{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78404"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78404","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Effects of Excessive Cholesterol Exposure and Phytosterol Supplementation During Lactation On Offspring Metabolic Health In ApoE Deficient Mice","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Ma, Min"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Rideout, Todd","Exercise and Nutrition Sciences"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-10-24T19:54:46Z","date_published":"2018-10-24T19:54:46Z","updated_at":"2026-07-27T19:05:09Z","subjects":["nutrition"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78404","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rideout, Todd","Exercise and Nutrition Sciences"]},{"key":"dc:creator","label":"Author","values":["Ma, Min"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-10-24T19:54:46Z","2018","2018-07-09 16:58:51"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78404"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Maternal overt hypercholesterolemia during pregnancy can place the offspring at a higher risk of a variety of metabolic dysfunction in early and later life, including cardiovascular disease (CVD), dyslipidemia, non-alcoholic fatty liver disease, and obesity. To address this critical health care priority, a better understanding of the pathology of maternal hypercholesterolemia and the period-specific programming effects are warranted in the hope of developing safe and effective cholesterol management strategies to improve the health of pregnant women and their offspring. Previous work has demonstrated the adverse effects of excessive cholesterol (CH) and potential protective effects of phytosterols (PS) supplementation during gestation and lactation. Therefore, this thesis work aimed to examine the effect of excessive cholesterol exposure with and without PS supplementation during the lactation only on lipid metabolism in newly weaned offspring using an apolipoprotein E (apoE) deficient mouse model. Twenty-four mature (2-month old) female apoE-/- mice fed a standard chow (CON) throughout gestation. At birth, litters were cross-fostered to pair-bred mothers fed either the chow, a cholesterol-supplemented diet (0.15%, w/w, CH), or the CH diet with PS (2%, w/w, CH/PS). At weaning (postnatal day 21), blood and liver tissue of the offspring were collected for lipid analyses. Compared to CON pups, offspring suckled by cholesterol-fed dams demonstrated a reduction (p< 0.05) in serum high-density lipoprotein cholesterol (HDL-C), increased total cholesterol to HDL (TC: HDL) ratio, and enhanced hepatic cholesterol (p = 0.05). On the other hand, offspring suckled by PS-supplemented mothers had a normalized HDL-C and TC: HDL-C ratio and a lower hepatic CH compared with CON offspring. In conclusion, excessive cholesterol exposure during lactation is sufficient to cause adverse effects on the lipid profile of offspring, however, these responses can be alleviated by maternal PS supplementation.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Effects of Excessive Cholesterol Exposure and Phytosterol Supplementation During Lactation On Offspring Metabolic Health In ApoE Deficient Mice"]}]}],"canonical_facts":{"dc:contributor":["Rideout, Todd","Exercise and Nutrition Sciences"],"dc:creator":["Ma, Min"],"dc:date":["2018-10-24T19:54:46Z","2018","2018-07-09 16:58:51"],"dc:description":["M.S.","Maternal overt hypercholesterolemia during pregnancy can place the offspring at a higher risk of a variety of metabolic dysfunction in early and later life, including cardiovascular disease (CVD), dyslipidemia, non-alcoholic fatty liver disease, and obesity. To address this critical health care priority, a better understanding of the pathology of maternal hypercholesterolemia and the period-specific programming effects are warranted in the hope of developing safe and effective cholesterol management strategies to improve the health of pregnant women and their offspring. Previous work has demonstrated the adverse effects of excessive cholesterol (CH) and potential protective effects of phytosterols (PS) supplementation during gestation and lactation. Therefore, this thesis work aimed to examine the effect of excessive cholesterol exposure with and without PS supplementation during the lactation only on lipid metabolism in newly weaned offspring using an apolipoprotein E (apoE) deficient mouse model. Twenty-four mature (2-month old) female apoE-/- mice fed a standard chow (CON) throughout gestation. At birth, litters were cross-fostered to pair-bred mothers fed either the chow, a cholesterol-supplemented diet (0.15%, w/w, CH), or the CH diet with PS (2%, w/w, CH/PS). At weaning (postnatal day 21), blood and liver tissue of the offspring were collected for lipid analyses. Compared to CON pups, offspring suckled by cholesterol-fed dams demonstrated a reduction (p< 0.05) in serum high-density lipoprotein cholesterol (HDL-C), increased total cholesterol to HDL (TC: HDL) ratio, and enhanced hepatic cholesterol (p = 0.05). On the other hand, offspring suckled by PS-supplemented mothers had a normalized HDL-C and TC: HDL-C ratio and a lower hepatic CH compared with CON offspring. In conclusion, excessive cholesterol exposure during lactation is sufficient to cause adverse effects on the lipid profile of offspring, however, these responses can be alleviated by maternal PS supplementation.","**To request an accessible version of the file(s) associated with this item, contact library@buffalo.edu. Please include the item's persistent URL [http://hdl.handle.net/. . .] in your request.**"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78404"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["nutrition"],"dc:title":["Effects of Excessive Cholesterol Exposure and Phytosterol Supplementation During Lactation On Offspring Metabolic Health In ApoE Deficient Mice"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:09Z"}