A novel complex [Ru(bpy)2bpy-py]2+ (where bpy= 2,2’-bipyridine and bpy-py=N-(6-(4-(pyren-1-yl) butanamido) hexyl)-[2,2'-bipyridine]-5-carboxamide))) has been synthesized. The effect of electronically isolating the pyrene from the metal core on the complex’s ability to bind to and photocleave DNA was studied. Other molecules such as 1-pyreneacetic acid and [Ru(bpy)2dep]2+ (where dep= N-(6-aminohexyl)-[2,2'-bipyridine]-5-carboxamide), which share structural similarities to [Ru(bpy)2bpy-py]2+, were also studied to help determine which areas of the [Ru(bpy)2bpy-py]2+ molecule may be responsible for interacting with DNA. Spectroscopic experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding experiments, respectively). Agarose gel electrophoresis, used to assess photocleavage, gave ambiguous results. Additional fluorometric experiments revealed that a transfer of energy from the pyrene portion of the complex to the ruthenium core may be occurring.
","abstract_html":"<p>A novel complex [Ru(bpy)2bpy-py]2+ (where bpy= 2,2’-bipyridine and bpy-py=N-(6-(4-(pyren-1-yl) butanamido) hexyl)-[2,2'-bipyridine]-5-carboxamide))) has been synthesized. The effect of electronically isolating the pyrene from the metal core on the complex’s ability to bind to and photocleave DNA was studied. Other molecules such as 1-pyreneacetic acid and [Ru(bpy)2dep]2+ (where dep= N-(6-aminohexyl)-[2,2'-bipyridine]-5-carboxamide), which share structural similarities to [Ru(bpy)2bpy-py]2+, were also studied to help determine which areas of the [Ru(bpy)2bpy-py]2+ molecule may be responsible for interacting with DNA. Spectroscopic experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding experiments, respectively). Agarose gel electrophoresis, used to assess photocleavage, gave ambiguous results. Additional fluorometric experiments revealed that a transfer of energy from the pyrene portion of the complex to the ruthenium core may be occurring.</p>","abstract_has_math":false,"creators":["Klein, Samantha"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Open Access","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-05-01T07:00:00Z","date_published":"2019-05-01T07:00:00Z","updated_at":"2026-07-24T01:23:46Z","subjects":["Chemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repository.brynmawr.edu/dissertations/199","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Klein, Samantha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.brynmawr.edu/dissertations/199"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A novel complex [Ru(bpy)2bpy-py]2+ (where bpy= 2,2’-bipyridine and bpy-py=N-(6-(4-(pyren-1-yl) butanamido) hexyl)-[2,2'-bipyridine]-5-carboxamide))) has been synthesized. The effect of electronically isolating the pyrene from the metal core on the complex’s ability to bind to and photocleave DNA was studied. Other molecules such as 1-pyreneacetic acid and [Ru(bpy)2dep]2+ (where dep= N-(6-aminohexyl)-[2,2'-bipyridine]-5-carboxamide), which share structural similarities to [Ru(bpy)2bpy-py]2+, were also studied to help determine which areas of the [Ru(bpy)2bpy-py]2+ molecule may be responsible for interacting with DNA. Spectroscopic experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding experiments, respectively). Agarose gel electrophoresis, used to assess photocleavage, gave ambiguous results. Additional fluorometric experiments revealed that a transfer of energy from the pyrene portion of the complex to the ruthenium core may be occurring.
"]},{"key":"dc:title","label":"Title","values":["A Break In Communication: The Synthesis, Characterization, DNA Binding and Photocleavage of a Novel Ruthenium Polypyridyl Complex Containing an Electronically Isolated Pyrene Group"]}]}],"canonical_facts":{"dc:creator":["Klein, Samantha"],"dc:description.abstract":["A novel complex [Ru(bpy)2bpy-py]2+ (where bpy= 2,2’-bipyridine and bpy-py=N-(6-(4-(pyren-1-yl) butanamido) hexyl)-[2,2'-bipyridine]-5-carboxamide))) has been synthesized. The effect of electronically isolating the pyrene from the metal core on the complex’s ability to bind to and photocleave DNA was studied. Other molecules such as 1-pyreneacetic acid and [Ru(bpy)2dep]2+ (where dep= N-(6-aminohexyl)-[2,2'-bipyridine]-5-carboxamide), which share structural similarities to [Ru(bpy)2bpy-py]2+, were also studied to help determine which areas of the [Ru(bpy)2bpy-py]2+ molecule may be responsible for interacting with DNA. Spectroscopic experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding experiments, respectively). Agarose gel electrophoresis, used to assess photocleavage, gave ambiguous results. Additional fluorometric experiments revealed that a transfer of energy from the pyrene portion of the complex to the ruthenium core may be occurring.
"],"dc:identifier":["https://repository.brynmawr.edu/dissertations/199"],"dc:subject":["Chemistry"],"dc:title":["A Break In Communication: The Synthesis, Characterization, DNA Binding and Photocleavage of a Novel Ruthenium Polypyridyl Complex Containing an Electronically Isolated Pyrene Group"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Open Access"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T01:23:46Z"}