{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78123"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78123","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Functional Genomic Screens for Novel Metastasis Suppressor Genes in Breast and Prostate Cancer Models","abstract":"Ph.D.","abstract_html":"Ph.D.","abstract_has_math":false,"creators":["Withers, Henry; 0000-0002-9746-9352"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gelman, Irwin","Roswell Park"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-06-28T20:34:24Z","date_published":"2018-06-28T20:34:24Z","updated_at":"2026-07-27T19:05:09Z","subjects":["cellular biology","genetics","molecular physics","molecular biology"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78123","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gelman, Irwin","Roswell Park"]},{"key":"dc:creator","label":"Author","values":["Withers, Henry; 0000-0002-9746-9352"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-06-28T20:34:24Z","2018","2018-05-18 12:07:46"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:type","label":"Dc Type","values":["Text","Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["cellular biology","genetics","molecular physics","molecular biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78123"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Ph.D.","Metastasis is the predominant cause of mortality in many cancers, including prostate and breast cancers. With a substantial number of men and women being diagnosed with these cancers each year in the United States, there is a need for effective methods to rapidly identify key regulators of the metastatic cascade that can subsequently be employed in diagnostic, prognostic, and therapeutic applications. This has become increasingly important as we come to understand that many metastatic events occur earlier in disease progression than originally thought. Functional genomic screens have arisen as an effective means to rapidly screen for critical genes controlling a specified biology. Loss of function screens, such as RNAi libraries, are particularly useful for the identification of suppressor genes. We utilized genome-wide RNAi screens to identify negative regulators of metastasis, defined as metastasis suppressor genes, with in vivo and in vitro breast and prostate cancer models. We performed multiple screens assessing the individual contributions of genes in Matrigel invasion (T-47D), initial colonization of lungs by prostate cancer cells (LNCaP) in a mouse model, and complete metastasis from orthotopic tumor to distant metastases in vivo (LNCaP)."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Functional Genomic Screens for Novel Metastasis Suppressor Genes in Breast and Prostate Cancer Models"]}]}],"canonical_facts":{"dc:contributor":["Gelman, Irwin","Roswell Park"],"dc:creator":["Withers, Henry; 0000-0002-9746-9352"],"dc:date":["2018-06-28T20:34:24Z","2018","2018-05-18 12:07:46"],"dc:description":["Ph.D.","Metastasis is the predominant cause of mortality in many cancers, including prostate and breast cancers. With a substantial number of men and women being diagnosed with these cancers each year in the United States, there is a need for effective methods to rapidly identify key regulators of the metastatic cascade that can subsequently be employed in diagnostic, prognostic, and therapeutic applications. This has become increasingly important as we come to understand that many metastatic events occur earlier in disease progression than originally thought. Functional genomic screens have arisen as an effective means to rapidly screen for critical genes controlling a specified biology. Loss of function screens, such as RNAi libraries, are particularly useful for the identification of suppressor genes. We utilized genome-wide RNAi screens to identify negative regulators of metastasis, defined as metastasis suppressor genes, with in vivo and in vitro breast and prostate cancer models. We performed multiple screens assessing the individual contributions of genes in Matrigel invasion (T-47D), initial colonization of lungs by prostate cancer cells (LNCaP) in a mouse model, and complete metastasis from orthotopic tumor to distant metastases in vivo (LNCaP)."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78123"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["cellular biology","genetics","molecular physics","molecular biology"],"dc:title":["Functional Genomic Screens for Novel Metastasis Suppressor Genes in Breast and Prostate Cancer Models"],"dc:type":["Text","Dissertation"]},"updated_at":"2026-07-27T19:05:09Z"}