{"id":{"repo_id":"buffalo","oai_identifier":"oai:ubir.buffalo.edu:10477/78094"},"canonical_url":"https://search.dev.ndltd.org/etd/buffalo/oai:ubir.buffalo.edu:10477/78094","repository":{"repo_id":"buffalo","name":"Buffalo","base_url":"https://ubir.buffalo.edu/oai/request"},"display":{"title":"Role of nuclear-FGFR1 in Schizophrenia and Ontogenic Genome Programming – A Computational Study","abstract":"M.S.","abstract_html":"M.S.","abstract_has_math":false,"creators":["Dhiman, Siddhartha; 0000-0001-6957-8765"],"institution":"State University of New York at Buffalo","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Stachowiak, Michal","Biomedical Engineering"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-06-28T20:33:59Z","date_published":"2018-06-28T20:33:59Z","updated_at":"2026-07-27T19:05:07Z","subjects":["biomedical engineering","biostatistics","bioinformatics"],"languages":["eng"],"rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10477/78094","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Stachowiak, Michal","Biomedical Engineering"]},{"key":"dc:creator","label":"Author","values":["Dhiman, Siddhartha; 0000-0001-6957-8765"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-06-28T20:33:59Z","2018","2018-05-18 00:09:01"]},{"key":"dc:publisher","label":"Institution","values":["State University of New York at Buffalo"]},{"key":"dc:relation","label":"Dc Relation","values":["Supplementary files: Dhiman-supplementary-materials-miRNA-targets.zip (zipped spreadsheets), Excel files containing list of all miRNAs and their miRNA targets"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biomedical engineering","biostatistics","bioinformatics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/10477/78094"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["M.S.","Earlier studies from schizophrenia patients have shown that the disorder causes changes in the genome at proneuronal stage – best progenitor cells commit to neural cells. In particular, the mRNA transcriptome nuclear FGFR1 (nFGFR1) was found to be dysregulated. Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) derived cerebral organoid from four control and three schizophrenia patients were grown to model the first trimester of in utero brain development. These organoids were tagged to show Ki67 + neural progenitor cells (NPCs) and calretinin inter neurons in the cortical plate (CP). Using computational algorithms to detect cells and characterize them, it was discovered that NPCs in schizophrenia modeled organoids abnormally in comparison to those in control organoids. The matured inter-neurons in the CP showed decreased intracortical connectivity denoted by changes in their orientation. Further effects of nFGFR1 on the global genome programming were studied using fold change and correlational analyses. nFGFR1 was either under expressed or over expressed using tyrosine kinase domain removal (TK-) or the addition of nuclear localization signal (+NLS) respectively, in both NPCs and NCCs. It was observed that nFGFR1 acts like a complex circuit comprising of a bandpass filter and a proportional (P) controller to modulate gene-gene coordination. In addition, miRNA coordination was also analyzed in relation to mRNAs using similar correlational analyses to reveal that the entire genome maintains a homeostatic state that preserves the miRNA to mRNA control such that the constant of proportionality between them is unaffected by changes in genome."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Role of nuclear-FGFR1 in Schizophrenia and Ontogenic Genome Programming – A Computational Study"]}]}],"canonical_facts":{"dc:contributor":["Stachowiak, Michal","Biomedical Engineering"],"dc:creator":["Dhiman, Siddhartha; 0000-0001-6957-8765"],"dc:date":["2018-06-28T20:33:59Z","2018","2018-05-18 00:09:01"],"dc:description":["M.S.","Earlier studies from schizophrenia patients have shown that the disorder causes changes in the genome at proneuronal stage – best progenitor cells commit to neural cells. In particular, the mRNA transcriptome nuclear FGFR1 (nFGFR1) was found to be dysregulated. Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) derived cerebral organoid from four control and three schizophrenia patients were grown to model the first trimester of in utero brain development. These organoids were tagged to show Ki67 + neural progenitor cells (NPCs) and calretinin inter neurons in the cortical plate (CP). Using computational algorithms to detect cells and characterize them, it was discovered that NPCs in schizophrenia modeled organoids abnormally in comparison to those in control organoids. The matured inter-neurons in the CP showed decreased intracortical connectivity denoted by changes in their orientation. Further effects of nFGFR1 on the global genome programming were studied using fold change and correlational analyses. nFGFR1 was either under expressed or over expressed using tyrosine kinase domain removal (TK-) or the addition of nuclear localization signal (+NLS) respectively, in both NPCs and NCCs. It was observed that nFGFR1 acts like a complex circuit comprising of a bandpass filter and a proportional (P) controller to modulate gene-gene coordination. In addition, miRNA coordination was also analyzed in relation to mRNAs using similar correlational analyses to reveal that the entire genome maintains a homeostatic state that preserves the miRNA to mRNA control such that the constant of proportionality between them is unaffected by changes in genome."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/10477/78094"],"dc:language":["eng"],"dc:publisher":["State University of New York at Buffalo"],"dc:relation":["Supplementary files: Dhiman-supplementary-materials-miRNA-targets.zip (zipped spreadsheets), Excel files containing list of all miRNAs and their miRNA targets"],"dc:rights":["Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.","Copyright retained by author."],"dc:subject":["biomedical engineering","biostatistics","bioinformatics"],"dc:title":["Role of nuclear-FGFR1 in Schizophrenia and Ontogenic Genome Programming – A Computational Study"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T19:05:07Z"}