling of glucagon receptor in live mammalian cells was demonstrated. Chapter four focuses on bioorthogonal modification of class B G-Protein Coupled Receptors (GPCRs), specifically the glucagon-like peptide 1 receptor (GLP-1R). A FRET-based approach was undertaken with a goal to study the biophysical properties on the native receptor in living cells. A strained alkene amino acid was site-specifically incorporated into the extracellular loops of GLP-1R using amber codon suppression. Dual labeling of GLP-1R was achieved using SNAP-tag technology along with bioorthogonal tetrazine ligation.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78409"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["organic chemistry","biochemistry"],"dc:title":["Bioorthogonal Chemistry for Protein Labeling in Live Cells"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:09Z"}